- About
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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
provided by the
Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
Search results
Showing 1 to 10 of 56 (0.01 seconds)| 1 |
Molecular and biochemical characterisation of cotranscriptional cleavageTeixeira, Alexandre Rui de Sampaio January 2006 (has links)
No description available.
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Quantitative phosphoproteomics of protein isoforms involved in EGF signallingGuerrera, Ida Chiara January 2005 (has links)
This thesis describes the development of a new, powerful concept and approach to study signalling pathways based on top-down quantitative phosphoproteomics. This approach, called the Phospho-SILAC strategy, combines a first step of in-house developed phosphoprotein enrichment using Immobilized Affinity Metal Chromatography (IMAC), and Stable Isotope Labelling in Cell Culture (SILAC) with labelled arginine containing six 13C atoms. NRK49F cells grown in medium containing 13C6 Arg (heavy arginine, hR) were stimulated for short times with EGF and the cell lysate obtained was mixed in a ratio 1:1 with the lysate from unlabelled cells, used as control. Phosphoproteins were then enriched from the protein mixture using IMAC and displayed on 2D gels, allowing multiple isoform separation. Individual post-translational changes in different proteins and in different isoforms of the same protein upon EGF stimulation are described. Major focus was given to Hsp8 isoforms, as they were found to be differentially involved in EGF signalling when tested with antibodies against MAPK/CDK and PCK substrates. A potential MAPK phosphorylation site on Hsp8 N-terminal sequence was identified. The Phospho-SILAC strategy was also applied to Rho and Rab GTPases and their corresponding GDI (guanidine nucleotide dissociation inhibitor) molecules. Phosphorylation might regulate the binding between GTPase-GDI. The results obtained also suggested that RhoGDI-1 activity could be regulated by arginine methylation. A potent new concept and methodology is outlined for wider investigations of molecular dynamics and for guiding the selection of candidate proteins and their modification for further studies.
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Regulation of MC2-R expression in the murine adipocyteNoon, Luke Adam January 2005 (has links)
No description available.
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Regulation of SNF1 and characterisation of its role in transcriptional controlLeiper, Fiona Callander January 2003 (has links)
No description available.
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In vitro assembly of the methanococcus jannaschii RNA polymeraseWerner, Finn January 2003 (has links)
No description available.
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An investigation of alternative isoforms of the transcription factor PAX3Wang, Qiuyu January 2005 (has links)
No description available.
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The molecular basis of functional diversity in the Aux/IAA gene family in Arabidopsis thalianaReadshaw, Anne Elizabeth January 2006 (has links)
No description available.
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RNA polymerase II during transcript elongation : dealing with DNA damage and staying phosphorylatedKong, Stephanie Ellen January 2001 (has links)
No description available.
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Sequence specific interactions, intracellular distribution and effects on transcription of novel DNA minor groove-binding polyamidesO'Hare, Celia Caroline January 2004 (has links)
No description available.
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Regulation of SRF activitySmith, Gemma Ann January 2003 (has links)
No description available.
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