The treatment of acid mine drainage (AMD) by sulfate-reducing bacteria (SRB) has been reported in the literature as a possible alternative to chemical treatment. The overall objective of this study was to implement such a treatment process at the mine site and specifically within the open mine pit itself. The first step of this study was to characterize process parameters. To accomplish this, a reactor was designed and built to simulate hydrodynamic conditions found in the mine pit. This reactor contained a 6$ sp{ prime prime}$ deep gravel bed in which a mixed population of SRB was inoculated. The operation of this reactor demonstrated that treatment of a continuous flow of AMD by SRB was possible, however, the response to changes in the composition of the AMD and to flow conditions was limited. The results indicated that further studies should be directed at the gravel bed itself since this was where the SRB are located and is the active site within the system. / The role of various physical parameters of a gravel bed in the biological treatment of AMD by SRB was examined. This was accomplished by using gravel of different sizes (0.25$ sp{ prime prime}$ vs. 0.5$ sp{ prime prime})$ and composition (granite vs limestone) to form 12$ sp{ prime prime}$ beds in a series of column reactors. The difference in size results in variations in the total surface area, the void volume and various volume ratios within the system. The effect of potential geological buffering by limestone was examined by using and comparing with beds composed of granite. / The gravel beds were inoculated with a mixed culture of SRB and overlaid with 6 L of AMD. After the SRB were established, a series of experiments were performed in which 16.7%, 25%, 75% and 100% of the water column was replaced with an equivalent quantity of fresh AMD. Changes in pH, ORP, electric conductivity, and concentrations of metal and sulfate were monitored for at least 28 days. Sulfate and metal removal at days 7 and 28 of each experiment were compared. The SRB can tolerate a wide range of disturbances, however, an increase in the load of fresh AMD decreased the performance of the system. The results indicated that the total surface area is of greater importance than the void volume in the overall treatment process by SRB. / A dimensionless number was constructed to describe the relationships between the physical parameters of the gravel bed. A plot of the proportion of sulfate removed and this dimensionless number could provide essential information for the sizing of a gravel bed for the purposes of sulfate reduction. This was done for each type of gravel and comparison of the two curves indicated that there was no significant difference between the two gravels. / The importance of the physical substrate for the SRB has been reported in the literature. However, no known attempt has been made to quantify the relationships between the physical parameters and the biological activity. Such information would be useful for the sizing of wetlands and other passive treatment system that uses SRB activity for the purposes of treating AMD. This study is a step towards filling this void.

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