"April, 2002" Includes bibliographical references and Publications list (leaves 22-30) V. 1. nos.1-50 -- v. 2. nos. 51-105 "Selected research publications submitted to the University of Adelaide for the degree of Doctor of Science."

"April, 2002" / Includes bibliographical references and Publications list (leaves 22-30) / 2 v. (various paging) : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / "Selected research publications submitted to the University of Adelaide for the degree of Doctor of Science." / Thesis (D.Sc.)--University of Adelaide, Dept. of Environmental Biology, 2003

In this study the enzymatic activity of adsorbed Thermomonospora fusca E��� and Trichoderma reesei CBHI cellulases were investigated using fluorescence techniques. In particular, cellulases were allowed to contact hydrophobic polystyrene surfaces under conditions of different solution concentrations, and adsorption times. Each of these variables is known to have a potential effect on enzyme structure and activity at an interface. Enzymatic activity was measured after partial elution of the adsorbed layer with both protein-free buffer and the surfactant, dodecyltrimethylammonium bromide. For E��� at high concentration (0.5 mg/ml), adsorbed enzyme activity decreased about 20% in increasing adsorption time from 0.25 h to 24 h. At low concentration (0.001 mg/ml), adsorbed enzyme activity decreased by one order of magnitude during a 24 h period. CBHI layers lost activity only after a sufficiently long contact time with the surface, and this effect was not strongly dependent on enzyme concentrations in solution. These findings were explained with reference to structural changes undergone by adsorbed enzyme as a function of time and available interfacial area. / Graduation date: 2000

Graduation date: 1999

Graduation date: 1999

Graduation date: 1998

Graduation date: 1997

No description available.

No description available.

No description available.