眼動儀目前的主要用途在分析使用者的觀看行為，藉以改善介面的設計，或幫助身體有缺陷但眼睛還能轉動的使用者與外界溝通。隨著相關技術的發展，眼動儀將可能如同滑鼠、鍵盤一般，成為使用者輸入裝置的選項。本論文的目的在於設計並實作低成本之穿戴式與遠距眼動儀，並將其應用於以視線軌跡為基礎的人機介面，希望能夠增進人與電腦之間的互動方式。由於眼動儀會受到雜訊與角膜反射點等的影響，本研究提出利用瞳孔周圍暗色點比例較高的特性，增加定位之準確性，以改善眼動儀之精確度，此外，頭部的移動亦會造成眼動儀在計算投射位置時之誤差，本研究也針對這個問題提出因應之解決方案。利用前述製作的眼動儀，本論文實作數個以視線軌跡為基礎的人機介面，包括視線軌跡網頁瀏覽器、強化眼睛注視照片區域、井字遊戲、互動式媒體等，並利用眼動儀記錄使用者觀看手機介面的行為。 / Eye tracker, a device for measuring eye position and movements, has traditionally been used for research in human visual system, psychology and interface design. It has also served as an input device for people with disability. With recent progresses in hardware and imaging technology, it has the potential to complement, even replace popular devices such as mouse or keyboard for average users to communicate with the computer. It is the objective of this research to design and implement low-cost head-mounted and remote eye trackers and subsequently develop applications that take advantage of gaze-based interactions. Specifically, we improve the precision of the tracking result by designing a new pupil detection algorithm as well as compensating for head movement. We then present several gaze-based user interfaces, including eye-controlled web browser, attention-based photo browser, interactive game (tic-tac-toe) and media design. We also investigate the feasibility of utilizing the eye trackers to analyze and evaluate the design of mobile user interface.