In the design of the food irradiation process, the knowledge of the radiation resistance of
the target organism in a specific food commodity is required. The D10-value, the radiation dose
needed to inactivate 90% of the microbial load in the food medium, is used to relate the amount
of absorbed energy to the surviving bacterial population. Numerous experimental studies have
been performed to determine the D10 values of several food-borne microorganisms irradiated
under various conditions. Nevertheless, accurate predictions of D10 values of the pathogens in
food products that have not been empirically examined cannot be made due to insufficient
understanding of the biological response to radiation exposure.
A theoretical model for the derivation of the D10-value has been proposed in this study to
mechanistically assess the production of radiation-induced DNA damage by energetic electrons.
The step-by-step Monte-Carlo simulation technique, which employs the detailed histories of the
ionizing particles and the radiolytic species, was utilized. The effects of selected parameters
including the genomic sequence, the type of DNA double strand break, the DNA damaging
agents, the radical scavengers, the degree of dispersion of DNA molecules, and the number of
genome equivalents were hypothetically investigated. The developed computational methodology
as well as the results presented can be used as an analytical tool to evaluate the impact of
ionizing radiation on cell survival.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-2009-05-351 |
| Date | 2009 May 1900 |
| Creators | Ekpanyaskun, Nont |
| Contributors | Moreira, Rosana |
| Source Sets | Texas A and M University |
| Language | English |
| Detected Language | English |
| Type | Book, Thesis, Electronic Dissertation, text |
| Format | application/pdf |
Page generated in 0.0023 seconds