During early pregnancy in ruminants, progesterone (P4) from the corpus
luteum (CL) and interferon tau (IFNT) from the conceptus act on the
endometrium to regulate genes including interferon stimulated genes (ISGs) that
are hypothesized to be important for uterine receptivity and conceptus growth.
Previous custom ovine cDNA array analyses identified candidate genes that
were regulated by pregnancy, P4 and/or IFNT in the ovine uterus.
The first study validated pregnancy, P4 and/or IFNT regulated genes
identified by previous custom ovine cDNA microarray analyses. ACTA2,
COL3A1, POSTN, SPARC, S100A2, STAT5A and TAGLN were examined.
POSTN was upregulated by P4 and S100A2 was downregulated by IFNT.
Moreover, functional studies showed that POSTN stimulated attachment of
ovine trophectoderm cells. However, neither COL3A1, SPARC, ACTA2 nor
TAGLN was regulated by pregnancy, P4 or IFNT in the ovine endometrium.
Collectively, these results confirmed that POSTN and S100A2 are P4 and IFNT
regulated, respectively, and likely involved in uterine receptivity to conceptus
implantation during early pregnancy.
The second study determined expression of ISGs in the CL of pregnant
ewes. MX1, MX2, ISG15, OAS1, and RSAD2 mRNAs were increased on Day
14 of pregnancy and maintained to Day 18, indicating that IFNT induced
expression of ISGs in the CL. These results confirmed that locally produced IFNT has paracrine effects and also endocrine effects on reproductive organs
other than the uterine endometrium and maternal immune system.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/85962 |
| Date | 10 October 2008 |
| Creators | Ahn, Hyo Won |
| Contributors | Bazer, Fuller W., Spencer, Thomas E. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Thesis, text |
| Format | electronic, born digital |
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