Sun Qiang. / "December 1999." / Thesis (Ph.D.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (p. 119-139). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web.

With the aim to enhance the efficiency of utilization of crystalline methionine supplemented in the high plant-protein diet for grouper (Epinephelus coioides), this study used Bacillus subtitlis spore as a probiotic additive in the diet to shorten the absorption time difference between protein-bound amino acid and crystalline methionine. The study was conducted in two parts. In the first part, juvenile groupers were fed for 14 weeks with 5 experimental diets: fish meal diet, high plant-protein diet with/without crystalline methionine, as well as with/without B. subtitlis spore separately. Growth performance, PER, protein digestibility, amino acid digestibility, non-specific immune responses, and free amino acid concentration in both muscle and serum were assessed. The second part was a time-series study on serum free amino acids concentration after a force-feeding experiment. The results showed that crystalline methionine supplementation in the high plant-protein diet effectively improved the growth of E. coioides (P<0.05). However, B. subtitlis spore supplementation did not affect fish growth performance significantly (P>0.05). A delay in the appearance of peak serum amino acid concentration was observed when fishmeal was partially replaced by soy protein. On the other hand, the force-feeding experiment showed that serum essential amino acid (include methionine) concentrations droped drastically after they reached the peak concentrations from being forced-fed with the B. subtilis containing diet. Supplementation of crystalline methionine seemed to ease the drop of serum methionine concentration. Based on these results, it is concluded that addition of B. subtitlis spore in high plant-protein diet for the grouper does not enhance the utilization of crystalline methionine, but supplementation of crystalline methionine significantly improve the growth performance of the grouper.

Diss. (sammanfattning) Stockholm : Karol. inst., 2005. / Härtill 5 uppsatser.

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.

Thesis (Ph.D.)--University of Florida, 2004. / Typescript. Title from title page of source document. Document formatted into pages; contains 210 pages. Includes Vita. Includes bibliographical references.

Made available in DSpace on 2014-06-11T19:27:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-25Bitstream added on 2014-06-13T20:36:07Z : No. of bitstreams: 1 domingues_chf_me_jabo.pdf: 336764 bytes, checksum: 96a84ad26e208c18fbcdc65dde25a4fe (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O presente estudo teve por objetivo avaliar o efeito do uso de diferentes níveis de lisina e de metionina + cistina digestíveis durante o período pós - muda, sobre a recuperação corporal, desempenho, qualidade de ovos e morfometria do aparelho reprodutor, fígado e pâncreas de poedeiras comerciais no segundo ciclo de produção. Foram utilizadas 432 poedeiras comerciais da linhagem Isa Brown, com 72 semanas de idade, distribuídas em 54 parcelas, em um delineamento inteiramente casualizado com seis tratamentos e nove repetições de oito aves cada. Durante o descanso foram utilizados seis rações cujos níveis de lisina e metionina + cistina digestíveis variaram: 0,48% de lisina digestível e 0,43% de metionina+cistina digestíveis; 0,48% de lisina digestível e 0,47% de metionina+cistina digestíveis; 0,48% de lisina digestível e 0,52% de metionina+cistina digestíveis; 0,56% de lisina digestível e 0,50% de metionina+cistina digestíveis; 0,56% de lisina digestível e 0,56% de metionina+cistina digestíveis; 0,56% de lisina digestível e 0,62% de metionina+cistina digestíveis.Os dados obtidos foram submetidos à análise de variância e em caso de efeito significativo, a comparação de médias foi realizada a 5% de probabilidade através do teste de Tukey. Os diferentes níveis de lisina e de metionina+cistina digestíveis das dietas de descanso, determinaram efeitos significativos sobre os parâmetros de desempenho das aves. Observou-se que, o nível de 0,56% de lisina e 0,56% de metionina + cistina digestíveis, proporcionou maior peso dos ovos durante o segundo ciclo de produção / This study was conducted to evaluate the effect of using different levels of lysine and methionine + cystine, about the body recovery, performance and egg quality of laying hens in the post molt. It was used four hundred and thirty two hens of Isa Brown strain, with 72 weeks of age, distributed in 54 cages in a completely randomized design with six treatments and nine replicates of eight birds each. During the rest period, were used six diets with different levels of digestible lysine and methionine + cystine. The values ranged from: 0.48% digestible lysine and 0,43% methionine + cystine; 0.48% digestible lysine and 0.47% methionine + cystine; 0.48% digestible lysine and 0.52% methionine + cystine; 0.56% digestible lysine and 0.50% methionine + cystine; 0.56% digestible lysine and 0, 56% methionine + cystine; 0.56% digestible lysine and 0.62% methionine + cystine. The data were subjected to analysis of variance and in case of significant effect, the comparison of means was performed at 5% probability by Tukey test. The different levels of lysine and methionine + cystine diets of rest have determined significant effects on the performance parameters of laying hens. It was observed that the level of 0.56% lysine and 0.56% methionine + cystine, resulted in greater weight of eggs during the second production cycle

Leukemia is the most common cancer of children, moreover it is also not uncommon of elderly patients. Research has focused on the development of specific antileukemic drugs in recent years. Abnormalities in tumor cell metabolism that can be targeted during treatment appear to be the key. Natural 1,4-naphthoquinones, including quambalarin B produced as a secondary metabolite by the basidiomycetes of Quambalaria cyanescens, are known for their therapeutic effects. Not surprisingly, Quambalarine B has also been shown to inhibit cell proliferation in some leukemic cell lines and subsequently caused cell death. In the present thesis, I tried to observe changes in amino acid metabolism by monitoring amino acid levels in the intracellular and extracellular environment of leukemic cells after treatment with Quambalarine B using amino acid analysis with fluorescence detection. The observation was performed in Jurkat, Ramos and THP-1 cell lines, each of these lines represents another type of leukemic disease. [IN CZECH] Key words Amino acid analysis, amino acid metabolism, Quambalarine B, leukemia

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第18331号 / 農博第2056号 / 新制||農||1022(附属図書館) / 学位論文||H26||N4838(農学部図書室) / 31189 / 京都大学大学院農学研究科応用生命科学専攻 / (主査)教授 植田 充美, 教授 三上 文三, 教授 小川 順 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

Amino acids constitute the major organic form of transported nitrogen in plants, elements for protein synthesis, and precursors of many plant secondary metabolites, such as lignin, hormones, and flavonoids. Furthermore, amino acid metabolism lies at the crossroad of carbon and nitrogen metabolism. The Arabidopsis glutamine dumper1 (gdu1) mutant secretes glutamine from hydathodes, a phenotype caused by the overexpression of Glutamine Dumper1 (GDU1). GDU1 is a small transmembrane protein presents only in higher plants. The gdu1-1D mutant shows a pleiotropic phenotype: perturbed amino acid metabolism, tolerance to exogenous toxic concentrations of amino acids, elevated amino acid export, and activated stress/defense responses, lesions, and smaller rosettes. The biochemical function of GDU1 remains elusive. To better elucidate the biological processes leading to the complex Gdu1D phenotype, two approaches were conducted: (1) An ethyl methanesulfonate suppressor screening of the Gdu1D phenotype, which led to the isolation of intragenic mutations in GDU1 and mutations in the ubiquitin ligase LOG2 (Loss Of Gdu1D 2). Study of the intragenic mutations in GDU1 helped to characterize its structure-function relationships. Characterization of LOG2 showed that LOG2 interacts with GDU1 and is necessary for the Gdu1D phenotype. (2) The responses of the plant to the dexamethasone-induced expression of GDU1 were studied over time. This experiment identified major signaling pathways contributing to different components of the Gdu1D phenotype and the early events triggered by the perturbation of amino acid homeostasis. Our results showed that GDU1 overexpression first increases amino acid export, which is followed by amino acid imbalance and stress responses. This study sheds light on how amino acid imbalance interacts with various plant signaling pathways and stress responses, and suggests that LOG2 is involved in this process. / Ph. D.