The aim of the presented experimental work was to study an acute cell and antibody- mediated immune response in recipients of abdominal aortic grafts treated by a new standardized clinical cryopreservation/slow thawing protocol used in the "Vascular graft transplant program in the Czech Republic" in a rat model. Another aim of our study was to compare the influence of two basic types of conservation protocols used in this program (cryopreservation/slow thawing protocol and cold-stored protocol) on the acute immune response after transplantation of such treated abdominal aortic grafts in rats. Cryopreserved abdominal aortic grafts were transplanted syngeneously between Lewis rats (CRYO-ISO group, cryopreservation period 172.6 days) and allogeneically between Brown-Norway and Lewis rats (CRYO-ALO group, cryopreservation period 179.3 days). The grafts were explanted on day 30 after transplantation and subsequently examined by histological and immunohistochemical methods, focusing on typical signs of acute rejection in the three basic layers of the aortic wall. We monitored the presence of endothelial cells, signs of intimal hyperplasia, tunica media thickness, the presence of necrosis and deposition of imunoglobulin class G in this layer, the number of CD4+, CD8+ and LEW MHC II+ immunocompetent cells...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:447268 |
| Date | January 2021 |
| Creators | Hrubý, Jan |
| Contributors | Matia, Ivan, Oliverius, Martin, Štádler, Petr |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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