Amphibians worldwide are declining, increasing the demand for monitoring populations of many threatened amphibians, including the South African Critically Endangered micro frog (Microbatrachella capensis). I attempted to improve ongoing monitoring efforts by determining the calling ecology of the micro frog population on the Cape Flats. I used acoustic spatial capture recapture to calculate call density and identify the main factors that determine periods of maximum frog calls. Increased calling behaviour was found early in the season and in response to rainfall. In addition, micro frogs were found to call more at night than during the day. This suggests that future monitoring of this species should occur at night, after rain, and early in the winter breeding season. From the call density estimates, I calculated the size of the micro frog population on the Cape Flats to be about 200 adult frogs when an equal sex ratio of adult males and females is assumed. Future monitoring and conservation efforts should take into consideration this baseline population estimate and keep track of any demographic trends in the population.

The micro frog, Microbatrachella capensis (Boulenger, 1910), is a Critically Endangered anuran found in fragmented marshland habitats along the southern coast of the Western Cape Province of South Africa. The genetic diversity between and within the remaining populations (Kenilworth, Grootwitvlei, Kleinmond, Lamloch, Hagelkraal, and Buffeljacht/Ratelrivier) of Microbatrachella capensis in the Western Cape Province of South Africa was assessed, sampling 12 specimens from each population. Genetic diversity was determined from an analysis of mitochondrial DNA sequences obtained from the ND2 gene region/locus. A phylogeographic analysis of the populations of micro frogs sampled was used to determine the current genetic structure and the history of the species. The analysis divided the six populations of the species into two lineages. The variation within the populations ranged from two to six haplotypes per population. Nested clade analysis inferred allopatric fragmentation for three out of the five significant clades. The division of the lineages into two geographical units, the absence of gene flow and the allopatric fragmentation indicates long-term isolation, around 1.09-1.52 my ago. The isolation and fragmentation of the populations is postulated to be due to historical sea level fluctuations that occurred in Southern Africa during the Quaternary Period. Allopatric fragmentation and lack of gene flow among populations within the two major lineages are due to recent habitat destruction through development. The recognition that the species contains two evolutionarily significant units, corresponding to Agulhas and western lineages, will aid future conservation efforts to save this species. / Dissertation (MInstAgrar)--University of Pretoria, 2005. / Zoology and Entomology / unrestricted