Background: Exosomes have great potential in regenerative medicine through the
transfer of their bioactive cargos, such as RNA. tRF RNA and tiRNA are tRNAderived
non-coding RNA. Here, we sought to identify the tRF/tiRNA profile in human
mesenchymal stem cell (hMSC) exosomes. Methods: Bone marrow hMSCs were
cultured with/without osteogenic differentiation medium and exosomes were
harvested. RNA was extracted from: 1) control cells (Cell-NT); 2) control exosomes
(EXO-NT); 3) differentiated cells (Cell-OM); 4) exosomes produced by differentiated
cells (EXO-OM). RNA was sequenced to profile the small RNA with a focus on
tRF/tiRNA. Results: tRF/tiRNA was highly enriched in hMSC exosomes. Less
diversity was seen in the tRF/tiRNA profile in exosomes than that in parent cells.
Selective tRF/tiRNA were packed into MSC exosomes and their profile is dependent
on the cell maturation status. Conclusions: Our results suggest that tRF/tiRNA may
play a role in mediating the function of exosomes in tissue regeneration.
| Identifer | oai:union.ndltd.org:vcu.edu/oai:scholarscompass.vcu.edu:etd-6722 |
| Date | 01 January 2018 |
| Creators | San, Khin MiMi |
| Publisher | VCU Scholars Compass |
| Source Sets | Virginia Commonwealth University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
| Rights | © The Author |
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