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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

牛樟芝複方飲品抗疲勞功能之評估 / Evaluation of Anti-fatigue Properties of Antrodia Camphorata Drink

劉仁溥, Ren-Pu Liu January 1900 (has links)
牛樟芝 (Antrodia camphorata; AC) 為台灣特有的藥用真菌,是常見的傳統 中藥,富含三萜類化合物及多醣體。本研究使用牛樟芝、葛花、枳椇子與山楂共 四種萃取物製成之牛樟芝複方飲品 (A. camphorate drink, ACD)為原料,評估此一 複方飲品之抗疲勞功效。本研究從運動表現、疲勞生化指標、肌肉損傷生化指數 以及能量儲存等做為抗疲勞評估標準。將 5 週齡雄性 ICR (Institute of Cancer Research) 小鼠,分為三組,每組各 10 隻,分組如下:(1) 對照組 (vehicle, V); (2) 餵食 15.625 mL/kg 牛樟芝複方飲品 (ACD-1X);(3) 餵食 31.25 mL/kg 牛樟芝 複方飲品 (ACD-2X),連續經口餵食 28 天後進行以下試驗: 1. 測試小鼠前肢抓 力、2. 測試 5%負重游泳力竭時間、3. 進行游泳運動 15 分鐘後立即進行採血觀 察乳酸、血氨、血糖及肌酸激酶、4. 小鼠犧牲後,採集肝臟及肌肉組織進行肝醣 分析與切片。結果顯示,ACD-1X 組 (1124 g) 與 ACD-2X 組 (125 6 g) 在前肢 抓力與對照組 (95 6 g) 相比,分別顯著提升 1.19 與 1.32 倍 (p < 0.05)。ACD-1X 組 (56.714.3 min) 與 ACD-2X 組 (60.717.7 min) 在游泳力竭時間與對照組 (18.23.7 min) 比較分別延長 3.12 及 3.34 倍,均有顯著差異 (p < 0.05)。游泳運 動結束後,ACD-1X 組與 ACD-2X 組之乳酸、血氨及肌酸肌酶均顯著低於對照組 (p < 0.05),而血糖方面則顯著高於對照組(p < 0.05)。肌肉肝醣部分,ACD-1X 組 (1.79 ± 0.12 mg/g) 與 ACD-2X 組 (2.36 ± 0.21 mg/g) 相較於對照組 (1.54 ± 0.06 mg/g),2倍牛樟芝複方飲品組顯著提升1.53倍。肝臟肝醣部分,ACD-1X組 (48.9± 4.1 mg/g) 與 ACD-2X 組 (54.1 ± 5.8 mg/g) 與對照組 (27.0 ± 2.4 mg/g) 比較分別 增加 1.18 及 2.00 倍。研究結果顯示,牛樟芝複方飲品具有增加肝臟及肌肉組織 中的肝醣儲存量,且在游泳運動過程中降低血乳酸、血氨及肌酸激酶的產生。進 一步延長負重游泳力竭的時間及前肢抓力表現。因此,本研究證實牛樟芝複方飲 品具有抗疲勞及提升運動表現之功效。 / Antrodia camphorata is an endemic medical mushroom in Taiwan and has been reported to have multi-biological activities such as anti-fatigue, liver protection, anti-cancer, and anti-inflammation. This study was designed to ascertain the ergogenic and anti-fatigue properties of an A. camphorata drink (ACD) (comprising A. camphorata, Pueraria flos flowers, Hovenia dulcis Thunb, and Crataegus pinnatifida) by forelimb grip strength, load-weighted swimming test, and biochemical examinations in mouse model. Thirty male ICR mice (5-week-old) were randomly divided into three groups (n=10 per group): (1) vehicle; (2) a recommended dosage of 15 mg per 75 mL once per day for adult human (ACD-1X); (3) a 2-fold of recommended usage for adult human (ACD-2X). Mice were given orally either vehicle or A. camphorate drink by gavage for 28 days. The groups ACD-1X and ACD-2X showed significant increases in forelimb grip strength, swimming time to exhaustion skeletal, and muscle glycogen as compared to the vehicle group. Blood lactate and ammonia levels in the ACD-1X and ACD-2X groups were significantly lower than in the vehicle group post a swimming test. Moreover, the activity of plasma creatine kinase (CK), a marker of muscular damage, was significantly decreased in the ACD-1X and ACD-2X groups than vehicle group after swimming exercise. These results suggest that A. camphoratahas great potential for application in relevant fields for its ergogenic and anti-fatigue activities. / 第一章 緒論 .........................................................................................................................1 第一節 研究目的..............................................................................................................2 第二章 文獻探討 .................................................................................................................3 第一節 牛樟芝成份及相關研究......................................................................................3 第二節 葛花成份及相關研究..........................................................................................6 第三節 枳椇子成份及相關研究......................................................................................7 第四節 山楂成份及相關研究..........................................................................................8 第五節 運動與疲勞..........................................................................................................9 第三章 材料與方法 ...........................................................................................................12 第一節 實驗測試樣品....................................................................................................12 第二節 實驗動物之飼養與實驗流程............................................................................14 第三節 血液與組織樣本之收集與前處理....................................................................16 第四節 統計分析............................................................................................................17 第四章 結果與討論 ...........................................................................................................18 第一節 補充牛樟芝複方飲品對於小鼠游泳運動能力表現之測試............................18 第二節 補充牛樟芝複方飲品對於小鼠游泳運動後血液中乳酸、血氨、血糖濃 度以及 CK 活性之分析..................................................................................................19 第三節 補充牛樟芝複方飲品對於小鼠前肢抓力表現之測試....................................20 第四節 補充牛樟芝複方飲品對於小鼠肌肉與肝臟部位肝醣含量之分析................21 第五章 結論 .......................................................................................................................22 參考文獻.............................................................................................................31

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