11 |
Removal of estrone from water with adsorption and UV PhotolysisWen, Huajing 04 May 2006 (has links)
This work investigated the combined technology of adsorption on hydrophobic molecular sieves (zeolites) and direct UV (254 nm) photolysis for removing estrone (E1) from water. The target compound estrone belongs to the group of endocrine-disruptor compounds (EDCs) that are raising more and more concern due to increasing evidence of their adverse estrogenic effects on aquatic organisms and humans. Current wastewater treatment processes remove less than 80% of estrone on average. However, because of its strong biological potency, small amounts are still able to exert adverse estrogenic effects on aquatic systems. Consequently, advanced treatment technologies have been investigated in the hope of reaching higher removal efficiency. Adsorption of estrogens on hydrophobic zeolites in this work is a potential new alternative. Based on the hydrophobic nature of estrogens including E1, two types of zeolites, dealuminated Y (DAY) and silicalite-1, and a type of granular activated carbon Centaur® activated carbon (GAC) were evaluated for adsorption capacity. The results demonstrated that DAY is the best adsorbent for E1 in that 99% E1 can be removed by DAY. Silicalite-1 was the least effective. Moreover, adsorption of E1 to DAY is much faster. Estrone reached adsorption equilibrium in 4 hours on DAY versus 8 days for GAC. The adsorption data of DAY for E1 were fit to the Freundlich and Langmuir equations and the maximum adsorption capacity is estimated as 74 mg E1/g DAY. Direct UV photolysis of E1 in solution was also evaluated. Short-wave UV (ë = 254 nm) degraded E1 in solution much more effectively than long-wave UV-light (ë = 365 nm). No significant increase in degradation of E1 in UV photolysis was found with the addition of hydrogen peroxide. The regeneration of E1-contaminated DAY was investigated by a series of adsorption/direct UV (ë = 254nm) irradiation cycles. No significant deterioration of adsorption capacity of DAY was observed over nine adsorption/regeneration cycles.
|
12 |
The effects of altered prolactin levels on follicular dynamics and endocrine profiles in transitional pony mares /Bennett-Wimbush, Karen, January 1996 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 1996. / Typescript. Vita. Includes bibliographical references (leaves 87-111). Also available on the Internet.
|
13 |
The effects of altered prolactin levels on follicular dynamics and endocrine profiles in transitional pony maresBennett-Wimbush, Karen, January 1996 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 1996. / Typescript. Vita. Includes bibliographical references (leaves 87-111). Also available on the Internet.
|
14 |
Human Steroid Sulfatase: Inhibitor Studies and Photoaffinity LabelingPhan, Chau-Minh January 2010 (has links)
Steroid sulfatase (STS) is considered to be one of the key enzymes contributing to the development of breast cancer. It catalyzes the hydrolysis of inactive sulfated steroids such as estrone sulfate (ES) to inorganic sulfate active steroids such as estrone (E1), a precursor to estradiol (E2), a key stimulator for breast cancer development. Inhibitors of STS are currently being pursued in both academia and industry as potential drugs for treating breast cancer.
A series of 4-substituted estrone and estradiol derivatives were examined as inhibitors of STS. Inhibition of STS with 4-FE1, an irreversible inhibitor of STS previously studied in the Taylor group, can be enhanced by introducing a hydrophobic benzyl group at the 17-positon of 4-FE1. As with 4-FE1, the inhibition was concentration and time-dependent. Only 14% of the activity could be recovered after extensive dialysis. Introducing substituents at the 2-position of 4-formyl estrogen derivatives resulted in loss of concentration and time-dependent inhibition and a considerable decrease in inhibitor affinity. Studies with estrogen derivatives substituted at the 4-position with groups other than a formyl revealed that a relatively good reversible inhibitor can be obtained simply by introducing an electron withdrawing group at this position. These types of inhibitors are non-competitive inhibitors suggesting an alternative steroid binding site.
A series of estrone derivatives were examined as photoaffinity labels of STS. 4-azidoestrone suflate and 4-azidoestrone phosphate exhibited properties that are suitable for photoaffinity labeling studies with STS. These labels may be useful for ascertaining pathways of substrate entry into the STS active site. 16-diazoestrone phosphate was not a photoaffinity label of STS. 2- and 4-azido estrone and 16-diazoestrone all acted as photoaffinity labels of STS. These compounds may be useful for ascertaining pathways of product release from the STS active site.
|
15 |
Analysis of estrone sulphate, testosterone, and cortisol concentrations around time of ejaculation and potential correlation to sexual behavior and sperm characteristics in stallionsSeale, Jennifer 2009 May 1900 (has links)
In the stallion, inconsistent sexual behavior and variable semen quality are
common. This reproductive variability has been attributed to differences in circulating
hormone concentrations. In order to further examine this relationship, 7 miniature
stallions were observed for sexual behavior and semen characteristics. Blood was also
drawn from each stallion 15 min before mating (time -15), immediately following
ejaculation (time 0) and at times following ejaculation (times +15, +30, and +60).
Plasma was later analyzed for concentrations of testosterone (T), estrone sulphate (ES)
and cortisol. Semen was evaluated for volume, sperm concentration and progressive
motility. Sexual behavior was quantified by assigning a libido score to each stallion,
recording reaction time and the number of jumps required for ejaculation.
Upon statistical analysis, data revealed both ES and cortisol increased at the time
of semen collection (P < 0.05), while T did not. Regression analysis revealed that ES
and the ratio of ES to T at times -15, +30, and +60 were negatively correlated to libido scores. Additionally, a positive relationship was found between ES at times -15 and +60
and reaction time, as well as between cortisol at times -15, 0, and +15 and libido scores.
No relationship was observed between T and sexual behavior. However, T at time -15
was positively correlated to progressive motility, and the ratio of ES/T at time -15 was
negatively correlated to progressive motility. No other association was detected between
ejaculate parameters and hormone concentrations. These results not only serve to
enhance understanding of stallion hormone profiles, but also provide further insight into
the hormonal control of sexual behavior and sperm production. This knowledge can be
used to generate improved management techniques for stallions that are inconsistent in
sexual behavior and sperm output.
|
16 |
Human Steroid Sulfatase: Inhibitor Studies and Photoaffinity LabelingPhan, Chau-Minh January 2010 (has links)
Steroid sulfatase (STS) is considered to be one of the key enzymes contributing to the development of breast cancer. It catalyzes the hydrolysis of inactive sulfated steroids such as estrone sulfate (ES) to inorganic sulfate active steroids such as estrone (E1), a precursor to estradiol (E2), a key stimulator for breast cancer development. Inhibitors of STS are currently being pursued in both academia and industry as potential drugs for treating breast cancer.
A series of 4-substituted estrone and estradiol derivatives were examined as inhibitors of STS. Inhibition of STS with 4-FE1, an irreversible inhibitor of STS previously studied in the Taylor group, can be enhanced by introducing a hydrophobic benzyl group at the 17-positon of 4-FE1. As with 4-FE1, the inhibition was concentration and time-dependent. Only 14% of the activity could be recovered after extensive dialysis. Introducing substituents at the 2-position of 4-formyl estrogen derivatives resulted in loss of concentration and time-dependent inhibition and a considerable decrease in inhibitor affinity. Studies with estrogen derivatives substituted at the 4-position with groups other than a formyl revealed that a relatively good reversible inhibitor can be obtained simply by introducing an electron withdrawing group at this position. These types of inhibitors are non-competitive inhibitors suggesting an alternative steroid binding site.
A series of estrone derivatives were examined as photoaffinity labels of STS. 4-azidoestrone suflate and 4-azidoestrone phosphate exhibited properties that are suitable for photoaffinity labeling studies with STS. These labels may be useful for ascertaining pathways of substrate entry into the STS active site. 16-diazoestrone phosphate was not a photoaffinity label of STS. 2- and 4-azido estrone and 16-diazoestrone all acted as photoaffinity labels of STS. These compounds may be useful for ascertaining pathways of product release from the STS active site.
|
17 |
In vitro characterization of aromatase, estrone sulfotransferase and estrone sulfatase activities in the porcine placenta and endometrium at 30, 60 and 90 days of gestationHopkins, Katherine Lee 17 November 2012 (has links)
The purpose of this investigation was to characterize the activities of three enzymes, aromatase, estrone (E1) sulfotransferase and E] sulfatase, in the porcine placenta and endometrium on d 30, 60 and 90 of gestation. These enzymes play key roles in determining in utero concentrations of estrogens. Days 30, 60 and 90 were chosen because previous investigations had determined that these were times of substantial changes in in vitro estrogen production by the porcine placenta and endometrium. / Master of Science
|
18 |
LOCALIZATION AND FUNCTIONAL CHARACTERIZATION OF OATP4C1 TRANSPORTER IN <i>IN VITRO</i> CELL SYSTEMS AND HUMAN/RAT TISSUESKuo, Kuei-Ling 01 January 2012 (has links)
The organic anion transporting polypeptide 4c1 (Oatp4c1) was previously identified as a novel uptake transporter predominantly expressed at the basolateral membrane in the rat kidney proximal tubules. Its functional role was suggested to be a vectorial transport partner of an apically-expressed efflux transporter for the efficient translocation of physiological substrates into urine, some of which were suggested to be uremic toxins. In vitro studies in polarized cell lines showed that upon transfection rat Oatp4c1 localizes at the apical membrane. The objectives of this project were to further validate the subcellular localization of Oatp4c1/OATP4C1 in rat and human tissues as well as their localization and function in polarized cells.
Using several complementary biochemical, molecular and proteomic methods as well as antibodies amenable to immunohistochemistry, immunofluorescence, and immunoblotting, we investigated the expression pattern of Oatp4c1 in epithelial cell lines and in the rat kidney and mammary gland (MG). Collectively, these data demonstrated that rat Oatp4c1 localized at the apical cell surface of polarized epithelium and primarily in the proximal straight tubules, the S3 segment of proximal tubule, in the juxtamedullary cortex.
Drug uptake studies in Oatp4c1-expressing cells demonstrated that Oatp4c1- mediated estrone-3-sulfate (E3S) uptake was ATP-independent and pH-dependent. The increased E3S transport activity at acidic extracellular pH was ascribed to the increased maximum transport rate (Vmax). In addition, E3S transport inhibition by various substrates suggests that Oatp4c1 possesses multiple substrate binding sites.
The apical localization of Oatp4c1 in the rat kidney and MG is a novel finding and implies that this transporter protein plays a role in the reabsorption, not vectorial secretion, of its substrates. In addition, the upregulation of Oatp4c1 expression during lactation indicates that it is involved in reuptake of xenobiotic from the milk, resulting in their reduced exposure to the suckling infants, or that it functions as a scavenger system. Further, studies to identify physiological substrates are needed to better understand the significance of Oatp4c1 function in renal and mammary epithelium.
|
19 |
Rapid social regulation of 3β-HSD activity in the songbird brainPradhan, Devaleena S. 11 1900 (has links)
Rapid increases in plasma androgens are generally associated with short-term aggressive challenges in many breeding vertebrates. However, some animals such as song sparrows (Melospiza melodia) are aggressive year-round, even during the non-breeding season, when gonads are regressed and systemic testosterone (T) levels are non-detectable. In contrast, levels of the prohormone dehydroepiandrosterone (DHEA) are elevated year-round in the plasma and brain. The local conversion of brain DHEA to potent androgens may be critical in regulating non-breeding aggression. 3β-hydroxysteroid dehydrogenase/Δ4-Δ5 isomerase (3β-HSD) catalyzes DHEA conversion to androstenedione (AE) and the cofactor NAD⁺ assists in this transformation. In this thesis, I asked whether brain 3β-HSD activity is regulated by social encounters in seasonally breeding male songbirds. In Experiment 1, I looked at the long-term seasonal regulation of brain 3β-HSD activity. 3β-HSD activity was highest in the non-breeding season compared to the breeding season and molt. In Experiment 2, I hypothesized that brain 3β-HSD activity is rapidly regulated by short-term social encounters during the non-breeding season. A 30 min social challenge increased aggressive behavior. Without exogenous NAD⁺, there was ~355% increase in 3β-HSD activity in the caudal telencephalon and ~615% increase in the medial central telencephalon compared to controls (p<0.05). With exogenous NAD⁺, there was no effect of social challenge on 3β-HSD activity. These data suggest that endogenous cofactors play a critical role in the neuroendocrine response to social challenges. The increase in brain DHEA conversion to AE during social challenges may be a mechanism to rapidly increase local androgens in the non-breeding season, when there are many costs of systemic T.
|
20 |
Rapid social regulation of 3β-HSD activity in the songbird brainPradhan, Devaleena S. 11 1900 (has links)
Rapid increases in plasma androgens are generally associated with short-term aggressive challenges in many breeding vertebrates. However, some animals such as song sparrows (Melospiza melodia) are aggressive year-round, even during the non-breeding season, when gonads are regressed and systemic testosterone (T) levels are non-detectable. In contrast, levels of the prohormone dehydroepiandrosterone (DHEA) are elevated year-round in the plasma and brain. The local conversion of brain DHEA to potent androgens may be critical in regulating non-breeding aggression. 3β-hydroxysteroid dehydrogenase/Δ4-Δ5 isomerase (3β-HSD) catalyzes DHEA conversion to androstenedione (AE) and the cofactor NAD⁺ assists in this transformation. In this thesis, I asked whether brain 3β-HSD activity is regulated by social encounters in seasonally breeding male songbirds. In Experiment 1, I looked at the long-term seasonal regulation of brain 3β-HSD activity. 3β-HSD activity was highest in the non-breeding season compared to the breeding season and molt. In Experiment 2, I hypothesized that brain 3β-HSD activity is rapidly regulated by short-term social encounters during the non-breeding season. A 30 min social challenge increased aggressive behavior. Without exogenous NAD⁺, there was ~355% increase in 3β-HSD activity in the caudal telencephalon and ~615% increase in the medial central telencephalon compared to controls (p<0.05). With exogenous NAD⁺, there was no effect of social challenge on 3β-HSD activity. These data suggest that endogenous cofactors play a critical role in the neuroendocrine response to social challenges. The increase in brain DHEA conversion to AE during social challenges may be a mechanism to rapidly increase local androgens in the non-breeding season, when there are many costs of systemic T.
|
Page generated in 0.0355 seconds