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A modification of the method of Hewish and Burgoyne for the isolation of rat liver nucleiWoll, Wesley George January 1980 (has links)
No description available.
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The development of micro capillary film membranes for separation processesBonyadi, Sina January 2012 (has links)
No description available.
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An improved isolation method for achilleine, an alkaloid from Achillea millefolium linneLanders, Roy March, 1934- January 1958 (has links)
No description available.
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Biocatalysis of immobilized lipoxygenase in organic solvent mediaDioum, Ndeye. January 2000 (has links)
The biocatalysis of purified soybean lipoxygenase (LOX) (EC 1.13.11.12) using linoleic acid as a substrate model was investigated in selected organic solvent media, including chloroform, dichloromethane, hexane, iso-octane, octane and toluene. The results indicated that there was a 2.6 fold increase in LOX activity in the monophasic iso-octane medium compared to that obtained in the aqueous medium. In addition, the optimum concentration of octane and iso-octane in the biphasic medium containing the organic solvent and Tris-HCl buffer solution, was determined to be 3.5 and 4%, respectively, for LOX activity which resulted in a further increase in LOX activity. The immobilized LOX showed better substrate specificity towards linoleic acid, followed by arachidonic acid. The enzymatically-catalyzed end-products were investigated, and the results indicated that different proportions of the 9- and 13-HPOD isomers were produced by LOX biocatalysis depending on the reaction medium used and the free or immobilized state of the enzyme. (Abstract shortened by UMI.)
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Production and characterization of bioactive peptides from soy fermented foods and their hydrolysatesGibbs, Bernard F. January 1999 (has links)
Biologically active peptides are found in the amino acid sequence of bacterial, fungal, plant and animal proteins. They are among the most potent pharmacologically active agents. Examples are venom toxin (mellitin), hormones (oxytoxin), opioids (beta-endorphin) and enzyme inhibitors (hirudin). These bioactive peptides are released from proteins by enzymatic proteolysis by processes such as gastrointestinal digestion or food processing. / Soybeans were fermented with Bacillus subtilis ATCC 41332 and Rhizopus oligosporus NRRL 2710 to produce tempeh and natto, respectively. Samples were taken throughout the fermentation and analysed for biochemical changes. Protease activity and ammonia production detected in the early stage of the tempeh preparation suggested that protein was used as a carbon source during that period and contributed to a rise in pH. Bacillus subtilis did not produce ammonia and maintained a constant pH throughout the fermentation. The total peptides produced were at a maximum at the end of the fermentation cycles. Angiotensin-converting enzyme inhibitory activity increased throughout both fermentations. A method was developed to monitor the production of biogenic amines throughout the fermentation. The levels of biogenic amines increased dramatically as the fermentation proceeded until maturity was achieved. In the tempeh fermentation, the polyamines levels rose from the initial 19 ppm to final concentration of 862 ppm, with the largest increase in histamine (616 ppm) followed by putrescine (204 ppm). These compounds also contributed to the pH rise from 3.8 to 6.8 in 24 h. In the Bacillus fermentation, the total polyamines at the end of the fermentation was 110 ppm with the largest increase in putrescine, followed by cadaverine. / Soy hydrolysate and the soy fermented foods, natto and tempeh, were deglycosylated and treated with proteolytic enzymes (plasma proteases, kidney homogenate, pronase, pepsin, thermolysin, trypsin, chymotrypsin and proteinase K) to produce oligopeptides. Several peptides were isolated, purified and characterized. The peptides had a range of biological activities---angiotensin converting enzyme inhibitory, antithrombotic, surface tension, antibacterial, anti-oxidant and insulin-modulating activities. Three potent ACE inhibitors, three thrombin inhibitors, five peptides with surface-active properties and one peptide with antibacterial activity were identified. They were all derived from glycinin and were found in the plasma protease digest, the kidney homogenate digest and the pronase digest of fermented foods. Another sequence ELLVYLL possessed good surface active properties but its precursor could not be identified. However, it was analogous to a peptide produced by Bacillus subtilis , and was probably synthesized during fermentation. Peptide analogs were synthesized and evaluated. They showed similar activities. Other sequences of known inhibitors, TPKDFEEIPEE, FPRGGG and DFEEIPEEL, were found to be competitive substrates for ACE.
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A static model of high gradient magnetic separation /Nesset, Jan Edward. January 1980 (has links)
No description available.
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An immunochemical analysis of goldfish brain proteins using antisera raised against memberane fractions /Manseau, Maryse January 1989 (has links)
No description available.
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Sparger study in flotation columnsXu, Manqiu January 1987 (has links)
No description available.
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Chemical separation in electrostatic precipitatorsSalcedo, R.L.R. (Romualdo L.R.) January 1981 (has links)
This work was carried out to investigate the mechanisms of solid-solid separation in electrostatic precipitators. The separation of mixture of pure compounds from each other and the components of an industrial dust has been studied in a wire-and-duct type laboratory-scale electrostatic precipitator. Particle size distribution affected the separation most, but both the dielectric constant and electrical resistivity of the dust can be important. Operating voltage and air velocity in the precipitator also affected the separation along the precipitator length. The SRI computer model of precipitator performance gave reasonable predictions of separation under conditions of negligible re-entrainment. / An apparently unique relationship between separation and recovery was experimentally observed with components which do not re-entrain appreciably; a similar behaviour is theoretically predicted from the Deutsch Equation of collection efficiency or the SRI model.
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Polishing H₂S from coal gasification streams using a high temperature electrochemical membrane separation processRobinson, Jeffrey Scott 12 1900 (has links)
No description available.
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