We investigate the possibility of extracting accurate functional information such
as local blood oxygenation level using multi-wavelength photoacoustic measurements.
Photoacoustic microscope is utilized to acquire images of microvasculature in smallanimal
skin. Owing to endogenous optical contrast, optical spectral information obtained
from spectral photoacoustic measurements are successfully inverted to yield oxygenation
level in blood. Analysis of error propagation from photoacoustic measurements to
inverted quantities showed minimum inversion error in the optical wavelength region of
570-600 nm. To obtain accurate and vessel size independent blood oxygenation
measurements, transducers with central frequency of more than 25 MHz are needed for
the optical region of 570-600 nm used in this study. The effect of transducer focal
position on accuracy of blood oxygenation level quantification was found to be
negligible. To obtain accurate measurements in vivo, one needs to compensate for
factors such as spectral dependent optical attenuation.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/5851 |
| Date | 17 September 2007 |
| Creators | Sivaramakrishnan, Mathangi |
| Contributors | Wang, Lihong V. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | 2109759 bytes, electronic, application/pdf, born digital |
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