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Aspects of molecular analysis in myeloproliferative disorders and myelodysplastic syndromesChampion-Suntharalingam, K. M. January 2001 (has links)
No description available.
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162 |
Cloning, mutagenesis and expression studies of the pazS gene, encoding pseudoazurin from Paracoccus denitrificansPearson, Isobel V. January 1999 (has links)
No description available.
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163 |
22q11 deletion : frequency, predictive value and implication for clinical practiceLiLing, Jesse January 2000 (has links)
No description available.
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164 |
Characterisation of a novel multi-tissue tumour suppressor gene in mouseO'Neill, Vincent John January 2001 (has links)
No description available.
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165 |
Physical and genetic characterisation of the CLS gene region on human Xp22.13Bird, Helen January 1997 (has links)
No description available.
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166 |
Investigating pathological mutations in the neurofibromatosis type 2 tumour suppressor geneMason, Susan January 1998 (has links)
No description available.
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167 |
Characterisation of the murine homologue of the CIC-5 gene : a voltage-gated chloride channel implicated in human X-linked hereditary nephrolithiasisTanaka, Karo January 1999 (has links)
No description available.
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168 |
Analysis of the Nucleioprotein Complexes Essential for P1 Plasmid PartitionVecchiarelli, Anthony 01 September 2010 (has links)
For all organisms, segregation and proper intracellular localization of DNA are essential processes in ensuring faithful inheritance of genetic material. In prokaryotes, several different mechanisms have developed for efficiently moving chromosomal DNA to proper cellular locations prior to cell division, and the same holds true for bacterial plasmids. Low-copy-number plasmids and bacterial chromosomes encode active partition systems to ensure their inheritance within a bacterial cell population. One of the well-studied models of partition is that of the P1 plasmid in E. coli. The partition system encoded by the P1 plasmid is known as parABS - ParA is the partition ATPase, ParB is the partition site binding protein and parS is the partition site. The goal of this thesis was to investigate the nucleoprotein complexes essential in the P1 plasmid partition reaction. First, I examined how a single ParB dimer can bind its complicated arrangement of recognition motifs in parS to initiate the partition reaction. I then characterized a novel ParA interaction with the host nucleoid that is critical for proper P1 plasmid dynamics in vivo. Finally, I demonstrate how ParA can act as an adaptor between the nucleoid and the partition complex; effectively allowing the plasmid to use the nucleoid as a track for its intracellular movement and localization. My thesis work provides evidence towards a model that explains the P1 plasmid partition mechanism.
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Characterisation and attempted cloning of the hfaB gene of Aspergillus nidulansBarnett, Deborah Amanda January 1996 (has links)
No description available.
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170 |
Regulation of chromosome segregation by Shugoshin and protein phosphatase 2A in budding yeastClift, Dean January 2010 (has links)
The accurate distribution of genetic information (chromosomes) during cell division is essential for the growth and proliferation of all living organisms. Errors in chromosome segregation in humans have been linked to cancer progression, infertility and developmental diseases. In my PhD I study how chromosome segregation is regulated in the genetically amenable budding yeast Saccharomyces cerevisiae. Since the mechanisms of chromosome segregation are highly conserved amongst eukaryotes, studies in yeast will provide a fundamental understanding of this process. Sgo1 is the budding yeast member of a highly conserved family of shugoshin proteins, which play a key role in chromosome segregation. My work characterizes a previously unidentified role of Sgo1 in inhibiting separase; an enzyme that triggers chromosome segregation by cleaving the cohesin protein complex that holds replicated chromosomes together. I demonstrate that this novel function of Sgo1 requires a specific form of Protein Phosphatase 2A (PP2ACdc55), an enzyme that itself is highly conserved amongst eukaryotes. I propose that PP2ACdc55 is a separase inhibitor that is employed by Sgo1 when sister chromatids are not under tension. Finally, I go on to initiate preliminary studies into the mechanism whereby PP2ACdc55 inhibits separase. In sum, this study uncovers an additional layer of separase regulation mediated by Sgo1 and PP2ACdc55 and therefore makes a significant contribution to our understanding of the all-or-nothing nature of chromosome segregation.
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