Fire has been a major cause of ship's accidents throughout maritime history. It is by far the most serious threat to life and the environment as passenger ships get larger and more sophisticated. It is also impossible to protect a passenger vessel against all hazards. Despite the fact that a passenger ship contains potential fire hazards in the engine room space, accommodation zone and electrical systems, etc, the single most important fire hazard onboard a ship may be the man himself, either unintentionally or intentionally. 'Fire safety on passenger vessel' has continued to be the focus of attention on passenger ships. The work described in this thesis is concerned with the application of Formal Fire Safety Assessment to passenger ships. The traditional way of conducting a Formal Safety Assessment (FSA) employs typical fire safety analysis methods that require a certain amount of data. Most fire accident data available for passenger vessels is associated with a high degree of uncertainty and considered to be unreliable. As such, the research carried out in this thesis is directed at the development of novel fire safety analysis methods to address this problem. This thesis proposed several subjective fire safety analysis methods for passenger vessels within the FSA methodology. Also, it concentrates on developing an advanced approach for passenger ships. A few novel safety analysis and synthesis methodologies are presented to integrate fire safety assessment with decision-making techniques so that fire safety can be taken into account from the concept design /operation stages of passenger ships. This is to ensure a more controlled development process permitting decisions regarding design and operation to be made based on fire safety assessment. Finally, this thesis is concluded by summarising the results of this research project and the areas where further effort is required to improve the developed methodologies.