Diss. Univ. Hamburg, 2007. / Quellenverzeichnis: S. 343-362.

S. coelicolor uses an active chromosome partitioning system for developmentally-regulated genome segregation, which is associated with spore formation. There are four known trans-acting segregation proteins (ParA, ParB, ParJ and Scy) and cis-acting centromere-like sites (parS). parA encodes a Walker-type ATPase that is required for efficient DNA segregation and proper placement of the ParB-parS nucleoprotein complexes. A paralogue of ParA is encoded by the S. coelicolor genome, SCO1772 (named ParH), that has 45% identical residues to ParA. In S. coelicolor aerial hyphae, a ∆parH mutant produces 5% of anucleate spores. In this study, ParH was identified as a novel interaction partner of S. coelicolor ParB. However, a Walker A motif K99E substitution in ParH and removal an N-terminal extension in ParH impaired interaction between ParH and ParB, as judged by bacterial two-hybrid analyses. ParH-EGFP localization resembles the evenly-spaced localization pattern of ParH-EGFP in aerial hyphae, which might suggest that ParH colocalizes with ParB. A parH-null mutant appears to be unable to properly organize the oriC regions within a subset of prespores, as judged by ParB-EGFP foci. In this study, through a random chromosomal library screening, a novel protein that interacts with ParA and ParH was also identified. HaaA (ParH and ParA Associated protein A) is required for proper chromosome segregation and is one of the 24 signature proteins of the Actinomycetes that are not found in other bacterial lineages. A bacterial two-hybrid analysis showed that HaaA interacts with itself and interaction between ParH and ParA was through the C-terminal unstructured region. Interaction between HaaA and ParA and ParA-like proteins was conserved in other Actinomycetes, such as S. venezuelae, C. glutamicum and M. smegmatis. There was no evidence for interaction with other tested segregation proteins. In addition, a haaA insertion-deletion mutant strain revealed that loss of HaaA affected chromosome segregation (6% anucleate spores) and HaaA-EGFP localizes within spores of the mature spore chains. Together these data revealed new information to further understand chromosome segregation in S. coelicolor. / Bayer School of Natural and Environmental Sciences; / Biological Sciences / PhD; / Dissertation;

There is no abstract available for this dissertation.

Diss. Umeå : Univ., 2004.

Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 5 uppsatser.

Thesis (Ed. D.)--Illinois State University, 1974. / Title from title page screen, viewed Oct. 26, 2004. Dissertation Committee: Ronald Laymon (chair), J.H. McGrath, Larry Kennedy, Charles Sherman, Vern Pohlman. Includes bibliographical references (leaves 137-139) and abstract. Also available in print.

Thesis--University of Wisconsin. / Microfilm-xerography. Ann Arbor, Mich. : University Microfilms, 1972.--21 cm. Description based on print version record. Includes bibliographical references (leaves 404-411).

Thesis--University of Wisconsin--Madison. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 247-256).

Thesis (M.S.)--University of Wisconsin--Madison, 1974. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Syftet med denna studie är att undersöka hur förvandlingen kring synen påmiljonprogramsområdena gått till. Miljonprogrammet som till en början sågs som en modern satsning som skulle lösa de akuta problemen på den svenska bostadsmarknaden men som under årens gång övergått till något negativt där det talas om stigmatiserade utanförskaps områden som domineras av sociala problem. Kritiken mot områdena visar att boende i dessa områden ofta beskylls för de problemen som finns, kopplat till etniska och sociala faktorer. Studien undersöker huruvida den kritik som lyfts kring miljonprogrammet, dess områden och boende stämmer överens med verkligheten. Med hjälp av olika teorier som, stigmatisering, gentrifiering och begreppet segregation undersöka de bakomliggande faktorerna till att boende i miljonprogramsområdena beskylls för de problem som finns samt för att undersöka om denna bild stämmer överens med verkligheten. / The purpose of this qualitative study is to investigate how the view of the million programareas changed from positive to negative. The million program, which at first was seen as amodern investment that would solve the problems that existed in the Swedish housing market, but through the years turned into something negative where areas are spoken of as problematic. Criticism of the areas shows that residents in these areas are often blamed for the problems that exist, linked to ethnic and social factors. The purpose of this studiy is to examine whether the criticism raised about the millionprogram and its areas is in line with reality. Using, Ervin Goffmans theory of stigmatization, Ruth Glasss definition of gentrification and the notion segregation, examine the underlying factors of people living in the million programareas that are often blamed for the problems that exist and to examine if this is a true and fair picture or not.