This thesis consists of two parts: a report on the use of a binocular Shack-Hartmann (SH) sensor to study the dynamic correlation of ocular aberrations; and the application of an adaptive optics (AO) system to investigate the effect of the manipulation of aberrations on the accommodation control. The binocular SH sensor consists of one laser source and one camera to reduce system cost and complexity. Six participants took part in this study. Coherence function analysis showed that coherence values were dependent on the subject, aberration and frequency component. Inter-ocular correlations of the aberration dynamics were fairly weak for all participants. Binocular and monocular viewing conditions produced similar wavefront error dynamics. The AO system has a dual wavefront sensing channel. The extra sensing channel permits direct measurement of the eye's aberrations independent of the deformable mirror. Dynamic correction of aberrations during steady-state fixation did not affect the accommodation microfluctuations, possibly due to the prior correction of the static aberration level and/or the limited correction bandwidth. The inversion of certain aberrations during dynamic accommodation affected the gain and latency of accommodation response (AR), suggesting that the eye used the aberrations to guide its initial path of accommodative step response. Corrections of aberrations at various temporal locations of AR cycle produced subject- and aberration-dependent results. The gain and phase lag of the AR to a sinusoidally moving target were unaffected by aberration correction. The predictable nature of the target had been suggested as the reason for its failure to produce any significant effect on the AR gain and phase lag.

This thesis consists of two parts: a report on the use of a binocular Shack-Hartmann (SH) sensor to study the dynamic correlation of ocular aberrations; and the application of an adaptive optics (AO) system to investigate the effect of the manipulation of aberrations on the accommodation control. The binocular SH sensor consists of one laser source and one camera to reduce system cost and complexity. Six participants took part in this study. Coherence function analysis showed that coherence values were dependent on the subject, aberration and frequency component. Inter-ocular correlations of the aberration dynamics were fairly weak for all participants. Binocular and monocular viewing conditions produced similar wavefront error dynamics. The AO system has a dual wavefront sensing channel. The extra sensing channel permits direct measurement of the eye¿s aberrations independent of the deformable mirror. Dynamic correction of aberrations during steady-state fixation did not affect the accommodation microfluctuations, possibly due to the prior correction of the static aberration level and/or the limited correction bandwidth. The inversion of certain aberrations during dynamic accommodation affected the gain and latency of accommodation response (AR), suggesting that the eye used the aberrations to guide its initial path of accommodative step response. Corrections of aberrations at various temporal locations of AR cycle produced subject- and aberration-dependent results. The gain and phase lag of the AR to a sinusoidally moving target were unaffected by aberration correction. The predictable nature of the target had been suggested as the reason for its failure to produce any significant effect on the AR gain and phase lag.

Studies demonstrate neuro-retinal damage in patients with diabetes and no clinically visible diabetic retinopathy. It is unknown which retinal regions are most vulnerable to diabetes. We hypothesized that the standard and slow-flash (sf-) multifocal electroretinogram (mfERG) and adaptive optics (AO) imaging will localize retinal regions of vulnerability. Fifty-five adolescents with diabetes and 54 controls underwent mfERG testing to isolate predominately retinal bipolar cell activity and sf-mfERG testing to isolate three oscillatory potentials (OPs) from intraretinal amacrine and interplexiform cells. Greatest mfERG delays were in the superior temporal quadrant and at 5°-10° eccentricity. Greatest sf-mfERG delays were found at different eccentricities for each OP. Twenty adolescents with diabetes and 14 controls underwent AO imaging. No significant differences in cone photoreceptor density were found; however, patients showed a trend towards reduced density in the superior nasal region. Inner retinal structures may be more susceptible to damage by diabetes than outer retinal structures.

Studies demonstrate neuro-retinal damage in patients with diabetes and no clinically visible diabetic retinopathy. It is unknown which retinal regions are most vulnerable to diabetes. We hypothesized that the standard and slow-flash (sf-) multifocal electroretinogram (mfERG) and adaptive optics (AO) imaging will localize retinal regions of vulnerability. Fifty-five adolescents with diabetes and 54 controls underwent mfERG testing to isolate predominately retinal bipolar cell activity and sf-mfERG testing to isolate three oscillatory potentials (OPs) from intraretinal amacrine and interplexiform cells. Greatest mfERG delays were in the superior temporal quadrant and at 5°-10° eccentricity. Greatest sf-mfERG delays were found at different eccentricities for each OP. Twenty adolescents with diabetes and 14 controls underwent AO imaging. No significant differences in cone photoreceptor density were found; however, patients showed a trend towards reduced density in the superior nasal region. Inner retinal structures may be more susceptible to damage by diabetes than outer retinal structures.