<p>Plant
biomass is an abundant source of renewable energy, but the efficiency of its
conversion into liquid fuels is low. One reason for this inefficiency is the
recalcitrance of biomass to extraction and saccharification of cell wall
polysaccharides. This recalcitrance is due to the complex and rigid structure
of the plant cell wall. A better understanding of the genes effecting cell wall
composition in bioenergy crops could improve feedstock quality and increase
conversion efficiency. To identify genetic loci associated with biomass quality
traits, we utilized genome-wide association studies (GWAS) in an 840-line <i>Sorghum</i> diversity panel. We identified
several QTL from these GWAS including some for lignin composition and saccharification.
Linkage disequilibrium (LD) analysis suggested that multiple polymorphisms are
driving the association of SNPs within these QTL. Sequencing and further
analysis led to the identification of a SNP within the coding region of a gene
encoding phenylalanine ammonia-lyase (PAL) that creates a premature stop codon
and co-segregates with an increase in the ratio of syringyl (S) to guaiacyl (G)
lignin. A comparison of net PAL activity between lines with and without the
mutation revealed that this mutation results in decreased PAL activity. </p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/7430339 |
| Date | 16 January 2019 |
| Creators | Patrick K. Sweet (5930888) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/ANALYSIS_OF_BIOMASS_COMPOSITION_IN_A_SORGHUM_DIVERSITY_PANEL/7430339 |
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