Ranunculus is the largest genus in the Ranunculaceae family and comprises c. 600 species. Its distribution is almost worldwide and the largest number of species occurs in temperate zones of North and South America, Europe, Asia, Australia, New Zealand, and in the alpine regions of New Guinea. In New Zealand the genus Ranunculus contains about 41 species and is found both in lowland and alpine environments. This thesis reports a phylogentic analysis of lowland and alpine New Zealand Ranunculus, an assessment of morphological variation and species boundaries among complex alpine species and examines evidence suggesting adaptive radiation of the alpine Ranunculus lineage. Phylogenetic analysis suggests that New Zealand species of Ranunculus are not a monophyletic group. For some New Zealand species the closest affinities inferred from the analysis of nrDNA and cpDNA sequences are to species from other land masses such as Australia, the Northern Hemisphere, southern South America and islands in the southern Oceans. Contrary to Fisher’s hypothesis (1965), the Andean South American Ranunculus in the section Trollianthoideae are not closely related to the New Zealand alpine group. The Trollianthoideae section was not monophyletic and the Peruvian-Ecuadorian species in it form a lineage sister to European alpine species. Instead, aquatic and sub-aquatic species from the Euro-Mediterranean region and southern South America and the Kerguelen Island were inferred as the closest relatives to the New Zealand alpine Ranunculus; albeit this relationship was weakly supported. Findings from this study suggest that colonisation of Ranunculus into the Southern Hemisphere has been a dynamic process and several long distance dispersal events and different colonisation routes have been used. Dispersal from New Zealand to Australia and vice versa, has also been inferred. Bird transportation and oceanic currents are speculated as being the most likely vectors for long dispersal for this group. Morphological variability at the species level is a feature of several species of Ranunculus worldwide. In New Zealand, the alpine species R. insignis and R. enysii are characterised by extensive morphological variability across their distribution range. Currently, these two species include a number of geographically restricted forms that in earlier taxonomic treatments were considered as separate species. Analysis of qualitative and quantitative morphological characters using parametric and non-parametric statistical tests and multivariate analysis, habitat characterisation using environmental variables from the GIS database LENZ and molecular analyses of nrDNA and cpDNA sequences have provided a framework for interpreting and understanding the nature of this phenotypic variation. An argument based on morphological, genetic and ecological support for the reinstatement of the species R. insignis, R. lobulatus and R. monroi is presented here. The last two species may correspond to lineages of recent origin. Hybridisation and introgression between R. insignis and R. lobulatus are suggested as being responsible for intermediate phenotypes found in areas where their distribution overlaps. Morphological variability in R. enysii is inferred to have had a complex origin. The species has a disjunct distribution and events of hybridisation and/or introgression with R. monroi and R. gracilipes seems to have occurred in some of the northern and southern populations, respectively. These hybrid lineages may have swamped out pure lineages of R. enysii and eliminated the ancestral phenotype. Studies including assessment of gene flow using microsatellites, phenotypic stability under common garden condition and pollination experiments will be necessary to further test these hypotheses. Contrary to the latter two species, R. lyallii is morphologically uniform across its distribution range but genetically diverse (11 haplotypes, one of them shared with R. buchananii). Morphological stability in this species is probably explained by morphological stasis and habitat specialisation. The alpine Ranunculus group is outstanding in the New Zealand flora in terms of its great phenotypic and ecological diversity of its members. These two features plus the monophyletic nature of the group and its recent origin have suggested to previous researchers that the radiation of this group has been adaptive. Phylogenetic analysis of 20 taxa in this group using nrDNA and cpDNA sequences has shown that the group includes four lineages and that genetic diversity between the species forming each linage is low. This confirms findings from earlier studies by Lockhart et al. (2001). Cluster Analysis, multidimensional scaling analysis and histological and scanning microscopy observations of morphological and anatomical vegetative and reproductive characters were used to quantify the extent of morphological diversity in the group. Habitat diversity of this group was characterised using 16 environmental variables available from the GIS database LENZ and analysed using Canonical variates analysis. Although four habitat types were identified, there was no correlation between habitat and phenotype as predicted for an adaptive radiation. A number of alternative explanations for this lack of correspondence are discussed. The conclusion drawn from this study was that available data layers and resolution of LENZ limit the use of GIS databases for testing hypotheses of adaptation in the New Zealand Alps.

The vegetation of the island of Rangitoto was examined in order to determine the current vegetation pattern and to identify the factors which have influenced the development of this pattern. Information about the order and dates of eruptive events was reviewed to gauge the length of time that the various surfaces of the island have been available as a substrate for the development of a vegetation covering. Available geological information, dating evidence, historical accounts, tree ages, and Maori evidence all point to a single period of eruptive activity, probably only several years in duration, around the mid- to late-1300's (A.D.). The order of eruption was probably production of the ash that covers neighbouring islands, followed by cinder cone building, followed by eruption of the lava flows. The lava flows were found to consist of clinkery aa flows, blocky aa flows, and a flow type referred to as Rangitoto slab flows. These flow types could be partially distinguished from one another, using discriminant analysis, on the basis of the length of the longest fragment on a 5x5m plot and the number of fragments on a lxlm subplot. A new transition sequence of flow types from pahoehoe to aa lavas is proposed for viscous lavas undergoing low rates of shear strain. The vegetation pattern of the lava fields was examine using TWINSPAN and CANOCO analysis of foliage cover percentage information gathered from 125 5x5m plots. It was found to consist of a successional sequence of vegetation arranged in a mosaic. The mosaic was found to relate strictly to the underlying lava flow surface. Large Metrosideros grow in crevices on slab flows and next to large boulders on aa flows. Smaller Metrosideros grow on small slabs on slab flows. Mixed species scrub is found growing on unbroken slab surfaces. The relative rate of colonisation of different types of flows under different climatic conditions was considered. The Metrosideros hybrid swarm was studied using morphometric information. The putative parent species of the swarm were found, using Principal Components Analysis, to be Metrosideros excelsa and Metrosideros robusta. The Rangitoto Metrosideros population was found, using Canonical Variates Analysis, to be the result of hybridisation, followed by introgression towards M. excelsa . The probable F1 hybrids grow in early successional stage vegetation. The major geographical trend is the tendency for backcrossed hybrids to grow on the eastern side of the island, suggesting eastern origins of M. robusta seeds. The impact of browsing animals on the vegetation was studied over five years in exclosures and corresponding control sites. Metrosideros foliage recovered slightly. Griselinia lucida and Cyathodes juniperina seedlings were recruited into the shrub layers in exclosures, but not in control sites. Astelia seedlings also benefitted from the absence of browsing pressure, as did Thelymitra longifolia. The distribution of browsing animal populations in relation to the vegetation pattern was studied using faecal pellet recruitment data gathered by the New Zealand Forest Service in 1984. Both wallabies (Petrogale penicillata penicillata) and possums (Trichosurus vulpecula) were found to be distributed principally according to the amount of palatable foliage available to each species in each vegetation type. / Appendices restricted at the request of the author

The vegetation of the island of Rangitoto was examined in order to determine the current vegetation pattern and to identify the factors which have influenced the development of this pattern. Information about the order and dates of eruptive events was reviewed to gauge the length of time that the various surfaces of the island have been available as a substrate for the development of a vegetation covering. Available geological information, dating evidence, historical accounts, tree ages, and Maori evidence all point to a single period of eruptive activity, probably only several years in duration, around the mid- to late-1300's (A.D.). The order of eruption was probably production of the ash that covers neighbouring islands, followed by cinder cone building, followed by eruption of the lava flows. The lava flows were found to consist of clinkery aa flows, blocky aa flows, and a flow type referred to as Rangitoto slab flows. These flow types could be partially distinguished from one another, using discriminant analysis, on the basis of the length of the longest fragment on a 5x5m plot and the number of fragments on a lxlm subplot. A new transition sequence of flow types from pahoehoe to aa lavas is proposed for viscous lavas undergoing low rates of shear strain. The vegetation pattern of the lava fields was examine using TWINSPAN and CANOCO analysis of foliage cover percentage information gathered from 125 5x5m plots. It was found to consist of a successional sequence of vegetation arranged in a mosaic. The mosaic was found to relate strictly to the underlying lava flow surface. Large Metrosideros grow in crevices on slab flows and next to large boulders on aa flows. Smaller Metrosideros grow on small slabs on slab flows. Mixed species scrub is found growing on unbroken slab surfaces. The relative rate of colonisation of different types of flows under different climatic conditions was considered. The Metrosideros hybrid swarm was studied using morphometric information. The putative parent species of the swarm were found, using Principal Components Analysis, to be Metrosideros excelsa and Metrosideros robusta. The Rangitoto Metrosideros population was found, using Canonical Variates Analysis, to be the result of hybridisation, followed by introgression towards M. excelsa . The probable F1 hybrids grow in early successional stage vegetation. The major geographical trend is the tendency for backcrossed hybrids to grow on the eastern side of the island, suggesting eastern origins of M. robusta seeds. The impact of browsing animals on the vegetation was studied over five years in exclosures and corresponding control sites. Metrosideros foliage recovered slightly. Griselinia lucida and Cyathodes juniperina seedlings were recruited into the shrub layers in exclosures, but not in control sites. Astelia seedlings also benefitted from the absence of browsing pressure, as did Thelymitra longifolia. The distribution of browsing animal populations in relation to the vegetation pattern was studied using faecal pellet recruitment data gathered by the New Zealand Forest Service in 1984. Both wallabies (Petrogale penicillata penicillata) and possums (Trichosurus vulpecula) were found to be distributed principally according to the amount of palatable foliage available to each species in each vegetation type. / Appendices restricted at the request of the author

Phosphate (P) supply is one of the major determining factors to plant productivity, since the element affects the growth and the development of plants. In response to Pdeficiency treatment, plants display alterations in root system architecture caused by changes in primary root (PR) and lateral root (LR) length and LR density. In this thesis, the root growth of the agronomically important legume, white clover (Trifolium repens L.) was found to be slightly stimulated in terms of PR length, LR number and total LR length when plants were grown in a P-deficient media (0.01 mM orthophosphate; Pi) when compared with plants grown in a P-sufficient media (1.00 mM Pi) when using a hydroponic growth system. When plants are grown in a P-sufficient media, treatment with 100 nM exogenous 1- aminocyclopropane-1-carboxylic acid (ACC) and exogenous auxin (5 nM 1- naphthylacetic acid, NAA) resulted in significant increases in white clover PR length, LR number and LR length. However, when ethylene action or auxin transport were inhibited using 300 ppm 1-methylcyclopropene (1-MCP) and 100 nM 1-Nnaphthylphthalamic acid (NPA), respectively, root growth was significantly reduced which suggests roles for ethylene and auxin in mediating white clover root growth. To examine the effects of these hormones on plants grown in P-deficient media, 100 nM ACC treatment significantly enhanced the stimulatory effects of growth on Pdeficient media only, while exposure of plants to P-deficiency alone was sufficient to significantly neutralise the inhibitory effects of 1-MCP on root growth. Hence, exposure to P-deficiency is proposed to increase either ethylene biosynthesis or ethylene sensitivity in white clover roots. In contrast, for plants grown in P-deficient media, treatment with 5 nM NAA significantly abolished the stimulation of white clover root growth observed with P-deficiency so it is proposed that exposure to Pdeficiency increases either auxin biosynthesis or auxin sensitivity, but the 5nM NAA concentration used was too high to stimulate root growth. Using DR5p::GUS transgenic white clover, auxin activity was found in the root tips and root primordia. Using these plants, it is suggested that P-deficient treatment and ACC treatment influenced white clover root growth through an increase in auxin sensitivity. Overall, ethylene and auxin are found to be essential in mediating white clover root growth in P-sufficiency, and also in mediating root responses to P-deficiency through changes in terms of the biosynthesis and the sensitivity of these two hormones.

Phosphate (P) supply is one of the major determining factors to plant productivity, since the element affects the growth and the development of plants. In response to Pdeficiency treatment, plants display alterations in root system architecture caused by changes in primary root (PR) and lateral root (LR) length and LR density. In this thesis, the root growth of the agronomically important legume, white clover (Trifolium repens L.) was found to be slightly stimulated in terms of PR length, LR number and total LR length when plants were grown in a P-deficient media (0.01 mM orthophosphate; Pi) when compared with plants grown in a P-sufficient media (1.00 mM Pi) when using a hydroponic growth system. When plants are grown in a P-sufficient media, treatment with 100 nM exogenous 1- aminocyclopropane-1-carboxylic acid (ACC) and exogenous auxin (5 nM 1- naphthylacetic acid, NAA) resulted in significant increases in white clover PR length, LR number and LR length. However, when ethylene action or auxin transport were inhibited using 300 ppm 1-methylcyclopropene (1-MCP) and 100 nM 1-Nnaphthylphthalamic acid (NPA), respectively, root growth was significantly reduced which suggests roles for ethylene and auxin in mediating white clover root growth. To examine the effects of these hormones on plants grown in P-deficient media, 100 nM ACC treatment significantly enhanced the stimulatory effects of growth on Pdeficient media only, while exposure of plants to P-deficiency alone was sufficient to significantly neutralise the inhibitory effects of 1-MCP on root growth. Hence, exposure to P-deficiency is proposed to increase either ethylene biosynthesis or ethylene sensitivity in white clover roots. In contrast, for plants grown in P-deficient media, treatment with 5 nM NAA significantly abolished the stimulation of white clover root growth observed with P-deficiency so it is proposed that exposure to Pdeficiency increases either auxin biosynthesis or auxin sensitivity, but the 5nM NAA concentration used was too high to stimulate root growth. Using DR5p::GUS transgenic white clover, auxin activity was found in the root tips and root primordia. Using these plants, it is suggested that P-deficient treatment and ACC treatment influenced white clover root growth through an increase in auxin sensitivity. Overall, ethylene and auxin are found to be essential in mediating white clover root growth in P-sufficiency, and also in mediating root responses to P-deficiency through changes in terms of the biosynthesis and the sensitivity of these two hormones.

The Grasslands II perennial ryegrass (Lolium perenne L.) mapping population comprising F1 progeny with the two parents (one plant each from the cultivars Samson and Impact) (Crush et al., 2007) was used to detect putative quantitative trait loci (QTL) for leaf Rubisco turnover and herbage yield traits. Rubisco turnover was described by three mathematical parameters: d (maximum Rubisco content), g (time of d) and f (a measure of curve width). All three parameters exhibited continuous variation among the F1 progeny. Sixteen QTL were detected, seven for Rubisco turnover and nine for herbage yield traits. Support interval overlap on linkage group (LG) 1 and close location on LG2 for plant dry weight (DW) QTL in this study and in a previous analysis (Sartie, 2007) of the same mapping population suggests DW QTL stability across environments. Some QTL identified by Sartie (2007) were not re-confirmed in this study, but new QTL were identified. This suggests genotype x environment interaction generated by variable expression of genes in different environments. Clusters of QTL with overlapping support intervals were found on LG2 and LG5. The cluster on LG2 included QTL for herbage yield traits leaf lamina length (LL), tiller number (TN), productivity index (PI) and DW. The cluster on LG5 included QTL for DW, PI, TN, and d. These two regions offer potential for plant breeding applications. Apart from the QTL for d on LG5, there was no co-location of Rubisco turnover and herbage yield QTL. However, principal component analysis indicated plants with lower d tended to have higher DW; thus Rubisco turnover effects on plant productivity may relate to energy cost of Rubisco synthesis rather than photosynthetic capacity. DW was generally unrelated to f and g; therefore, hypothesised nitrogen use inefficiencies arising from premature Rubisco degradation, or retention of Rubisco at leaf senescence, were not confirmed. LG5 and LG7 on which QTL for d were located have conserved syntenic regions with rice chromosomes 8 and 9 where QTL for Rubisco content at different stages during heading were mapped by Ishimaru et al (2001a).

Content removed due to copyright restrictions: Taylor, C. H., & Long, P. G. (2000). Review of literature on camellia flower blight caused by ciborinia camelliae. New Zealand Journal of Crop and Horticultural Science, 28(2), 123-138. / Camellias are popular ornamental plants and the most serious pathogen of this plant is camellia flower blight, caused by the fungal pathogen Ciborinia camelliae Kohn. Ascospores of this fungus attack the flowers, turning them brown, rendering infected flowers unattractive. Little is known about the pathogen and control measures are not particularly effective. In this thesis, various aspects of the pathogen's basic and molecular biology and interaction with host species were studied. Surveys of the distribution and spread of C. camelliae within New Zealand determined that the pathogen was present in most regions of the North Island, and north and east coasts of the South Island. Over the distances and time involved, it appeared that the disease was spreading mainly by windborne ascospores rather than human transfer. Sclerotia were germinated out of season to increase the period during which ascospores were available for infection work. Greatest germination was achieved at low temperatures (5°C-10°C) in 24 h darkness. Isolate-specific primers were designed to the ribosomal DNA Internal Transcribed Spacer region to detect the pathogen in planta and distinguish between New Zealand isolates of C. camelliae and other fungal pathogens. Phylogenetic analysis of the ITS region with other Ciborinia, Sclerotinia and Botrytis species showed that C. camelliae was more closely related to S. sclerotiorum than other Ciborinia species. Two inoculation techniques for infecting Camellia petals with ascospores of C. camelliae were developed and tested. Inoculation using airborne ascospores in a settling chamber was a simple and quick method for testing large numbers of species for resistance. Inoculation of ascospores in suspension produced qualitative data, but was more time consuming. Of the four mechanisms of resistance tested, levels of aluminium hyperaccumulation and the presence of phenolic compounds did not correlate with resistance in Camellia species. The large uptake of aluminium, however, did indicate that Camellia species would be good plants for phytoremediation of acid soils. Some resistant species were found to have cell wall modifications and/or lignification of cell walls in response to C. camelliae infection and chitinase activity was found in most resistant Camellia species tested. Further research into these latter two mechanisms is recommended and indicates that the development of resistant Camellia cultivars is possible.

The results are presented of eight experiments designed to investigate the influence of interrelationships between bud population dynamics and carbohydrate supply from root stores on spear production in asparagus (Asparagus officinalis L). These investigations involved studies in the field and the greenhouse, and using aeroponics and hydroponics techniques to facilitate non-destructive studies of plant development. The evidence indicated that spear yield was limited by the number of buds of adequate size for developing into marketable spears, rather than total bud number. It was shown that bud development continues throughout the harvest period. About 14% of these buds contributed to fern production after harvest, but the majority were involved, following a period of dormancy, in development of the next season's spears. Approximately 16% of the new buds contributed to spear yield in the current harvest, 68% were dormant until the following summer and contributed to 18% of total buds at that time. Spear production was most efficient in plants with large crowns, since the effects of correlative inhibition on spear development were greater in small than large crowns. Nevertheless, increase in crown size in terms of root mass is not necessarily accompanied by an equivalent increase in bud number or cluster number, and bud availability is potentially an important yield limiting factor. However, large crowns reduced the period of correlative inhibition within a bud cluster. Crown size and bud population were sensitive to nutrient supply, and it is suggested that control of nutrient supply over the harvest period may be best achieved by use of slow-release fertilizer or split application of nitrogen. Carbohydrate partitioning and possibly photosynthetic rate were also sensitive to daylength, and there was some evidence of genotypic variation in the response to daylength changes and contrasts. Principal component analysis indicated that numbers of buds and bud clusters, plant size and chlorophyll content were the main determinants of spear yield, and cluster analysis demonstrated potentially important genetic variation for these variables in potentially high yielding cultivars. Spear yield is the product of harvest intensity and harvest duration, and harvest duration itself was shown to be sensitive to genotype and management effects on bud initiation and development. A conceptual model is used to illustrate the influence of bud population and bud cluster characteristics on harvest intensity and duration, and on spear yield, and the relative importance of management manipulation of bud dynamics and carbohydrate supply to spear yield.

The vegetation of the island of Rangitoto was examined in order to determine the current vegetation pattern and to identify the factors which have influenced the development of this pattern. Information about the order and dates of eruptive events was reviewed to gauge the length of time that the various surfaces of the island have been available as a substrate for the development of a vegetation covering. Available geological information, dating evidence, historical accounts, tree ages, and Maori evidence all point to a single period of eruptive activity, probably only several years in duration, around the mid- to late-1300's (A.D.). The order of eruption was probably production of the ash that covers neighbouring islands, followed by cinder cone building, followed by eruption of the lava flows. The lava flows were found to consist of clinkery aa flows, blocky aa flows, and a flow type referred to as Rangitoto slab flows. These flow types could be partially distinguished from one another, using discriminant analysis, on the basis of the length of the longest fragment on a 5x5m plot and the number of fragments on a lxlm subplot. A new transition sequence of flow types from pahoehoe to aa lavas is proposed for viscous lavas undergoing low rates of shear strain. The vegetation pattern of the lava fields was examine using TWINSPAN and CANOCO analysis of foliage cover percentage information gathered from 125 5x5m plots. It was found to consist of a successional sequence of vegetation arranged in a mosaic. The mosaic was found to relate strictly to the underlying lava flow surface. Large Metrosideros grow in crevices on slab flows and next to large boulders on aa flows. Smaller Metrosideros grow on small slabs on slab flows. Mixed species scrub is found growing on unbroken slab surfaces. The relative rate of colonisation of different types of flows under different climatic conditions was considered. The Metrosideros hybrid swarm was studied using morphometric information. The putative parent species of the swarm were found, using Principal Components Analysis, to be Metrosideros excelsa and Metrosideros robusta. The Rangitoto Metrosideros population was found, using Canonical Variates Analysis, to be the result of hybridisation, followed by introgression towards M. excelsa . The probable F1 hybrids grow in early successional stage vegetation. The major geographical trend is the tendency for backcrossed hybrids to grow on the eastern side of the island, suggesting eastern origins of M. robusta seeds. The impact of browsing animals on the vegetation was studied over five years in exclosures and corresponding control sites. Metrosideros foliage recovered slightly. Griselinia lucida and Cyathodes juniperina seedlings were recruited into the shrub layers in exclosures, but not in control sites. Astelia seedlings also benefitted from the absence of browsing pressure, as did Thelymitra longifolia. The distribution of browsing animal populations in relation to the vegetation pattern was studied using faecal pellet recruitment data gathered by the New Zealand Forest Service in 1984. Both wallabies (Petrogale penicillata penicillata) and possums (Trichosurus vulpecula) were found to be distributed principally according to the amount of palatable foliage available to each species in each vegetation type. / Appendices restricted at the request of the author

Phosphate (P) supply is one of the major determining factors to plant productivity, since the element affects the growth and the development of plants. In response to Pdeficiency treatment, plants display alterations in root system architecture caused by changes in primary root (PR) and lateral root (LR) length and LR density. In this thesis, the root growth of the agronomically important legume, white clover (Trifolium repens L.) was found to be slightly stimulated in terms of PR length, LR number and total LR length when plants were grown in a P-deficient media (0.01 mM orthophosphate; Pi) when compared with plants grown in a P-sufficient media (1.00 mM Pi) when using a hydroponic growth system. When plants are grown in a P-sufficient media, treatment with 100 nM exogenous 1- aminocyclopropane-1-carboxylic acid (ACC) and exogenous auxin (5 nM 1- naphthylacetic acid, NAA) resulted in significant increases in white clover PR length, LR number and LR length. However, when ethylene action or auxin transport were inhibited using 300 ppm 1-methylcyclopropene (1-MCP) and 100 nM 1-Nnaphthylphthalamic acid (NPA), respectively, root growth was significantly reduced which suggests roles for ethylene and auxin in mediating white clover root growth. To examine the effects of these hormones on plants grown in P-deficient media, 100 nM ACC treatment significantly enhanced the stimulatory effects of growth on Pdeficient media only, while exposure of plants to P-deficiency alone was sufficient to significantly neutralise the inhibitory effects of 1-MCP on root growth. Hence, exposure to P-deficiency is proposed to increase either ethylene biosynthesis or ethylene sensitivity in white clover roots. In contrast, for plants grown in P-deficient media, treatment with 5 nM NAA significantly abolished the stimulation of white clover root growth observed with P-deficiency so it is proposed that exposure to Pdeficiency increases either auxin biosynthesis or auxin sensitivity, but the 5nM NAA concentration used was too high to stimulate root growth. Using DR5p::GUS transgenic white clover, auxin activity was found in the root tips and root primordia. Using these plants, it is suggested that P-deficient treatment and ACC treatment influenced white clover root growth through an increase in auxin sensitivity. Overall, ethylene and auxin are found to be essential in mediating white clover root growth in P-sufficiency, and also in mediating root responses to P-deficiency through changes in terms of the biosynthesis and the sensitivity of these two hormones.