MSCAGR (Plant Production) / Department of Plant Production / Colophospermum mopane (Fabaceae), commonly known as mopane tree, is an indigenous tree species in Southern Africa, often being the dominant species in semi-arid areas. It plays an integral part in the improvement of communities’ livelihoods when it is harvested for firewood and mopane worms which are edible insects are collected from the tree. Colophospermum mopane is host to many insects, among them Retroacizzia mopani, a leaf pest. Retroacizzia mopani produces lerps which are protective exudates that shield the insect from predation, but lerps also reduce the photosynthetic area of mopane leaves. This study aimed to determine the distribution of the African mopane psyllid, R. mopani and its natural enemies in the Tshikundamalema area, in Limpopo Province. The study assessed the presence of lerps on C. mopane, as well as determining the effect of site, tree height, tree density, tree position, branch size and neighbouring trees on R. mopani infestations at three sites. Sample of leaves infested with R. mopani were collected at six different sites and reared in the laboratory to determine the prevalence of R. mopani natural enemies and the levels of parasitism. The site, tree density, tree position, and branch size had an effect on the R. mopani infestations. The results showed that as the tree density, tree height and branch size increased, R. mopani infestation also increased. Neighbouring trees had no effect on R. mopani infestations. Trees found at the edge of the forest were more heavily infested compared to those that were inside the forest. All the three sites had low levels of parasitism by the natural enemy, Psyllaephagus arytainae Prinsloo. This indicates that P. arytainae has little effect on population dynamics of the psyllids.