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This thesis consists of a transcript and edition of Fynes Moryson's unpublished Itinerary c.1617 - 1625, with introduction, text, annotations, bibliography and index. Moryson was a gentleman traveller whose accounts of journeys undertaken in the 1590s across much of Europe as far as the Holy Land in the Ottoman Empire provide contemporary evidence of secular and religious institutions, ceremonies, customs, manners, and national characteristics. The first part of Moryson's Itinerary was published in 1617. Some of the second part was transcribed in 1903 by Charles Hughes as Shakespeare's Europe, but this is the first transcript and edition of the whole manuscript. The work has involved investigation of the historical, classical and geographical sources available to Moryson, of Elizabethan secretary hand, and of travel writing as a form of primitive anthropology. Moryson emerges as a subjective observer full of the political and religious preconceptions of the age, capable of acute insight but often unsystematic and unscientific in the assembly and presentation of his information.