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Canine Pure Platelet-Rich Plasma for Regenerative Medicine and Platelet Research: Protocol OptimizationMonobe, Marina Mitie 09 December 2016 (has links)
Platelet-rich plasma (PRP) can be widely used in veterinary medicine in different areas. Studies using PRP frequently use different methodologies making for difficult comparison. The objective of this study was to evaluate the purity and platelet activation of a PRP protocol. A total of 18 blood samples were drawn from six dogs, collected once per week over a total of three weeks. Blood samples were centrifuged six times at 300g for 5 min. Ultra-pure PRP (OP) was obtained by adding PRP a Optiprep 1.063g/mL density barrier and centrifuged at 350g for 15 min. Mean platelet recovery from whole blood was 62.90% in PRP and 45.24% in OP. PRP and OP showed high platelet purity; blood cell contamination <0.01%. Flow cytometry for platelet activation markers was consistent with minimal platelet activation. This study describes the optimization of PRP protocol with high platelet purity, minimal platelet activation, high reliability and reproducibility.
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Cushioned centrifugation of stallion semen: factors impacting equine sperm recovery rate and qualityWaite, Jessica Arlene 10 October 2008 (has links)
Centrifugation of stallion semen is an integral part of the cryopreservation procedure, primarily allowing for the concentration of sperm and removal of seminal plasma. In addition, centrifugation is required for maximizing spermatozoal quality in semen from some stallions subjected to cooled transport, because of the detrimental effects of long-term exposure to high levels of seminal plasma. The centrifugation process, however, has potential deleterious effects, including reduction in sperm quality as well as loss of sperm numbers. Since centrifugation plays such a crucial role in semen processing, two experiments were designed to evaluate more efficient centrifugation methods to meet the demands of the equine industry. In Experiment 1, semen was centrifuged in two different tube types (nipple- or conical-bottom), using a cushioned technique (Eqcellsire® Component B) with two different extenders (opaque-INRA96 or clear-HGLL). For Experiment 2, nipple-tube centrifugation was conducted at two different g forces (400 or 600) for 20 min, using three different iodixanol cushion media, Eqcellsire® Component B, OptiPrep[TM], or Cushion Fluid[TM]. Regardless of tube or extender types, centrifugation of semen resulted in sperm recovery rates ≥90%; however, centrifugation in INRA 96 extender yielded higher sperm motility values than did centrifugation in HGLL extender (P < 0.05). Cushion type or g force did not impact post-centrifugation semen quality, based on the laboratory values measured (P > 0.05). These results indicate that cushioned centrifugation of stallion semen in either conical-bottom or nipple-bottom tubes can yield a high sperm harvest, while maintaining sperm function. An optically opaque extender, as is typically used in the equine breeding industry, can be used to achieve this goal. The fertility rate (94%; 131/140) following cushioned semen centrifugation in a commercial program this past year indicates that these laboratory results are transferable to the clinical setting.
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Cushioned centrifugation of stallion semen: factors impacting equine sperm recovery rate and qualityWaite, Jessica Arlene 15 May 2009 (has links)
Centrifugation of stallion semen is an integral part of the cryopreservation procedure, primarily allowing for the concentration of sperm and removal of seminal plasma. In addition, centrifugation is required for maximizing spermatozoal quality in semen from some stallions subjected to cooled transport, because of the detrimental effects of long-term exposure to high levels of seminal plasma. The centrifugation process, however, has potential deleterious effects, including reduction in sperm quality as well as loss of sperm numbers. Since centrifugation plays such a crucial role in semen processing, two experiments were designed to evaluate more efficient centrifugation methods to meet the demands of the equine industry. In Experiment 1, semen was centrifuged in two different tube types (nipple- or conical-bottom), using a cushioned technique (Eqcellsire® Component B) with two different extenders (opaque-INRA96 or clear-HGLL). For Experiment 2, nipple-tube centrifugation was conducted at two different g forces (400 or 600) for 20 min, using three different iodixanol cushion media, Eqcellsire® Component B, OptiPrep™, or Cushion Fluid™. Regardless of tube or extender types, centrifugation of semen resulted in sperm recovery rates ≥ 90%; however, centrifugation in INRA 96 extender yielded higher sperm motility values than did centrifugation in HGLL extender (P < 0.05). Cushion type or g force did not impact post-centrifugation semen quality, based on the laboratory values measured (P > 0.05). These results indicate that cushioned centrifugation of stallion semen in either conical-bottom or nipple-bottom tubes can yield a high sperm harvest, while maintaining sperm function. An optically opaque extender, as is typically used in the equine breeding industry, can be used to achieve this goal. The fertility rate (94%; 131/140) following cushioned semen centrifugation in a commercial program this past year indicates that these laboratory results are transferable to the clinical setting.
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Cushioned centrifugation of stallion semen: factors impacting equine sperm recovery rate and qualityWaite, Jessica Arlene 15 May 2009 (has links)
Centrifugation of stallion semen is an integral part of the cryopreservation procedure, primarily allowing for the concentration of sperm and removal of seminal plasma. In addition, centrifugation is required for maximizing spermatozoal quality in semen from some stallions subjected to cooled transport, because of the detrimental effects of long-term exposure to high levels of seminal plasma. The centrifugation process, however, has potential deleterious effects, including reduction in sperm quality as well as loss of sperm numbers. Since centrifugation plays such a crucial role in semen processing, two experiments were designed to evaluate more efficient centrifugation methods to meet the demands of the equine industry. In Experiment 1, semen was centrifuged in two different tube types (nipple- or conical-bottom), using a cushioned technique (Eqcellsire® Component B) with two different extenders (opaque-INRA96 or clear-HGLL). For Experiment 2, nipple-tube centrifugation was conducted at two different g forces (400 or 600) for 20 min, using three different iodixanol cushion media, Eqcellsire® Component B, OptiPrep™, or Cushion Fluid™. Regardless of tube or extender types, centrifugation of semen resulted in sperm recovery rates ≥ 90%; however, centrifugation in INRA 96 extender yielded higher sperm motility values than did centrifugation in HGLL extender (P < 0.05). Cushion type or g force did not impact post-centrifugation semen quality, based on the laboratory values measured (P > 0.05). These results indicate that cushioned centrifugation of stallion semen in either conical-bottom or nipple-bottom tubes can yield a high sperm harvest, while maintaining sperm function. An optically opaque extender, as is typically used in the equine breeding industry, can be used to achieve this goal. The fertility rate (94%; 131/140) following cushioned semen centrifugation in a commercial program this past year indicates that these laboratory results are transferable to the clinical setting.
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Effect of Density Gradient Centrifugation on Quality and Recovery Rate of Equine SpermEdmond, Ann J. 2009 May 1900 (has links)
Density gradient centrifugation of sperm is a common assisted-reproduction
procedure in humans used to improve semen quality. The technique allows sperm
separation based on their isopycnic points. Sperm with morphologic abnormalities are
often more buoyant, leading to their retention above centrifuged density gradients, with
structurally normal sperm passing through the gradient. Three experiments were
conducted to evaluate the effects of tube size, sperm number following centrifugation,
and density gradient volume (height) on stallion sperm quality and recovery rate in
sperm pellets following centrifugation. In all three experiments, equine semen was
initially centrifuged to increase sperm concentration. In Experiment 1, one-mL aliquots
were layered over EquiPure? Bottom Layer (1-Layer) or over-tiered EquiPure? Top
and Bottom Layers (2-Layer). For Experiment 2, one-mL aliquots were layered over
three different heights of EquiPure? Bottom Layer in 15-mL or 50-mL conical-bottom
tubes. For Experiment 3, four different aliquots containing a sperm load of 1-4x were
layered over a constant volume of EquiPure? Bottom Layer in 15-mL or 50-mL conical bottom tubes. The tubes were then centrifuged. Resulting sperm pellets were evaluated
for morphologic quality, DNA integrity, motility and recovery rate.
Sperm-EquiPure? centrifugation yielded improvements in motility, morphology
and DNA integrity parameters (P<0.05), as compared to controls. The 1-Layer method
resulted in a higher recovery rate than the 2-Layer method (P<0.05). Sperm processed in
the 15-mL tubes yielded higher velocity and higher recovery rates than sperm processed
in the 50-mL tubes (P<0.05). Within tube type, gradient volume did not impact
parameters of semen quality or recovery rate. An increase in sperm number for density
gradient centrifugation resulted in a decreased recovery rate (P<0.05) when 15-mL tubes
were used.
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Cushioned centrifugation of stallion semen: factors impacting equine sperm recovery rate and qualityWaite, Jessica Arlene 10 October 2008 (has links)
Centrifugation of stallion semen is an integral part of the cryopreservation procedure, primarily allowing for the concentration of sperm and removal of seminal plasma. In addition, centrifugation is required for maximizing spermatozoal quality in semen from some stallions subjected to cooled transport, because of the detrimental effects of long-term exposure to high levels of seminal plasma. The centrifugation process, however, has potential deleterious effects, including reduction in sperm quality as well as loss of sperm numbers. Since centrifugation plays such a crucial role in semen processing, two experiments were designed to evaluate more efficient centrifugation methods to meet the demands of the equine industry. In Experiment 1, semen was centrifuged in two different tube types (nipple- or conical-bottom), using a cushioned technique (Eqcellsire® Component B) with two different extenders (opaque-INRA96 or clear-HGLL). For Experiment 2, nipple-tube centrifugation was conducted at two different g forces (400 or 600) for 20 min, using three different iodixanol cushion media, Eqcellsire® Component B, OptiPrep[TM], or Cushion Fluid[TM]. Regardless of tube or extender types, centrifugation of semen resulted in sperm recovery rates ≥90%; however, centrifugation in INRA 96 extender yielded higher sperm motility values than did centrifugation in HGLL extender (P < 0.05). Cushion type or g force did not impact post-centrifugation semen quality, based on the laboratory values measured (P > 0.05). These results indicate that cushioned centrifugation of stallion semen in either conical-bottom or nipple-bottom tubes can yield a high sperm harvest, while maintaining sperm function. An optically opaque extender, as is typically used in the equine breeding industry, can be used to achieve this goal. The fertility rate (94%; 131/140) following cushioned semen centrifugation in a commercial program this past year indicates that these laboratory results are transferable to the clinical setting.
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Measurement of free radicals and their effects on human spermatozoa /Lampiao, Fanuel. January 2006 (has links)
Thesis (MSc)--University of Stellenbosch, 2006. / Bibliography. Also available via the Internet.
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Three-dimensional centrifuge and numerical studies of multiple tunnel interaction /Chung, King Hei. January 2004 (has links)
Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2004. / Includes bibliographical references (leaves 208-217). Also available in electronic version. Access restricted to campus users.
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Fate, survival and growth of fecal coliforms through centrifuge dewatering /Gardner, Julie, January 1900 (has links)
Thesis (M.App.Sc.) - Carleton University, 2007. / Includes bibliographical references (p. 141-157). Also available in electronic format on the Internet.
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Intermittent application of hypergravity by centrifugation attenuates disruption of rat gait induced by 2 weeks of simulated microgravity / 微小重力環境によって惹起されたラットの歩行動作変化は遠心重力による間欠的高重力刺激によって抑制され得るTajino, Junichi 24 September 2015 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(人間健康科学) / 甲第19278号 / 人健博第30号 / 新制||人健||3(附属図書館) / 32280 / 京都大学大学院医学研究科人間健康科学系専攻 / (主査)教授 市橋 則明, 教授 三谷 章, 教授 松田 秀一 / 学位規則第4条第1項該当 / Doctor of Human Health Sciences / Kyoto University / DFAM
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