Considering that both cardiovascular disease (CVD) and congenital heart diseases (CHD) are still the leading cause of morbidity and mortality worldwide, there is a need for a robust and reliable cardiac model. Cardiac organoids are complex, three-dimensional cellular constructs that recapitulate the processes of the human embryonic heart. However, certain vital morphological features within the fetus are not yet replicable with cardiac organoids. Here we report our investigation to generate cardiac organoids with chamber formations. Our method involves modulating the Wnt pathway at two different instances while also implementing two cell seeding densities, all to determine the most optimized that to produce chamber formations within cardiac organoids.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc2179331 |
| Date | 07 1900 |
| Creators | Seddoh, Percyval Prince-Danny |
| Contributors | Yang, Huaxiao, Lee, Moo-Yeal, Ecker, Melanie |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | Text |
| Rights | Public, Seddoh, Percyval Prince-Danny, Copyright, Copyright is held by the author, unless otherwise noted. All rights Reserved. |
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