Rapid determination of drugs effective against bacterial strains is critically important to
stopping further spread of an infection and reducing antibiotic resistance. Antimicrobial
susceptibility testing (AST) is done to determine what type of antibiotic and what concentration
will be effective in treating an infection. Current, growth-dependent, AST methods are reliant on
the growth rate of the bacteria and can take several days to several weeks to get results. A
piezoelectric plate sensor can be used to measure an instant change in the minute physiological
stresses of the bacteria cells when they are exposed to an effective concentration of antibiotic. This
work aims to investigate the feasibility of piezoelectric plate sensors used for instantaneous AST
(iAST) results and develop a technological framework for scaling this technology to a clinical lab
setting. Four Clinical and Laboratory Standards Institute (CLSI) quality control strains of bacteria
were tested with a wide range of antibiotics from various drug classes using the piezoelectric
sensor. Results were obtained within 30 minutes and compared to standard of care AST methods
used in clinical labs, and CLSI prescribed ranges for each strain of bacteria. This thesis will also
discuss a framework for developing more scalable sensors, and challenges associated with the
different sensor designs.
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/8983160 |
| Date | 16 October 2019 |
| Creators | Aline M Elquist (7026050) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/Instantaneous_Antimicrobial_Susceptibility_Testing_Using_Piezoelectric_Sensors/8983160 |
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