Root cap cells of two cotton species elicited a specific chemotactic response in zoospores of Pythium dissotocum. When roots of cotton seedlings were placed into a suspension of P. dissotocum zoospores, there was immediate attraction, accumulation and encystment exclusively in the root cap cell region. Furthermore, root cap cells remained attractive when isolated from the root: attraction, accumulation, and encystment on individual root cap cells occurred within seconds after contact. Zoospores penetrated and killed isolated root cap cells within 15-30 minutes, and seedlings died within 24 hours. In contrast, zoospores of P. catenulatum, which exhibited a chemotactic response to roots of Bentgrass, were not attracted to and did not infect seedlings or isolated root cap cells of cotton. Preliminary studies indicate that both Pythium species are capable of infecting cotton seedlings in sand culture, though it is not known if either are pathogens on cotton grown in the field.

No description available.

No description available.

No description available.

This study set out to investigate the effects of level of nitrogen supply on the root dynamics, carbon (C) and nitrogen (N) return to soil from root turnover, decomposition, and stability of improved grasslands. Field studies involved a two-year field experiment, where plant species composition, above ground biomass production, root production, litter decomposition and short term soil organic matter (SOM) dynamics, as a response to varying levels of inorganic fertiliser nitrogen supply were studied. The results showed that root dynamics are strongly affected by N fertilisation, with an increase in root production and death with increasing N supply. Reduced water availability lead to a greater root disappearance rate and the production of roots with a shorter lifespan. Root decomposition was strongly influenced by the age of the grass ley, which also exerted a strong influence on the structure of the soil microbial communities (SMC). Rate of litter decomposition and SMC structure were also influenced by the level of N supply. Level of mineral N supply and age of the grass ley also influenced the formation and stability of water stable aggregates, the microbial community structure and microbial community function within the aggregates. Mineral N applications are likely to influence SOM and soil nutrient dynamics. The finding that climatic conditions, and in particular water availability, had the strongest impact on both above and below ground productivity, and strongly influenced the amount of C, N and root/shoot biomass returned to soils, is of great importance in helping to make more accurate predictions of the response of plant communities to projected changes in the global climate.

Condensed tannins or proanthocyanidins (CTs) are polymeric flavonoids and common plant secondary metabolites. They are defined by their protein binding capacity, and anti-oxidant and metal chelating properties. Known functions of CTs include anti-herbivore and anti-microbial defenses. Chemical ecology studies, especially on CTs, have focussed almost exclusively on foliar chemistry and above-ground ecological interactions. However, CT concentrations in roots can match and far exceed those found in leaves, particularly in woody plant species. This suggests that CTs are also likely to have important ecological and physiological roles below-ground. A review of the literature suggests three potential roles of root CTs: 1) defense against soil pathogens and root herbivores; 2) facilitating adaptation to toxic soils through heavy metal chelation, and; 3) facilitating or modulating nutrient uptake through the binding of nutrient cations. In this thesis the relationship between root CTs and nutrient uptake will be analysed in P. tremula x alba. Localization of CTs was determined through both quantitative and histochemical techniques. Quantitation of CTs with the 1-butanol:HCl assay clearly showed that CT concentration was higher in the white root zone compared to the brown root zone or cork zone. This is inconsistent with the term ‘condensed tannin zone’ that is commonly used to describe the macroscopic brown zone of the root between the white zone and the cork zone in Pinus. The tissue specific localization of CTs in roots was determined using the CT-specific stain, 4-dimethylaminocinnamaldehyde (4-DMACA), on embedded longitudinal and fresh cross sections. These stained sections showed that CTs were present in cells on the root surface, specifically accumulating in the root cap and epidermal cells. CTs were also sporadically present in the cortical cells of the young, white root zones. CT concentration declined as distance from the root tip increased. The pattern of CT distribution, as indicated by intensity of the staining, corresponded directly to the quantitative assay results. Growing poplars under low nitrogen availability stimulated higher root CT concentrations than when they were grown under high nitrogen availability, suggesting a link between nitrogen and CTs. Since CTs are known to bind cations in vivo, I hypothesized that root CTs may modulate or facilitate nutrient uptake by binding positively charged nutrient ions. The Microelectrode Ion Flux Measurement (MIFE) technique was used to analyse specific fluxes of NH4+, NO3- and Ca2+ at specific root locations in poplar roots, and test for spatial correlation with CT localization. This technique was also used to compare the NH4+, NO3- and Ca2+ fluxes in transgenic high-CT and wild-type lines of Populus tremula x alba to test the impact of CTs on nutrient uptake directly. No correlation was found between fluxes of NH4+, NO3- or Ca2+ and CT distribution. The data indicate that root CTs do not influence nutrient uptake in Populus. This study provides data regarding the pattern of CT distribution as well as fundamental information on nutrient uptake in P. tremula x alba roots. It provides new knowledge that will be used to stimulate investigations on other potential roles of root CTs. / Graduate

Master of Science / Agronomy / Dorivar A. Ruiz Diaz Suarez / The effect of tillage on crop yield, early growth, and soil nutrient stratification can be influenced by fertilizer placement. In addition, deeper root systems can enhance the crop ability to uptake water and nutrients. A thorough understanding of how these factors interact can result in increased grain yields and profitability for the producer. Three studies were completed to describe and evaluate different aspects of crop root system and response to fertilizer placement and tillage. The objective of the first study was to characterize the root system of two genotypes of corn (Zea mays) and soybean (Glycine max (L.) Merr.) using image analysis in the greenhouse and in the field, as well as evaluate dry weight accumulation and nutrient uptake patterns by shoot and root plant parts for both crops. Two different genotypes of each crop were sampled during the growing season to access root characteristics such as biomass, length, surface area, average diameter and volume. Significant differences were found in corn where the P1151 AM hybrid had greater root length, surface area and volume than the P1105 AM hybrid. In soybean, the differences were found in nutrient uptake with overall greater nutrient uptake values for the poor drainage variety (PD) compared to the good drainage variety (GD). The objective of the second study was to evaluate the effect of fertilizer placement and tillage system on corn with different genotypes. Three fertilizer treatments were combined with two different corn genotypes selected based on contrasting root systems and two different tillage systems. The three fertilizer placements were sub-surface band, broadcast, and control. The two hybrids of corn used were a P1151 AM hybrid and P1105 AM hybrid. The two tillage systems were no-till (NT) and strip-till (ST). Corn hybrids showed different response in root biomass but did not show a consistent response in other characteristics evaluated. Broadcast and sub-surface band increased nutrient uptake and grain yields over the control but were not significantly different from each other. Tillage showed no difference in corn response. The objective of the third study was to evaluate the effect of fertilizer placement and tillage system on contrasting soybean genotypes. Three fertilizer treatments were combined with two different genotypes selected based on contrasting root systems and two different tillage operations. The three fertilizer placements were sub-surface band, broadcast, and control. The two varieties of soybean used were one recommended for poor drainage (PD) and one recommended for good drainage (GD). The two tillage operations were NT and ST. Soybean root biomass differences were observed by varieties. Sub-surface band treatment favored early soybean growth, biomass and P uptake at the V3 growth stage, but it did not turn into yield increase. Soybean grain yields did not respond to fertilization in this study. Yield was affected significantly by variety selection and response varies by site-year.

A research report submitted to the Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, in partial fulfilment of the requirements for the degree of Master of Science Johannesburg, 2012 / This experiment was designed to evaluate whether an allogeneic fibrin-fibronectin protein concentrate (AFFP) can not only prevent ankylosis and root resorption of autotransplanted roots during healing but contribute to regenerate a periodontal attachment as well. In two adult male baboons (Papio ursinus), four horizontal alveoli, 2 to 3 mm deep, were prepared bilaterally in the buccal alveolar and basal bone adjacent to the first and second mandibular molars to receive the roots of the adjacent two molars. Following hemisection, the first and second mandibular molars were extracted, the coronal two-thirds of the roots were planed to remove the remnants of the periodontal ligament and cementum and a notch was placed at the junction between the planed and non-planed surfaces. The planed surfaces were demineralised with citric acid at pH 1 for 3 min. Before transplantation, the crowns were resected and the experimental roots and alveoli were coated with the AFFP prepared from pooled fresh-frozen baboon plasma. The animals were killed 55 days after the transplantations. Histometrical evaluation was performed on serial sections cut in a bucco-lingual direction parallel to the long axis of the transplanted roots. An analysis of variance, in relation to the extent of ankylosis and root resorption, revealed minimal differences between the treatments of experimental and control roots both in the planed and non-planed sections. In this primate autotransplantation model, the treatment with AFFP did not prevent ankylosis and root resorption and did not result in the establishment of a new periodontal attachment.

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