Dietary cyclopropenoid fatty acids (CPFA) have long been known to cause pronounced physiological disorders in both farm and laboratory animals. Past work has shown CPFA to be a powerful promoter of carcinogenesis in trout, rats and mice. Sterculic and malvalic acids (CPFA's) are found in seed lipids of plants from the order Malvales. Two members of this order are cotton and kapok, both of which are used extensively as cooking oils for human consumption. Present chemical and instrumental methods of analysis for CPFA are effective only at CPFA levels above 0.1%, and accurate only at levels above 1%. A more sensitive method of analysis was developed exploiting recent technological advances in glass capillary gas chromatography (GCGC). By the use of cold on-column injection, and positioning of the column at the base of the detector flame, this method eliminates two problem areas found in other GC methods. The other common component involved in CPFA decomposition, during GC analysis, is the column. Vitrious silica columns with an inert stationary phase, SE-30, were shown to separate the highly reactive CPFA without decomposition. Proof of stability during analysis was obtained by changing the variables of relative time on column and column temperature. This method not only allows individual determination of sterculic and malvalic acid concentrations, it is rapid, accurate (to the 70 parts per million range), and is superior to other instrumental and chemical methods. The CPFA concentration for okra, hollyhock, cheese weed, seashore mallow, kapok, and white cap cottonseed oil are as follows: 0.3% to 0.92%, 0.33%, 2.6%, 2.6%, 12.8%, and 75ppm, respectively. The two different values for okra were found because seeds from two different growing seasons were analyzed. No CPFA could be detected in Diet Imperial Margarine, raw cocoa beans, cocoa butter or Lucca's winterized cottonseed oil. / Graduation date: 1987

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Thesis (Master)--İzmir Institute of Technology, İzmir, 2004. / Includes bibliographical references (leaves. 82-84).

Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [129]-140).

Thesis (Ph. D.)--University of Wisconsin, 1966. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.