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Production, Location, and Binding of Violacein in Janthinobacterium

Violacein is a purple pigment typically produced by species of Chromobacterium and Janthinobacterium. A soil isolate, identified as Janthinobacterium, was studied. Maximal pigmentation occurred at 250C under aerobic conditions in the Keeble and Cross medium. Intracellular pigment was shown to be located in the cell membrane. Comparision of pigment production and growth curves indicated that violacein is synthesized in the cell and released into the environment possibly as a result of cell lysis. Extracellular pigment is water soluble, makes up 60% of the total pigment and shows a blue shift when compared to solvent extracted pigment. Results from purification indicated that the pigment is non-covalently bound to a small protein and aggregated into a larger molecule.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc798326
Date05 1900
CreatorsLin, Ying-Chuan
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatvii, 67 leaves : ill., Text
RightsPublic, Lin, Ying-Chuan, Copyright, Copyright is held by the author, unless otherwise noted. All rights

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