Beauveria bassiana is widely used in biological control against the economically significant pests. Detection of morphological and genetic polymorphism among single species and strains in natural environment is important to study of the distribution and the effects in the ecosystem. The aim of this study was to determine the links of "local strains" with ecological aspects of biological control in protected zone of National park and assess the polymorphism of the population monitored in NP Šumava and exotic strains using molecular markers. There were analyzed 39 strains collected in the NP Šumava and the Krkonoše National Park in the Czech Republic and 11 strains from different countries and areas. The polymorphism of these strains was evaluated by different methods based on DNA analysis. In this study, the genetic variability is evaluated by AFLP markers. The results reveal that population from NP Šumava is very closed and the "local strains" can be very well characterized thanks these methods and compared with other strains from different parts of the Czech Republic and other countries. Thanks to these screening analyses, the strain Bba I101was determined for the preparation which will be used for bark beetle (Ips typographus) control in the National Park Šumava in the Czech Republic. This study was supported by grants GACR 521/08/H042, MSM 60076658-06, MZP SP/2d1/41/08.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:53290 |
| Date | January 2010 |
| Creators | KOTLANOVÁ, Kristina |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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