Global ETD Search

1	Studies on porcine cytomegalovirus relevant to xenotransplantation Fryer, Jacqueline Fiona Lorna January 2005 (has links) Xenotransplantation of porcine organs into humans provides a potential solution to the current shortage of human donor organs, but raises considerable safety concerns including the potential transmission of porcine infectious agents, particularly viruses, to humans. Porcine cytomegalovirus (PCMV) is a betaherpesvirus, which causes generalised infection in newborn piglets. It is endemic in world pig populations including those of high health status, and is transmitted horizontally and in utero. PCMV represents a potential risk in xenotransplantation since its human counterpart, HCMV, is frequently transmitted from the organ donor and reactivates posttransplant, where it is associated with end-organ disease and graft rejection. Qualitative and quantitative PCR assays were developed in order to investigate the quantity and organ distribution of PCMV in pigs being bred for xenograft organs, and to investigate when virus is acquired. PCMV was found to be a common infection in conventionally-reared CD55 transgenic Large White pigs, with virus detected in potential xenograft organs of adult pigs, and widely disseminated in tissues of 3-5 week-old piglets. Prospective monitoring of piglets from birth showed that virus was acquired post-natally, whilst no evidence of in utero infection was found in caesarean-derived, barrier-reared piglets. Although these results showed that PCMV could be eliminated from pigs being bred for xenograft organs, knowledge of antiviral susceptibility is essential as it would provide therapeutic options should clinical trials proceed. The effect of antiherpetics on the growth of PCMV in a pig fallopian tube cell line was determined by real-time PCR and indirect immunofluorescence (IIF). In this cell culture model, ganciclovir and cidofovir were the most effective against PCMV replication, however, some cytotoxicity was associated with the highest concentration of cidofovir tested. Finally, a recombinant protein-based serological assay for PCMV was developed, utilising PCMV glycoprotein B expression in a baculovirus system. 617.954
2	Development potential of human haematopoietic stem cells in the NOD/Scid xenotransplantation model Kashofer, Karl January 2006 (has links) No description available. 617.954
3	Costimulation blockade and selective depletion of activated T cells : therapeutic potential in transplantation Gardner, Nicola Jane January 2006 (has links) No description available. 617.954
4	T cell costumulation in transplantation : role of the allograft endothelium Denton, Mark Donald January 2002 (has links) No description available. 617.954
5	A study of natural killer cells in allogeneic solid organ transplantation Popoola, Joyce January 2006 (has links) No description available. 617.954
6	Stem cell graft effects following unilateral cholinergic depletion De Oliveria, Alcyr Alves January 2003 (has links) No description available. 617.954
7	The role of graft expressed Fasligand in graft rejection Dooldeniya, Mohanta Deevan January 2005 (has links) No description available. 617.954
8	Studies on regulation of alloreactivity by human CD4+ CD25+ regulatory T cells Notley, Clare Angela January 2006 (has links) No description available. 617.954
9	Antibody-induced endothelial cell cytoprotection mediated through adenosine receptors : a mechanism for transplant accommodation Delikouras, Alexandros January 2005 (has links) No description available. 617.954
10	Isolating the pro-apoptotic effect of Fas ligand in transplantation Dupont, Peter John January 2006 (has links) No description available. 617.954

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