Thesis (Ph. D.)--University of Wisconsin--Madison, 1957. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 103-108).

Resumo: As glândulas salivares de carrapatos fêmeas: em jejum, com dois e quatro dias de alimentação (em ingurgitamento), alimentadas (ingurgitadas), com três e sete dias pós-alimentação (pós-ingurgitamento); e de machos: em jejum, com dois, quatro e sete dias de infestação e com três e sete dias pós-remoção do hospedeiro, da espécie Rhipicephalus sanguineus, foram analisadas morfológica, histoquímica e citoquimicamente. Nas fêmeas elas são compostas pelos ácinos I (agranulares), II e III (granulares), e nos machos pelos ácinos I (agranulares), II, III e IV (granulares). Em ambos os sexos também foram observados ácinos Indeterminados, assim chamados devido ao processo degenerativo onde perderam suas características e não puderam ser identificados. Histologicamente os ácinos do tipo I sempre apresentaram uma célula central maior e várias periféricas menores, os do tipo II nas fêmeas apresentaram células "indiferenciadas", indefinidas 1 e 2, a, b e c1 a c6 e nos machos as "indiferenciadas", indefinidas 1 e 2, a, b e c1 a c8, tendo sido as "indiferenciadas", as indefinidas 1 e 2 e as c5 a c8 descritas pela primeira vez. As células aqui denominadas de indeterminadas foram observadas nos ácinos II em estágio de degeneração. Os ácinos do tipo III apresentaram as células d, e e f e os do tipo IV células g. Quanto ao estágio, durante todo o processo de alimentação, tanto nas fêmeas quanto nos machos, os ácinos I sofreram apenas alterações no tamanho. Nos ácinos II de fêmeas em jejum apenas as células "indiferenciadas", indefinidas 1 e 2 e a, c1 e c3 foram observadas; nos ácinos III os três tipos descritos estavam presentes. Nos machos em jejum os ácinos II e III tinham as mesmas características observadas nas fêmeas em jejum. Nos ácinos IV todas as células (g) estavam pouco ativas. Com o iniciar da alimentação, as glândulas passaram... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The salivary glands of the tick Rhipicephalus sanguineus were analyzed morphologically, histochemically and cytochemicallt at the following conditions: unfed, two and four-day fed, engorged females and females at day three and seven post-engorgement; and unfed males and males at day two, four and seven days post-attachment, and at day three and seven post-detachment from the rabbits. In females, these glands consist of types I (agranular), II and III (granular) acini and in males, of types I (agranular), II, III and IV (granular) acini. In both sexes, Indeterminate acini were also observed, which due to the degeneration process, have lost their characteristics and could not be identified. Histologically, type I acinus always exhibited a large central cell and several smaller peripheral ones. In females, type II acini are composed of "undifferentiated", undefined 1 and 2, a, b and c1-c6 ; and in males, "undifferentiated", undefined 1 and 2, a, b and c1-c8, with "undifferentiated", undefined 1 and 2, and c5-c8 being described here for the first time. The cells termed in this study as indeterminate were observed in degenerating type II acini. Type III acinus exhibited cells d, e and f, while type IV acini, cells g. Regarding the feeding stage, throughout the entire process, in females as well as males, type I acinus only underwent changes in size. In type II acinus of unfed females, only "undifferentiated", undefined 1 and 2 and a, c1, and c3 cells were observed, while in type III acinus, the three cell types described were present. In unfed males, type II and III acini exhibited the same characteristics observed in unfed females. In type IV acinus, all cells (g) were little active. With the start of feeding, glands began to secrete actively. In two-day fed females, all cell types were observed in type II acinus, except cells types "undifferentiated", undefined 1 and 2... (Complete abstract, click electronic access below) / Orientador: Maria Izabel Camargo-Mathias / Coorientador: Gervásio Henrique Bechara / Banca: Teresinha Tizu Sato Schumaker / Banca: Patricia Pasquali Parise Maltempi / Banca: Roberta Cornelio Ferreira Nocelli / Banca: Carlos Termignoni / Doutor

Made available in DSpace on 2014-06-11T19:30:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-05-30Bitstream added on 2014-06-13T19:19:40Z : No. of bitstreams: 1 furquim_kcs_dr_rcla.pdf: 3634063 bytes, checksum: c8290792b15c0f58713ff90c2a0aace2 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / As glândulas salivares de carrapatos fêmeas: em jejum, com dois e quatro dias de alimentação (em ingurgitamento), alimentadas (ingurgitadas), com três e sete dias pós-alimentação (pós-ingurgitamento); e de machos: em jejum, com dois, quatro e sete dias de infestação e com três e sete dias pós-remoção do hospedeiro, da espécie Rhipicephalus sanguineus, foram analisadas morfológica, histoquímica e citoquimicamente. Nas fêmeas elas são compostas pelos ácinos I (agranulares), II e III (granulares), e nos machos pelos ácinos I (agranulares), II, III e IV (granulares). Em ambos os sexos também foram observados ácinos Indeterminados, assim chamados devido ao processo degenerativo onde perderam suas características e não puderam ser identificados. Histologicamente os ácinos do tipo I sempre apresentaram uma célula central maior e várias periféricas menores, os do tipo II nas fêmeas apresentaram células indiferenciadas, indefinidas 1 e 2, a, b e c1 a c6 e nos machos as indiferenciadas, indefinidas 1 e 2, a, b e c1 a c8, tendo sido as indiferenciadas, as indefinidas 1 e 2 e as c5 a c8 descritas pela primeira vez. As células aqui denominadas de indeterminadas foram observadas nos ácinos II em estágio de degeneração. Os ácinos do tipo III apresentaram as células d, e e f e os do tipo IV células g. Quanto ao estágio, durante todo o processo de alimentação, tanto nas fêmeas quanto nos machos, os ácinos I sofreram apenas alterações no tamanho. Nos ácinos II de fêmeas em jejum apenas as células indiferenciadas, indefinidas 1 e 2 e a, c1 e c3 foram observadas; nos ácinos III os três tipos descritos estavam presentes. Nos machos em jejum os ácinos II e III tinham as mesmas características observadas nas fêmeas em jejum. Nos ácinos IV todas as células (g) estavam pouco ativas. Com o iniciar da alimentação, as glândulas passaram... / The salivary glands of the tick Rhipicephalus sanguineus were analyzed morphologically, histochemically and cytochemicallt at the following conditions: unfed, two and four-day fed, engorged females and females at day three and seven post-engorgement; and unfed males and males at day two, four and seven days post-attachment, and at day three and seven post-detachment from the rabbits. In females, these glands consist of types I (agranular), II and III (granular) acini and in males, of types I (agranular), II, III and IV (granular) acini. In both sexes, Indeterminate acini were also observed, which due to the degeneration process, have lost their characteristics and could not be identified. Histologically, type I acinus always exhibited a large central cell and several smaller peripheral ones. In females, type II acini are composed of undifferentiated, undefined 1 and 2, a, b and c1-c6 ; and in males, undifferentiated, undefined 1 and 2, a, b and c1-c8, with undifferentiated, undefined 1 and 2, and c5-c8 being described here for the first time. The cells termed in this study as indeterminate were observed in degenerating type II acini. Type III acinus exhibited cells d, e and f, while type IV acini, cells g. Regarding the feeding stage, throughout the entire process, in females as well as males, type I acinus only underwent changes in size. In type II acinus of unfed females, only undifferentiated, undefined 1 and 2 and a, c1, and c3 cells were observed, while in type III acinus, the three cell types described were present. In unfed males, type II and III acini exhibited the same characteristics observed in unfed females. In type IV acinus, all cells (g) were little active. With the start of feeding, glands began to secrete actively. In two-day fed females, all cell types were observed in type II acinus, except cells types undifferentiated, undefined 1 and 2... (Complete abstract, click electronic access below)

Storage mite Acarus siro is one of the most distributed stored product mites in the world. It infests various products (grains, dried fruits, meat products, animal feed etc.) and causes allergic reactions in humans. For these reasons, it is important to find an effective strategy to suppress or even better to eliminate the mite from the storing facilities. Historically, there have been reported cases of the mite being resistant to several pesticides. In this thesis, four populations of Acarus siro were exposed to pesticides in different concentrations - first in the form of solutions and next as a diet additive. The populations showed divergent sensitivity to four selected pesticides (pirimiphos-methyl, chlorpyriphos-methyl, deltamethrin and deltamethrin in combination with piperonyl butoxide). The biggest differences were recorded in response to solutions of pirimiphos-methyl. This pesticide was then added to standard rearing diet in five concentrations. The mite populations were exposed to this died for 3 weeks. Control and pesticide-treated diet microbiome analyses revealed that 0.0125 µg×g-1 concentration causes hormoligosis in 6L and 6Tu strains. The same concentration was responsible for microbiome change in 6Z strain. Exposure to 1,25 µg×g-1 concentration caused microbial shifts in 6Z and...