In this study, we have developed a novel way of generating and exposing biological organisms (both prokaryotic and eukaryotic) to silver nanoparticles (AgNPs) and studying the biochemical changes induced by these particles. We analyzed the various organs of Wistar rats for localization and quantification of these particles using mass spectrometric and molecular biological techniques. Highest levels of AgNP was found in the lung tissue in addition to being present in the liver and kidneys. Analysis of the of the blood plasma from AgNP exposed rats revealed elevated levels of glutathione-disulfide, which is indicative of reactive oxygen species (ROS) generation, which was further validated using ROS specific immunofluorescence staining of liver tissue. Quantification of blood lactate levels of the AgNP exposed rats showed increased lactate levels, which is indicative of anaerobic respiration and may result from AgNP-induced oxidative stress. Further analysis of bone marrow cells from AgNP exposed rats showed a higher number of micronuclei formation in developing erythrocytes and bone marrow cytotoxicity. Finally, analysis of the genes involved in the renin-angiotensin system (RAS) and inflammatory response revealed upregulation in transcript levels of many of these important genes in the liver tissue. Taken together, our study provides an initial road map for the identification of different signaling pathways that are altered by the AgNP exposure and contributes to a comprehensive understanding of the mechanism involved in silver nanoparticle-induced toxicity.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc1752347 |
| Date | 12 1900 |
| Creators | Nayek, Subhayu |
| Contributors | Verbeck, Guido, Lund, Amie K, Burggren, Warren W, Jagadeeswaran, Pudur, Fuchs, Jannon L |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | xi, 139 pages, Text |
| Rights | Public, Nayek, Subhayu, Copyright, Copyright is held by the author, unless otherwise noted. All rights Reserved. |
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