Like the majority of host cell mRNAs, the mRNAs of influenza virus are capped and polyadenylated. The NS1 protein of influenza has been implicated as a translational activator for both influenza and reporter gene mRNAs. Data is presented showing that influenza A virus infection resulted in an increased ratio of cap-dependent to cap-independent translation. This ratio increase was largely due to an increase in cap-dependent translation. These experiments employed a bicistronic reporter construct measuring cap-dependent and cap-independent translation in a single sample. Expression of NS1 alone resulted in a small, but reproducible increase in the ratio of cap-dependent to cap-independent translation. Additionally, with use of an NS1 deleted mutant influenza A virus (delNS1) it is shown that infection without NS1 expression produced less of a translation ratio increase compared to wild-type virus infection. Furthermore, expression of NS1 rescued a more wild-type ratio increase in delNS1 infected Vero cells. These results implicate NS1 as playing a role in increasing the ratio of cap-dependent to cap-independent translation in influenza A virus infected cells. Additionally, eIF4E-binding protein-1 (4E-BP1), a member of the protein family that inhibits cap-dependent translation through their inhibition of the cap-binding protein, eukaryotic initiation factor 4E (eIF4E), is shown to be inactivated throughout the majority of the influenza A virus infection process.
| Identifer | oai:union.ndltd.org:uky.edu/oai:uknowledge.uky.edu:gradschool_diss-1480 |
| Date | 01 January 2004 |
| Creators | McCoy, Morgan Hager |
| Publisher | UKnowledge |
| Source Sets | University of Kentucky |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | University of Kentucky Doctoral Dissertations |
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