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Förekommer Stafylococcus aureus, Stafylococcus epidermidis, Escherichia coli, Streptococcus pyogenes och Bacillus sp : på allmänna ytor på avdelningen SET (ekonomi och teknik), Halmstad högskola?Restedt, Malou, Ala-Mikkula, Pia January 2007 (has links)
Stafylococcus aureus, Stafylococcus epidermidis, Escherichia coli, Streptococcus pyogenes och Bacillus sp. är bakterier som förekommer i vår normalflora eller runt om i vår omgivning. De är i de allra flesta fall harmlösa men kan orsaka sjukdom ifall de hamnar i t.ex. sår eller kontaminerar mat. Vissa av dessa bakterier har även en tendens att utveckla resistens mot antibiotika, infektioner orsakade av resistenta bakterier kan bli allvarliga och väldigt svåra att behandla. Detta är det främsta skälet till varför vi har valt att undersöka ifall de ovan nämnda bakterierna förkommer på avdelningen SET på Halmstad högskola. För att kunna identifiera de bakterier vi fann använde vi oss av en rad olika biokemiska tester och resistensbestämningen utfördes bl.a. med hjälp av en PCR körning. Vi fann alla de bakterier vi sökte efter förutom S. pyogenes och ingen av de funna visade på någon resistens mot antibiotika.
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The function and structural characteristics of conserved regions within Escherichia Coli small subunit ribosomal RNAAlmehdi, Mirza A. 10 September 1991 (has links)
Ribosomes are multicomponent macromolecular particles and
are essential for the survival of cells in all organisms. The ribosome's
universal function is to catalyze polypeptide synthesis through
translation of mRNA transcripts. Ribosomes from Escherichia coli,
eubacterial organisms, have a sedimentation coefficient of 70S and are
composed of 30S and 50S ribonucleoprotein subunits. The small
ribosomal subunit is an assembly of 21 different proteins and a 16S
ribosomal RNA. Within the 16S rRNA there are a few short stretches of
universally conserved sequences spanning positions 517-533, 1394-1408,
and 1492-1506. Clear functions for these sequence zones have not yet
been assigned.
Here I report a kinetic analysis of these highly conserved regions
in the 16S rRNA and within the 30S ribosomal subunits. Binding affinity
was measured in experiments that were based on protection from
nuclease 51 digestion of short oligodeoxynucleotides hybridized to the
designated regions. DNAs hybridized to regions 1400 and 1500 show
significant differences in the apparent dissociation constants when
measured in 30S particles as opposed to those found for 16S rRNA.
Region 525 showed no difference in kinetic behavior.
To further elucidate the functional and structural role played by
the region centered about C1400 in 16S rRNA, a four nucleotide deletion
was constructed within this region. The deletion was introduced by
direct RNA manipulation using DNA/RNA hybridization, RNase H
digestions, and ligation of the correct RNA fragments with T4 RNA
ligase. I improved ligation efficiency of large RNA molecules by
including a connector looped short DNA oligomer. Recycling products
through phenyl boronate agarose (PBA-30) column also improved the
efficiency of ligation.
The mutagenized 16S rRNA fully reassembles into 30 particles
and the altered 30S subunit possesses all of the normal ribosomal
proteins. Altered ribosomes were functional in in vitro translation of
MS2 mRNA. The altered ribosomes have lower translational activity
relative to controls. Here I present indirect evidence suggesting that the
decrease in the synthesis of MS2 coat proteins is the result of premature
termination.
The altered 16S RNA in ribosomes had an apparent dissociation
constants with DNA probes comparable to those found for normal 16S
rRNA. This suggest that the RNA is less flexible in the particle relative
to normal 30S subunits. The deletion at 1400 did not have any effect on
the physical properties of the 1500 region, as measured by DNA
hybridization. A minor, but significant, effect on the 525 region was
observed. A possible RNA/RNA interaction within the 30S particle is
proposed to account for this observation. / Graduation date: 1992
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Swarming in Salmonella typhimurium and Escherichia coli /Toguchi, Adam James Masao, January 1999 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 1999. / Vita. Includes bibliographical references (leaves 149-164). Available also in a digital version from Dissertation Abstracts.
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Formation de biofilms par Escherichia coli K-12 rôle des systèmes à deux composants dans la synthèse des curli /Jubelin, Grégory Dorel, Corinne January 2005 (has links)
Thèse doctorat : Ecologie Microbienne : Villeurbanne, INSA : 2005. / Titre provenant de l'écran-titre. Bibliogr. p. 107-121.
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Optimisation de la recherche des Escherichia coli producteurs de Shiga-toxines (STEC)Vimont, Antoine Delignette-Muller, Marie-Laure Vernozy-Rozand, Christine January 2007 (has links) (PDF)
Reproduction de : Thèse de doctorat : Écologie microbienne : Lyon 1 : 2007. / Titre provenant de l'écran titre. 335 réf. bibliogr.
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F exclusion of bacteriophage T7Cheng, Xiaogang. January 2002 (has links) (PDF)
Thesis (Ph. D.)--University of Texas at Austin, 2002. / Vita. Includes bibliographical references. Available also from UMI Company.
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Anchored periplasmic expression (APEx) a versatile technology for the flow cytometric selection of high affinity antibodies from Escherichia coli expressed libraries /Harvey, Barrett Rowland, Georgiou, George, January 2003 (has links) (PDF)
Thesis (Ph. D.)--University of Texas at Austin, 2003. / Supervisor: George Georgiou. Vita. Includes bibliographical references. Available also from UMI Company.
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Symmetry and stalk architecture in the E. coli F₁ ATPase /Hausrath, Andrew Clark, January 2000 (has links)
Thesis (Ph. D.)--University of Oregon, 2000. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 87-93). Also available for download via the World Wide Web; free to University of Oregon users.
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Heterologous expression and localization of cryptic haloacid dehalogenase Chd1 of Burkholderia cepacia MBA4 /Sze, Johnny. January 2001 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2002. / Includes bibliographical references (leaves 145-174).
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Cloning, characterization and sequence determination of a cellobiase gene from Cellulomonas Biazotea in Escherichia coli /Lam, Yuen Yan. January 2003 (has links)
Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2003. / Includes bibliographical references (leaves 102-112). Also available in electronic version. Access restricted to campus users.
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