The long-term cattle impact on soil methanotrophs was investigated at cattle overwintering area located at the ecofarm in the South Bohemia. Four sets of soil samples were sampled in spring and fall of 2009 and 2010. Differences in methanotrophic community structure among experimental sites and were investigated by DGGE and MISA. Real-Time PCR was used for estimation of quantity of Type I methanotrophs. The CARD-FISH method was used to estimate the percentage shared and cell counts of Type I and Type II methanotrophs among all prokaryotic cells. Substantial part of the thesis was focused on optimization of methods used.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:55083 |
| Date | January 2011 |
| Creators | NITKULINCOVÁ, Andrea |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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