Station siting, exposure, instrumentation, and time of observations influence longterm climatic records. This thesis compared and analyzed temperature data from four Kentucky Mesonet stations located in Fayette (LXGN), Franklin (LSML), Clark (WNCH), and Bullitt (CRMT) counties to two nearby Automated Surface Observation Systems (ASOS) stations in Kentucky. The ASOS stations are located at Louisville International Airport (Standiford Field - KSDF) and at Lexington Airport (Blue Grass Field - KLEX). The null hypothesis states that there is no significant difference in temperature measurements between the two types of stations. To quantify the differences in temperature measurements, geoprofiles and the following statistical procedures were used: coefficient of determination (R2), coefficient of efficiency (E), index of agreement (d), root mean square error (RMSE), and mean absolute error (MAE). Geoprofiles were developed using GIS, and take into account elevation, slope, hillshading, land use, and aspect for each site to help better understand the influence of local topography. It was found that temperature differences could be related to the advancement of weather patterns, vegetation growth and decay, and changes in the landscape at the stations. KSDF consistently recorded higher temperatures than those at CRMT. The positive bias ranged between 0.27 and 2.41 ºC during the time period of September 2009 to August 2010. KLEX was found to be warmer or cooler, with temperature differences that ranged from -1.42 to 0.22 ºC for LXGN, LSML, and WNCH. The index of agreement at KSDF for mean hourly temperatures, when compared to the Bullitt County mesonet station, ranged from 0.88 to 0.99. Meanwhile, the index of agreement at KLEX was 0.96 to 1.00 when compared to the Franklin, Fayette, and Clark mesonet stations. KLEX recorded temperatures that were higher or lower compared to the Franklin, Fayette, and Clark mesonet stations. At the seasonal scale, fall and summer showed larger differences between the Mesonet and ASOS observations. KSDF consistently recorded higher temperatures ranging up to 2.41 °C during the summer. The index of agreement at KSDF in the fall, when compared to the Bullitt County mesonet station average temperatures, ranged from 0.89 to 0.95, while in the summer it was 0.88 to 0.96. The d index indicates a good agreement between ASOS and mesonet stations in winter. KLEX indicates that the index of agreement, RMSE, and MAE are best during winter for all three stations, while in the fall and summer the agreement was not as strong when compared to the Franklin, Fayette, and Clark mesonet stations. In summary, results indicate that the Kentucky Mesonet and ASOS temperature measurements show significant differences throughout the year; therefore, the alternative hypothesis is accepted. These differences are attributed to biases associated with ASOS observations, nearby artificial sources of heating, equipment/maintenance procedures, and land use and land cover at the site.

Mutant mRNA and protein both contribute to the clinical manifestation of many repeat-associated neurodegenerative and neuromuscular disorders. The presence of nuclear RNA clusters is a feature shared amongst these diseases, such as C9ORF72/ALS and myotonic dystrophy 1/2 (DM1/2); however, this pathological hallmark has not been conclusively demonstrated in Huntington’s disease (HD) in vivo. Investigations into HD – caused by a CAG repeat expansion in exon 1 of the huntingtin (HTT) gene – have largely focused on toxic protein gain-of-function as a disease-causing feature, with fewer studies investigating the role of mutant HTT mRNA in pathology or pathogenesis. Here we report that in two HD mouse models, YAC128 and BACHD-97Q-ΔN17, mutant HTT mRNA is preferentially retained in the nucleus in vivo. Furthermore, we observed the early, widespread formation of large mutant HTT mRNA clusters (approximately 0.6 to 5 µm3 in size) present in over 50-75% of striatal and cortical neurons. Affected cells were limited to one cluster at most. Endogenous wild-type mouse Htt or human HTT mRNA containing 31 or fewer repeats did not form clusters. Additionally, the aberrantly spliced N-terminal exon 1-intron 1 RNA fragment, HTT1a, also formed clusters that fully co-localized with the mutant HTT mRNA clusters. These results suggest that multiple repeat-containing transcripts can coalesce to form a single cluster in a given cell. Treating YAC128 mice with antisense oligonucleotides efficiently silenced individual HTT mRNA foci but had limited impact on clusters. Our findings identify mutant HTT mRNA clustering as an early, robust molecular signature of HD, further supporting HD as a repeat expansion disease with suspected mRNA involvement.