This study utilized an experimental design testing low, medium, and high intensity warm-up protocols for NCAA Division I 800-meter male and female middle distance runners to determine which result in optimal outcomes in a peak performance, i.e., 800-meter time trial. Mississippi State University student athletes on the Track and Field Team who compete in middle distance running events participated. Among males, results of the repeated measures ANOVA found no significant difference among the three warm-up protocols for the first 400 meters completed. For females, repeated measures ANOVA results found no significant difference among the protocols for the first 200 meters completed. However, at the completion of the 800-meter time trial, a statistically significant difference (p < 0.05) was seen among both males and females; post hoc analyses indicated that the high intensity warm-up group had a statistically significantly lower (i.e., better) total time than the low intensity warm-up group.