Perineal descent (PD) is a sign of connective tissue weakness of the pelvic floor, it can be measured mechanically or radiologically. Joint hypermobility can be a sign of a generalised connective tissue abnormality, there is an increased incidence of pelvic organ prolapse and faecal incontinence amongst patients with heritable connective tissues diseases. To explore the relevance of PD and the relationship between connective tissue abnormality and pelvic floor dysfunction five studies were performed.A new mechanical device for the measurement of PD, the laser commode, and the established mechanical device, the perineometer were compared to the current gold standard method of measurement, defaecating proctography in 68 subjects. The laser commode provided a mean overall PD measurement closer to that of proctography than the perineometer but the repeatability and reproducibility of the measurements were not accurate enough for the laser commode to be used either in the subsequent parts of this research project or in a clinical setting.Perineal descent was measured using proctography and joint hypermobility was measured using the Beighton score in 70 females with pelvic floor dysfunction. No correlation was found between PD and joint mobility.A review of 323 proctograms of females with pelvic floor dysfunction found an association between PD and rectal prolapse but no association between either PD and rectocele formation or PD and rectal intussusception. The Pelvic Floor Distress Inventory questionnaires of 133 females were correlated with their proctography findings. There was no association between PD and any of the clinical symptoms. Biopsies from the rectus sheath and pelvic floor fascia of 19 females with rectal prolapse were compared to those of 8 normal controls. There was no difference in collagen or elastin content between the groups but participant numbers were small. The pelvic floor fascia of the rectal prolapse group showed a higher percentage of well organised elastin than that of the control group but this did not reach statistical significance. Perineal descent does not appear to be a consistent indicator of severe pelvic floor connective tissue abnormality or injury. This study has furthered our understanding of perineal descent and the relationships between this finding and other pelvic floor disorders caused by connective tissue weakness. Future work will focus on further histological analysis of tissue from patients with rectal prolapse in combination with the use of more sensitive methods to establish the presence of an underlying connective tissue abnormality.

The thesis is focused on analysis of damage extent of dry floors in case of a flood. In the first part it deals with problems of buildings´ stress factors in the point of view of building physics, floor composition and its connection to constructions of timber houses. In the second part it describes testing of three experimental floor samples during simulated flood. It examines and evaluates the behavior and the way of spreading of the leaking water and the effect of moisture on the materials.

In U.S. swine herds, sow removal rates due to death and voluntary and involuntary culling exceed 50% annually. This loss poses an economic problem for producers because the cost of acquiring replacement females is great. Although research has shown that crowding in the nursery has negative impacts on growth, research describing effects of crowding on subsequent reproductive performance and longevity in sows is lacking. This experiment was conducted to determine the impacts of crowding during the nursery phase of production on growth, physiology, and immunology in replacement gilts. Gilts (22.3 ± 3.2 d of age and 5.6 ± 0.6 kg BW) were subjected to floor space allocations of 0.15, 0.19, or 0.27 m2/pig during a 7-wk nursery period. Floor space allocations were achieved by altering the number of pigs per pen (14, 11, and 8 gilts/pen, respectively). As was expected, reduced floor space allowance in the nursery negatively affected growth performance although there was inconclusive physiological and immunological evidence to suggest that pigs were experiencing highly stressful conditions. Although feed intake was not measured, changes in blood counts and blood chemistry for gilts allowed reduced floor space were similar to other studies that reported negative effects of crowding on feed consumption. Further study of the gilts involved in this study will aim to determine if there are any links between the effects of crowding during the nursery and subsequent reproductive performance and longevity in the breeding herd. / Master of Science

Regeneration of forest stands is often complicated by the establishment and competition of brush on logged and burned-over areas. The encroachment of brush in the ponderosa pine type of Idaho, particularly Ceanothus velutinus, Dougl. presents a difficult problem to the forest manager. The brush competition severely reduces the growth rate of associated trees, thus extending the period necessary to grow a crop of trees. This situation may seriously limit the economic production of a wood crop of ponderosa pine. Any approach to the solution of brush problems should include a thorough understanding of brush ecology. Those phases of ecology dealing with reproduction and distribution of brush need special emphasis. It has been assumed that most of the brush on logged or burned-over areas originated from seed stored in the forest floor; yet little is known of the quantity or distribution of these seeds. This study was carried out to learn what kind, number and variability of seed is present in the forest floor, and to investigate the number of seed located in different forest conditions, aspects and soil layers. To achieve the above objectives, 48 square-foot, duff-soil samples were taken in the ponderosa pine type of the Boise Basin Experimental Forest in Idaho. Seeds were separated from the samples and major species identified, pretreated and germinated. Appropriate statistical techniques were employed to determine significant results among the different forest conditions, aspects and soil layers sampled.

Strength and tone of the pelvic floor muscles are thought to play an important role in pelvic floor disorders, and are key outcomes monitored in pelvic floor rehabilitation, yet the standard approach to measuring these outcomes is through subjective assessment using manual palpation. While intravaginal dynamometers (IVD) have been designed to measure these characteristics in research settings, most are not sufficient to withstand the rigors of clinical use. This work presents the bench validation and subsequent updated design of a new mechanical constant speed mechanism for future inclusion in an IVD. Opening speeds of the original mechanism were validated with and without external loading using video analysis. The bench validation showed that the speed of arm opening was lower than the ranges specified for clinical use and was influenced by external loading. The mechanism was updated, and the bench test was repeated. The updated mechanism was found to provide output speeds that are within the ranges required clinically and were minimally impacted by external loading. The next step of this work is to reduce the size of the mechanism and improve output speeds to allow it to be assembled into a clinical prototype IVD.

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Forest plantations in the southeastern U.S. are fertilized to increase growth on infertile, sandy soils. Nitrogen (N) and phosphorus (P) are the most common growth limiting nutrients. A key question that arises following fertilization of these soils is whether the applied fertilizer benefits only the current trees in the stand or also improves long-term site quality. The objectives of this study were to compare accumulation of N and P in the forest floor and mineral soil among unfertilized and fertilized plantations, determine soluble and residual N and P fractions and soluble carbohydrate and phenol fractions in foliage and litter, determine whether higher N in the forest floor from fertilization resulted in increased release of N from the forest floor and increased mineral soil N availability, and determine loblolly pine forest floor decomposition rate and release of nutrients in a simulated disturbance environment. Research was conducted at a 25-year old loblolly pine (Pinus taeda L.) plantation in NC (SETRES) and 13-year old loblolly pine and sweetgum (Liquidambar styraciflua L.) plantations in GA (Mt. Pleasant). Fertilization resulted in increases in mineral soil N that were likely to be temporary and not sustained following cessation of fertilization N applications. This was likely due to an inability of acidic, sandy mineral soils to retain NH4-N and NO3-N. The forest floor accumulated N due to slow release of N during decomposition. Fertilization with N results in only temporary increases in mineral soil N availability that occur during fertilizer application and from forest floor decomposition. Future changes in N availability are primarily determined by decomposition of the forest floor following a disturbance that accelerates decomposition. In contrast to N, fertilization of loblolly pine and sweetgum with P results in a long-term increase in site P availability. Fertilization with P has lasting effects by increasing mineral soil P in stable forms that can be made available for plant uptake over time suggesting increased supply of P to trees in the next rotation. Retention of P in the mineral soil was likely due to the tendency of acidic, sandy mineral soils to accumulate P in Al- and Fe-phosphates. / Ph. D.