In higher eukaryotes, DNA is packaged with histones and other proteins into
chromatin. While this is important in the control of unwanted gene expression,
chromatin also serves as a barrier to many vital functions in the cell. Therefore, cells
have evolved many different types of chromatin remodeling enzymes to contend with
this inhibitory structure and enable gene expression and other functions. The
Saccharomyces cerevisiae PHO5 gene is triggered in response to phosphate starvation.
In this study, I evaluate the chromatin remodeling requirements of this gene with respect
to the multisubunit complexes SWI/SNF and SAGA. I show, for the first time, physical
recruitment of SWI/SNF to the PHO5 promoter. I also demonstrate the role of promoter
occupancy in influencing requirements for chromatin remodeling enzymes. Further, I
describe various interactions between these two complexes at the PHO5 promoter. This
study presents evidence for the first instance of excess recruitment of an ATP-dependent
remodeler potentially compensating for the lack of a histone acetyltransferase.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/3272 |
| Date | 12 April 2006 |
| Creators | Dhasarathy, Archana |
| Contributors | Kladde, Michael P. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Dissertation, text |
| Format | 10159054 bytes, electronic, application/pdf, born digital |
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