Informal settlements are a relatively new phenomenon in Yemen, first documented in the 1980s (El-Shorbagi, 2008; 2007). They have since grown at a very rapid rate. Sana'a City, the nation's capital, alone has an estimated 35 informal settlements that together contain 20.5 percent of that urban center's population (El-Shorbagi, 2008; 2007). To date, the Yemeni government has paid limited attention to informal settlements. The government has not developed any specific planning policies to address their needs, partly due to meager resources and professional capacities, and partly as a consequence of conflicting (and higher priority) needs (World Bank, 2010a). The unchecked growth of informal settlements has alarmed local and national authorities as well as international organizations and recently caused officials in these entities to begin to consider seriously how to address this new community reality. This dissertation explores the engagement of state and non-state actors in informal settlement governance in Sana'a. The analysis offered here employs Mahwa Aser, the largest and most controversial informal settlement in Sana'a, as an exemplar for a broader set of concerns for all of Sana'a\s informal communities. The dissertation provides a nuanced portrait of Yemeni government capacities, policies, and practices related to Sana'a's informal settlements generally and to Mahwa Aser particularly via the perspectives and activities of multiple stakeholders, including, importantly, the community's residents. It explores the active governance roles of non-governmental and international organizations seeking to provide services in these communities as well. It also explores ways to build informal community residents' capacities to work with government and with non-governmental organizations (NGOs), international non-governmental organizations (INGOs), and international governmental organizations (IGOs) to address their many basic needs. The analysis draws on personal interviews with key stakeholders, including long-time residents of Mahwa Aser, responsible government officials, and relevant leaders of NGOs, INGOs, and IGOs in Yemen, U.S.A., and Egypt. The author also examined government and international organization reports and documents to gain insight into the governance challenges linked to continued growth of informal communities in Yemen. The study identifies a number of factors that have led to worsening living conditions in Mahwa Aser and other informal settlements in Sana'a. Taken together they suggest the Yemeni government and its partners may need to work far more self-consciously with informal community residents to establish shared goals and clear expectations. Those entities engaged collectively in governing these communities in Sana'a and in Yemen more generally will need to develop reliable policies and coherent programs within a transparent governance framework if the very difficult living conditions in such communities are to be improved. In particular, governance actors will need to devise ways and means to develop government capacities and resources even as they work to address community infrastructure and service needs in a sometimes daunting socio-cultural and economic context. / Ph. D.

Die Haplotypspezifische Extraktion (HSE) bietet für die Analyse von männlichen Mischprofilen einen neuen und direkteren Lösungsansatz, in dem die haploiden Y-chromosomalen DNS-Komponenten der einzelnen Individuen bereits vor der Analyse der individual spezifischen Marker separiert werden können und dadurch eine wirkliche physische Trennung erreicht wird. Die HSE verwendet Y-chromosomalen SNPs für die Erstellung allelspezifische Extraktionssonden, die nun gezielt nur die Marker der extrahierten DNS Komponente bzw. einer Person separieren sollen. Dabei werden im Hybridisationsschritt der HSE selektive nur komplett hybridisierte Sonden durch eine Polymerase verlängert. Während der Elongation erfolgt eine Biotinylierung des neu entstehenden Stranges, welcher dann selektiv durch Streptavidin markierte Eisenkügelchen extrahiert werden kann. Erste Durchführungen einer HSE zeigten nur eine sehr schwache bis keine Anreicherung. Während der Optimierung verschiedener Parameter wurde die Schlüsselstellung des Sondendesigns in der HSE-Technik deutlich. Die Ergebnisse zeigten, dass die neu entwickelten Sonden den Trennungserfolg der Mischprobe enorm verbessern und in einigen Fällen sogar zum Ausschluss des konkurrierenden Allels führten. Ein Vergleich der HSE Ergebnisse mit den simulierten Sondenparametern der getesteten Sonden ergab, dass der Extraktionserfolg der Sonde maßgeblich durch das Zusammenspiel von Sondenlänge und GC-Gehalt bestimmt wird. Durch dieses neu gewonnene Verständnis über den Einfluss der einzelnen Sondenparameter auf den Trennungserfolg der Mischprobe, können für künftige HSE Anwendungen Sonden effizienter erstellt und deren Wirksamkeit vorhergesagt werden. Zusätzlich konnte das neu entwickelte Vorhersage-Model der Sondenspezifität auch für weitere Extraktionsorte außerhalb des Y-Chromosoms bestätigt werden. Weiterhin konnte durch die Kombination verschiedener Sonden in einer Multiplex HSE mehrerer Y-chromosomaler Marker gleichzeitig getrennt. / Haplotype-specific extraction (HSE) allows the separation of diploid samples in their haploid components and offers in forensic a new straight forward method to separate Y-chromosomal mixed profiles, consisting of haplotype markers like short tandem repeats (STRs). The advantage of the HSE approach in mixture analysis is the real physical separation of the individual DNA components before the amplification of the STR markers. In order to use the HSE technique for the separation of male DNA mixtures, Y-chromosomal extraction probes were designed to single nucleotide polymorphisms (SNPs), which have been specific for one contributor of the male DNA mixtures. During extraction only complete matched probes are extended by a polymerase which results in the incorporation of biotinylated nucleotides. The synthesized and biotin labeled strand is separated by streptavidin coated magnetic beads. Finally, samples were analyzed by PCR coupled capillary electrophoresis for the detection of the extracted STR markers. First tests of a Y-chromosomal specific extraction showed only little till no enrichment of the targeted alleles. Therefore optimization tests of different parameters were carried out, which revealed the probe design as the key factor of successful HSE. A comparison between simulated probe parameters and their extraction success in HSE showed that the HSE probe efficiency mainly depends of the relation of probe length and GC-contents. Because of the new gained knowledge about the influence of the probe-design on the separation success, probes for future HSE application can be developed faster and cost-effective. The new prediction model for probe-specificity was also successful tested for the extraction of other genome-loci. Furthermore, a multiplex HSE approach was used to separate several STR markers simultaneously in one extraction reaction and therefore achieved the separation of one contributor Y-chromosomal haplotype.

L'analyse de défaillances joue un rôle important dans l'amélioration des performances et de la fabrication des circuits intégrés. Des défaillances peuvent intervenir à tout moment dans la chaîne d'un produit, que ce soit au niveau conception, durant la qualification du produit, lors de la production, ou encore lors de son utilisation. Il est donc important d'étudier ces défauts dans le but d'améliorer la fiabilité des produits. De plus, avec l'augmentation de la densité et de la complexité des puces, il est de plus en plus difficile de localiser les défauts, et ce malgré l'amélioration des techniques d'analyses. Ce travail de thèse s'inscrit dans ce contexte et vise à étudier et développer une nouvelle technique d'analyse de défaillance basée sur l'étude de l'onde laser réfléchie le "Laser Voltage Imaging" (LVI) pour l'analyse de défaillance des technologies ultimes (inférieur à 45nm). / The Failure analysis plays an important role in the improvement of the performances and themanufacturing of integrated circuits. Defects can be present at any time in the product chain,during the conception (design), during the qualification, during the production, or still duringits use. It is important to study these defects in order to improve the reliability of the products.Furthermore, with the density increasing and the complexity of the chips, it is harder andharder to localize the defects. This thesis work consists to develop a new failure analysis technique based on the study of thereflected laser beam the "Laser Voltage Imaging" LVI, for the ultimate technologies (below45nm).