No description available.

Human mammary tissue was incubated in vitro with [7-3H] dehydroepiandrosterone sulphate (DHA-sulphate) and in agreement with other investigators this steroid conjugate was metabolized to DHA and other steroid products. Sulphatase activity was greater in the malignant than the non-malignant tissues and was found to be a function of the tissue cellularity. One of the major products, a "polar steroid" necessitated identification. The "polar steroid" was identified principally as 7a-hydroxy DHA by chemical modification techniques and co-crystallization of the purified steroid metabolite with carrier 7a-hydroxy DHA. This carrier required synthesis and characterization.

Human mammary tissue was incubated in vitro with [7-3H] dehydroepiandrosterone sulphate (DHA-sulphate) and in agreement with other investigators this steroid conjugate was metabolized to DHA and other steroid products. Sulphatase activity was greater in the malignant than the non-malignant tissues and was found to be a function of the tissue cellularity. One of the major products, a "polar steroid" necessitated identification. The "polar steroid" was identified principally as 7a-hydroxy DHA by chemical modification techniques and co-crystallization of the purified steroid metabolite with carrier 7a-hydroxy DHA. This carrier required synthesis and characterization.

Human mammary tissue was incubated in vitro with [7-3H] dehydroepiandrosterone sulphate (DHA-sulphate) and in agreement with other investigators this steroid conjugate was metabolized to DHA and other steroid products. Sulphatase activity was greater in the malignant than the non-malignant tissues and was found to be a function of the tissue cellularity. One of the major products, a "polar steroid" necessitated identification. The "polar steroid" was identified principally as 7a-hydroxy DHA by chemical modification techniques and co-crystallization of the purified steroid metabolite with carrier 7a-hydroxy DHA. This carrier required synthesis and characterization.

Human mammary tissue was incubated in vitro with [7-3H] dehydroepiandrosterone sulphate (DHA-sulphate) and in agreement with other investigators this steroid conjugate was metabolized to DHA and other steroid products. Sulphatase activity was greater in the malignant than the non-malignant tissues and was found to be a function of the tissue cellularity. One of the major products, a "polar steroid" necessitated identification. The "polar steroid" was identified principally as 7a-hydroxy DHA by chemical modification techniques and co-crystallization of the purified steroid metabolite with carrier 7a-hydroxy DHA. This carrier required synthesis and characterization.

Human mammary tissue was incubated in vitro with [7-3H] dehydroepiandrosterone sulphate (DHA-sulphate) and in agreement with other investigators this steroid conjugate was metabolized to DHA and other steroid products. Sulphatase activity was greater in the malignant than the non-malignant tissues and was found to be a function of the tissue cellularity. One of the major products, a "polar steroid" necessitated identification. The "polar steroid" was identified principally as 7a-hydroxy DHA by chemical modification techniques and co-crystallization of the purified steroid metabolite with carrier 7a-hydroxy DHA. This carrier required synthesis and characterization.

Human mammary tissue was incubated in vitro with [7-3H] dehydroepiandrosterone sulphate (DHA-sulphate) and in agreement with other investigators this steroid conjugate was metabolized to DHA and other steroid products. Sulphatase activity was greater in the malignant than the non-malignant tissues and was found to be a function of the tissue cellularity. One of the major products, a "polar steroid" necessitated identification. The "polar steroid" was identified principally as 7a-hydroxy DHA by chemical modification techniques and co-crystallization of the purified steroid metabolite with carrier 7a-hydroxy DHA. This carrier required synthesis and characterization.