Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.

Thesis (doctoral)--Lund University, 1997. / Added t.p. with thesis statement inserted.

Diss. (sammanfattning) Umeå : Umeå universitet, 2009. / Härtill 4 uppsatser.

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2006. / Härtill 4 uppsatser.

Thesis--Copenhagen. / Includes bibliographical references.

Submitted by Fabíola Silva (fabiola.silva@famerp.br) on 2018-02-07T18:29:25Z No. of bitstreams: 1 ulissescamargo_tese.pdf: 700757 bytes, checksum: 68231dab9197a8717320c0e887f2b2f6 (MD5) / Made available in DSpace on 2018-02-07T18:29:25Z (GMT). No. of bitstreams: 1 ulissescamargo_tese.pdf: 700757 bytes, checksum: 68231dab9197a8717320c0e887f2b2f6 (MD5) Previous issue date: 2016-09-22 / Introduction. The spondyloarthritis encomprises a group of diseases strongly associated with HLA-B*27 gene. It has been proposed that genes not belonging to the major histocompatibility complex human influence the genesis of these diseases especially in patients HLA-B*27 negative. Objectives. The aim of this study was to test the hypothesis that the antigens of the ABO, Secretor and Lewis histo-blood systems are associated with spondyloarthritis, especially ankylosing spondylitis (AS). Material and methods. Three hundred and ninety-four patients with clinical suspicion of spondyloarthritis sent for identification of HLA-B*27 gene were analyzed. One hundred and nineteen (30.2%) had confirmed the diagnosis of spondyloarthritis according to the ASAS criteria. The remaining 275 (69.8%) were used as controls. The identification of HLA-B*27 gene was performed using the PCR-SSOP method. The identification of the antigens of the ABO, Secretor and Lewis histo-blood systems was performed using hemagglutination and PCR-RFLP methods. The exact Fisher's test, the chi-square, and the values of Odds Ratio (OR) and Confidence Interval set at 95% were calculated using the GraphPad INSTAT software, accepting the error of 5%. Results. No statistically significant differences were observed in the frequency of antigenic profiles of ABO (χ2: 1.152; p = 0.764; GL: 3), Secreto (χ2: 0.779; p = 0.377; GL: 1) and Lewis (χ2: 1.853; p = 0.396; GL: 2) histo-blood groups between patients and controls. The Lea antigen was more frequent in patients with AS compared to controls (OR: 1.833; 95% CI: 1025- 3284, p = 0.053). This antigen was strongly associated with AS in HLA-B*27 negative patients compared to controls (OR: 4.469; 95% CI: 1931-10342; p = 0.0007). This association remained only in males in the absence of HLA-B*27 gene (OR: 6.880; 95% CI: 1852-25564; p = 0.004). Conclusions. AS is associated to the Lea antigen in HLAB* 27 negative male patients. / Introdução. As espondiloartrites compreendem um grupo de doenças fortemente associadas ao gene HLA-B*27. Tem sido proposto que genes não pertencentes ao complexo principal de histocompatibilidade humano influenciam a gênese destas doenças especialmente nos pacientes HLA-B*27 negativos. Objetivos. O objetivo deste estudo foi testar a hipótese de que os antígenos dos sistemas histo-sanguíneos ABO, Secretor e Lewis estão associados à espondiloartrites, especialmente a espondilite anquilosante (EA). Material e método. Foram analisados 394 pacientes com suspeita clínica de espondiloartrites encaminhados para identificação do gene HLA-B*27. Cento e dezenove (30,2%) tiveram o diagnóstico de espondiloartrite confirmado de acordo com os critérios ASAS. Os 275 (69,8%) restantes compuseram o grupo controle. A identificação do gene HLA-B*27 foi realizada com o uso do método PCR-SSOP. A caracterização dos antígenos dos sistemas histo-sanguíneos ABO, Secretor e Lewis foi realizada com o uso dos métodos hemaglutinação e PCR-RFLP. O teste exato de Fisher, o qui-quadrado, os valores de Odds Ratio (OR) e do intervalo de confiança a 95% foram calculados com o uso do software GraphPad Instat, aceitando o erro de 5%. Resultados. Não foram observadas diferenças estatisticamente significantes nas frequências dos perfis antigênicos dos sistemas histo-sanguíneos ABO (χ2: 1.152; p=0,764; GL: 3), Secretor (χ2: 0.779; p=0,377; GL: 1) e Lewis (χ2: 1.853; p=0,396; GL: 2) de pacientes e controles. Foi observada maior frequência do antígeno Lea em pacientes com EA, comparados aos controles (OR: 1.833; IC 95%: 1.025 – 3.284; p=0,053). Este antígeno mostrou-se fortemente associado à EA em pacientes HLA-B*27 negativos comparados aos controles (OR: 4.469; IC 95%: 1.931 – 10.342; p=0,0007). Esta associação se manteve apenas no gênero masculino na ausência do gene HLA-B*27 (OR: 6.880; IC 95%: 1.852 – 25.564; p = 0,004). Conclusões. A EA está associada ao antígeno Lea nos pacientes masculinos HLA-B*27 negativos.

Antibody-mediated rejection is central to ABO incompatible transplantation as well as to xenotransplantation. The xenoantigen alpha-Gal has a highly analogous carbohydrate structure to the human blood group antigens, and both require memory B cell activation for antibody production. We hypothesize that B cells, reactive to the alpha-Gal xenoantigen and B blood group antigen, require the presence of fully activated T cells in order to survive and proliferate in vitro, contrary to the traditional theory that humoral response to carbohydrate antigens is a T cell-independent process. When we compared the capacity of B cells to proliferate, we observed that activated T cells were necessary for B cell proliferation even in the presence of carbohydrate-derived antigens. A relevant question was also to investigate the role of a specific class of T cells: the CD1d-restricted iNKT cells, in the activation of alpha-Gal and B blood group-reactive B cells. The iNKT cells have the specificity of being reactive to glycolipids and are capable of producing both T helper 1 and T helper 2 cytokine responses. We therefore wanted to determine the role of the iNKT cells as mediators of a T helper 2-type response when B cells were exposed to a glycolipid antigen expressing the alpha-Gal epitope or the human B blood group antigen. We observed that, if the interaction between B cells and iNKT cells is blocked, neither B cell proliferation nor antibody production occurs. These results suggest therefore the importance of the iNKT cell category of T helper cells in the response to alpha-Gal and ABO-blood group glycolipids.

Thesis (M.P.H.)--University of Michigan, 1945.

Thesis (M.P.H.)--University of Michigan, 1945.

Resumo: O sistema sangüíneo ABO (sABO) é o mais importante sistema na compatibilidade de grupos sangüíneos. Muitas pesquisas têm mostrado associações deste sistema com várias doenças infecciosas, inclusive a malária. Este estudo avaliou a associação entre os genótipos do sistema histo-sangüíneo ABO e a malária não grave causada pelo Plasmodium falciparum. A genotipagem dos grupos sangüíneos do sistema ABO foi feita de acordo com o protocolo de PCR/ RFLP, em amostras de indivíduos maláricos e não maláricos de áreas da Amazônia brasileira. O genótipo homozigoto ABO*O01O01 foi prevalente tanto nos maláricos quanto nos doadores de sangue. O genótipo ABO*AB representou cerca de 3% da população infectada e 5% da não infectada. Não foram verificadas diferenças estatisticamente significantes na comparação das freqüências alélicas e genotípicas do sABO entre pacientes e grupo controle, mesmo quando foram analisados apenas indivíduos com infecções puras de P. falciparum. A freqüência do sABO na Amazônia brasileira pode estar relacionada com a baixa freqüência de malária grave pelo P. falciparum. Portanto, os genótipos encontrados no sistema ABO dos indivíduos maláricos e não maláricos pode promover relevantes informações, para o entendimento da epidemiologia da malária grave por P. falciparum na Amazônia brasileira. / Abstract: The ABO blood system (sABO) is the most important system on the blood groups compatibility. Several studies have shown its associations with various infectious diseases, including malaria. This study evaluated the association between the ABO histo-blood genotypes and non-severe malaria caused by Plasmodium falciparum. PCR/RFLP protocol had be used for both ABO blood group system genotyping in malaria suffering individuals and blood donors, from malaria areas of the Brazilian Amazon. The homozygous genotype ABO*O01O01 was prevalent in both malaria and the blood donors. The genotype ABO*AB represented about 3% of the infected population and 5% of non-infected. No statistically significant differences were observed in sABO genotypic and allelic frequencies of patients and the control group, even when individuals were analyzed only with pure infection of P. falciparum. The frequency of sABO in the Brazilian Amazon may be related to the low frequency of non-severe malaria P. falciparum. Therefore, the genotypes found in the ABO blood system in malaric and non-malaric individuals can promote relevant information for the understanding of the severe malaria by P. falciparum epidemiology in the Brazilian Amazon. Keywords: Malaria; ABO blood group system; Plasmodium falciparum. / Orientador: Ricardo Luiz Dantas Machado / Coorientador: Luiz Carlos de Mattos / Banca: Carlos Eugênio Cavasini / Banca: Irineu Luiz Maia / Mestre