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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

Motor cooperation in bi-directional early endosome motility

Schuster, Martin 2011 (has links)
In mammalian cells and fungi, early endosomes form a dynamic compartment that undergoes bi-directional motility along microtubules. Previous work has shown that in the model system Ustilago maydis early endosome motility involves the opposing motor proteins dynein and kinesin-3. Here I performed a detailed analysis of the role of the motors in early endosome motility, using quantitative live cell imaging of kinesin-3, dynein and the endosomal GTPase Rab5a. In the first part of my work, I analysed the role of dynein at MT plus-ends, where the motor forms a strong accumulation that was thought to be involved in capturing early endosomes. I could demonstrate that ~55 dynein motors build up the dynein accumulation. In collaboration with Ms. Congping Lin and Prof. Peter Ashwin (Institute for Mathematics, Exeter), I found theoretical evidence that ~25 dynein motors concentrate and leave the plus-ends stochastically. In addition, dynein motors are captured by an interaction of dynactin and the plus-end binding protein EB1. Together both mechanisms increase the number of motors, which ensures that EEs will be loaded onto dynein before they reach the end of their track. In a second project, I provide evidence that loading of dynein is not restricted to the plus-ends. Instead, dynein leaves the plus-ends and is able to bind to kinesin-3 delivered early endosomes, which changes their transport direction from anterograde to retrograde. Kinesin-3 remains bound to these retrograde EEs. When dynein leaves the organelle, it switches back to anterograde motility. Interestingly, a single dynein wins over three to five kinesin-3 motors. I discuss these findings in the light of current motor cooperation concepts. In a third part, I demonstrated that kinesin-3 has an unexpected role in long-range retrograde endosome motility. In contrast, dynein is only responsible for the distal 10-20 µm. This is possible because most of the hyphal cells contain a symmetric and bi-polar MT array. This MT organization is reminiscent of that in dendrites. Kinesin-3-based retrograde motility is required to mix the organelles and might support long-range communication between both cell poles.

Synthesis of ligands for the large conductance calcium activated potassium channel (BK←c←a)

Power, Eoin Christopher 2001 (has links)
No description available.

Identification of amino acid residues of the NR2A subunit that control glutamate potency in recombinant NR1/NR2A NMDA receptors

Chen, Philip Eng-Chi 2000 (has links)
No description available.

The role of 5-HT←1←a receptors in the control of cardiorespiratory reflexes

Skinner, Matthew Richard Alistair 1999 (has links)
No description available.

Identification of assembly determinants in GABA←A receptor subunits

Taylor, Pamela Mary 1998 (has links)
No description available.

Ubiquinone binding sites of mitochondrial bc←1 complex

Akinsiku, Akinyemi Olutosin 2001 (has links)
No description available.

The agonist binding site of muscarinic receptors probed by scanning mutagenesis

Allman, Karen 1999 (has links)
No description available.

Amaryllidaceae alkaloids : some synthetic studies

Evans, J. R. 1971 (has links)
No description available.

Studies in natural products

Dagli, Selma 2002 (has links)
No description available.

An investigation of homo and heterodimerization of the human delta opioid receptor

McVey, Mary 2001 (has links)
No description available.

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