Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-10-04T21:38:34Z No. of bitstreams: 1 Disserta??o - D?bora Marchesine. vers?o final.pdf: 1771951 bytes, checksum: 203519d3cb711fb7c54beb6fc48672cb (MD5) / Made available in DSpace on 2017-10-04T21:38:34Z (GMT). No. of bitstreams: 1 Disserta??o - D?bora Marchesine. vers?o final.pdf: 1771951 bytes, checksum: 203519d3cb711fb7c54beb6fc48672cb (MD5) Previous issue date: 2017-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Aromatic plants of the genus Lippia (Verbenaceae) are widely used in traditional medicine. A number of Lippia species can be found in the Caatinga biome in Northeastern Brazil, such as Lippia bromelyana Moldenke, endemic to the state of Bahia. This study investigated the chemical composition, acute toxicity, antinociceptive and anti-inflammatory effect of the leaf essential oil of L. bromelyana (EOLB) in mice.The chemical composition of EOLB, assessed by gas chromatography with flame ionization detection (GC/FID) and gas chromatography-mass spectrometry (GC/MS), detected limonene (29.90%) and piperitenone oxide (26.91%) as major compounds. Intraperitoneal administration of 300 mg/kg of EOLB caused no acute toxic effects or deaths in the mice. At doses of 75, 150 and 300 mg/kg caused no changes in their motor activity in the rotarod test, but promoted a significant antinociceptive effect on abdominal contortions in the acetic acid-induced constriction test, in both phases of the formalin test, glutamate or cinnamaldehyde-induced nociception tests and the Randall-Selitto test. In the hot plate test, EOLB (150 or 300 mg/kg) increased latency at 30, 60 and 120 min after administration; at a dose of 300 mg/kg, the action reversed by naloxone suggests participation of the opioid pathway. Moreover, EOLB (75, 150 or 300 mg/kg) significantly reduced the inflammatory effect of carrageenan in the paw edema test. Thus, these results suggest that EOLB promotes antinociceptive and anti-inflammatory activity by central and peripheral action. / Plantas arom?ticas do g?nero Lippia (Verbenaceae) s?o muito utilizadas pela medicina tradicional no tratamento de dist?rbios relacionados ao sistema respirat?rio e a problemas gastrointestinais. Algumas esp?cies de Lippia podem ser encontradas na caatinga, localizado na regi?o Nordeste do Brasil. Dentre essas esp?cies Lippia bromelyana Moldenke, ? end?mica do estado da Bahia. Este trabalho procurou investigar a composi??o qu?mica, o efeito t?xico agudo, antinociceptivo e anti-inflamat?rio do ?leo essencial das folhas de L. bromelyana (OELB) em camundongos. A composi??o qu?mica do OELB avaliada por CG/EM e CG/DIC, detectou limoneno (29,90%) e ?xido de piperitenona (26,91%) como compostos majorit?rios. A administra??o intraperitoneal do OELB na dose 300 mg/kg n?o causou efeitos t?xicos agudos nem mortes nos camundongos. Nas doses 75, 150 ou 300 mg/kg OELB n?o causou altera??es na atividade motora dos camundongos no teste do rota-rod, mas promoveu significante efeito antinociceptivo no teste de contor??es abdominais induzidas por ?cido ac?tico, em ambas as fases do teste da formalina, nos testes de nocicep??o induzida por glutamato ou cinamalde?do e no teste de Randall-Selitto. No teste da placa quente, OELB (150 ou 300 mg/kg) provocou o aumento na lat?ncia aos 30, 60 e 120 min ap?s sua administra??o; na dose 300 mg/kg a a??o do OELB revertida pela naloxona, sugere a participa??o da via opi?ide. Al?m disso, OELB (75, 150 ou 300 mg/kg) reduziu significativamente o efeito inflamat?rio da carragenina no teste de edema de pata. Esses resultados sugerem, portanto, que OELB promove a??o antinociceptiva e anti-inflamatoria por a??o central e perif?rica.