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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

The location, expression and regulation of three antibiotic resistance genes in acinetobacter

Elisha, Brenda Gay 21 September 2023 (has links) (PDF)
Clinical isolates of Acinetobacter often exhibit resistance to many antibiotics, including the aminoglycosides and chloramphenicol. Yet very little is known about this resistance at the molecular level. A strain of Acinetobacter baumannii (strain SAK) was isolated from a tracheal aspirate; it is resistant to gentamicin, tobramycin, netilmicin, streptomycin, chloramphenicol and suphamethoxazole. This study examines the nature of the resistance to gentamicin, tobramycin and chloramphenicol at three levels: the biochemical mechanism of resistance, the location of the resistance genes, and the expression and regulation of these genes. Two aminoglycoside resistance genes, and a chloramphenicol resistance gene have been identified in strain SAK. The aminoglycoside resistance genes, aadB and aacC, encode AAD (2") and AAC(3), respectively. Both of the enzymes have activity against gentamicin and tobramycin. DNA: DNA hybridization with specific DNA probes indicates that the aadB gene is in the chromosome, whereas the aacC and cat genes have a plasmid and a chromosomal locus. Each of these genes was cloned and expressed in E. coli. Interestingly, although the aadB gene is functional ·in E.coli, RNA studies indicate that it is not expressed in strain SAK. Only aacC gene transcripts were detected in this strain. Thus, strain SAK contains a seemingly redundant aadB gene. DNA sequencing data show that the aadB gene is part of a Tn2J-like transposon; similarly, the cat gene is also part of a transposon which may be identical to Tn2670. In a limited study, it was not possible to activate the aadB gene in strain SAK. There is DNA sequencing evidence to suggest that the aacC gene may be linked to an IS, and that it may have a catabolite sensitive promoter. Papers based on the work described in this dissertation have been accepted for publication. in: Plasmid (Elisha,B.G. & Steyn,L.M., 1991); Current Microbiology (Elisha,B.G. & Steyn,L.M., 1991); FEMS Symposium Series: The Biology of Acinetobacter. K.J.Towner & C.A.Fewson (eds.). Plenum Press. New York. (Elisha,B.G. & Steyn,L.M., 1991).
2

Serological and DNA characterization of Acinetobacter phosphadevorus

Kronland, Wallace Carl January 1981 (has links)
No description available.
3

Characterization of the Specificity of DNA Uptake and Transformation by Naturally Competent Acinetobacter Calcoaceticus

Ahmadian-Tehrani, Mohsen 08 1900 (has links)
Although cultures of A. calcoaceticus BD413 are naturally competent at all growth phases, genetic markers can only be successfully transformed by DNA from the genus Acinetobacter. Transformation frequencies of nearly 1.2% were obtained with pcaA mutants were incubated in liquid culture with wild-type DNA.
4

A genetic study of Acinetobacter phosphadevorus

Garvey, Kevin James January 1981 (has links)
No description available.
5

Metabolism of lower chain carbon compounds by Acinetobacter phosphadevorus

Jones, Ronald Dale January 1981 (has links)
No description available.
6

Acinetobacter phosphadeuorus: phosphate uptake and initial studies of metabolism

Kutzer, Ralph Wesley, 1949- January 1975 (has links)
No description available.
7

Characterization and metabolic studies of Acinetobacter phosphadevorus

Ferguson, Tommy Leon, 1951- January 1976 (has links)
No description available.
8

Analyse der Interaktion von Acinetobacter baumannii mit humanen epithelialen Zellen

Lübeck, Anke, January 2008 (has links)
Ulm, Univ., Diss., 2008.
9

Transformation und Pilusbiogenese zwei voneinander unabhängige Systeme in Acinetobacter sp. BD413 /

Gohl, Olivia. January 1900 (has links) (PDF)
Göttingen, Univ., Diss., 2002. / Computerdatei im Fernzugriff.
10

Transformation und Pilusbiogenese zwei voneinander unabhängige Systeme in Acinetobacter sp. BD413 /

Gohl, Olivia. January 1900 (has links) (PDF)
Göttingen, Universiẗat, Diss., 2002.

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