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A Comparison of Thermogenesis by Selected Substrates on Hypothermic Rat Liver

The thermogenic effects in hypothermia of four substrates--alanine, glycine, ethano, and pyruvate - were studied in seventeen experiments. Albino rats were decapitated, and their livers were removed. The livers were homogenized with phosphate buffer at -5° C. After equilibration in a refrigerated Warburg apparatus at 20° C, the substrates were added and tissue respiration was recorded over three hours. Heat production was calculated from O2 uptake and CO2 production. Results showed that alanine, glycine, and pyrvate yielded 93.19, 89.86, and 89.89 x 10^6 kg-cal compared to a control value of 86.11 x 10^-6 kg-cal. Ethanol provided 110.31 x 10^-6 kg-cal, a value significantly greater than for the other substrates. The substrates studied, especially ethanol, did, therefore increase heat production in an artificially hypothermic environment in homogenized rat livers.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc935582
Date08 1900
CreatorsLong, James T.
ContributorsRedden, David R., Norton, S. J., Harris, Ben G.
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatv, 25 leaves : ill., Text
RightsPublic, Long, James T., Copyright, Copyright is held by the author, unless otherwise noted. All rights

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