S. chartarum spores are prevalent in buildings affected by flooding and have been tied to occurrences of pulmonary hemosiderosis in infants in Cleveland, Ohio, several years ago. Rationale: Past studies have focused on the effects of S. chartarum spores on surfactant in terms of composition and quantity and very little has been done to investigate the effects of S. chartarum extracts on surfactant protein expression in the fetal lung. Methods: Spore extracts were incubated with cultures of human lung A549 cells, fetal rat lung type II and fibroblast cells. MTT formazan assays analyzed cell viability. The expression of surfactant protein A and surfactant protein B was assessed using western blotting results. Captive bubble surfactometry investigated the quality of surfactant in terms of surface tension and bubble volume. Electron microscopy examined changes in cellular structure of treated cells. Conclusions: Exposure to S. chartarum extracts had negative effects on cell viability, expression of surfactant proteins A and B and the production and quality of pulmonary surfactant. Spore associated changes were apparent in the lamellar bodies of fetal rat lung alveolar type II, and human A549 cells.

Condensation, of steam bubbles generated at an orifice and rising freely through water, subcooled from 5 K to 36.6 K at pressures of 1 bar and 2 bar, has been analysed theoretically and experimentally. Orifice diameters were 1 mm and 2 mm, and steam flow rates of 0.5, 1 and 1.5 g/min were used. The data indicate a decrease in collapse Fourier number with increase in either Jakob number or steam flow rate, or with a decrease in pressure, while change in orifice diameter does not have a significant effect on collapse Fourier number. Average values of heat transfer coefficient around the collapsing bubbles have been determined to be between 0.15 . 10⁵ - 0.35 . 10·⁵ W/m·²K. The effect of bubble distortion and of local heating of the liquid, close to the orifice, due to condensation of the bubbles, have both been included in the quasi-steady state theory which has been presented. The experimental data is compared with the theoretical predictions. A semi-empirical correlation for bubble rise height has been proposed, which is also based on the quasi-steady theory combined with a correlation for the velocity of steam bubbles condensing in subcooled water.

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S. chartarum spores are prevalent in buildings affected by flooding and have been tied to occurrences of pulmonary hemosiderosis in infants in Cleveland, Ohio, several years ago. Rationale: Past studies have focused on the effects of S. chartarum spores on surfactant in terms of composition and quantity and very little has been done to investigate the effects of S. chartarum extracts on surfactant protein expression in the fetal lung. Methods: Spore extracts were incubated with cultures of human lung A549 cells, fetal rat lung type II and fibroblast cells. MTT formazan assays analyzed cell viability. The expression of surfactant protein A and surfactant protein B was assessed using western blotting results. Captive bubble surfactometry investigated the quality of surfactant in terms of surface tension and bubble volume. Electron microscopy examined changes in cellular structure of treated cells. Conclusions: Exposure to S. chartarum extracts had negative effects on cell viability, expression of surfactant proteins A and B and the production and quality of pulmonary surfactant. Spore associated changes were apparent in the lamellar bodies of fetal rat lung alveolar type II, and human A549 cells.

In this thesis study, air bubble motion in counter-current water flow conditions in a vertical pipe is investigated experimentally. For this purpose, a test set-up was designed and constructed. Images of motions of single bubbles, having different diameters in the range of 3.0-4.8 mm, generated by specially designed bubble injectors were recorded by using a monochrome camera, an image capture card and a PC. Recorded video images were processed to obtain the necessary data for the The purpose of the study is to determine variation as a function of the equivalent bubble diameter, water flow velocity and related dimensionless numbers / Reynolds, Re / E&ouml / tv&ouml / s, Eo / and Weber, We, and is to investigate the bubble shapes and bubble travel paths. Bubble behaviour was investigated at six different counter-current water flow velocities (6.5 cm/s, 7.9 cm/s, 10.5 cm/s, 12.9 cm/s, 15.4 cm/s, and 18.2 cm/s) in addition to stagnant water condition which is taken as the reference case. The direction of the bubble motion is upwards and the direction of the water flow is downwards (i.e. counter-current). Distilled water was used in the experiments. The results of this thesis study for the stagnant water condition have shown good consistency with the previous theoretical and experimental studies found in the literature. For the studied range of bubble diameters, it is observed that the bubble average relative velocity for a certain bubble diameter is less under counter-current water flow conditions than that under stagnant water condition and the drag coefficient values for a certain bubble diameter is higher under counter-current water flow conditions than those under stagnant water condition.

Thesis--University of California, Berkeley, 1961. / "UC-34 Physics" -t.p. "TID-4500 (16th Ed.)" -t.p. Includes bibliographical references (p. 74-76).

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