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The Role of ShcA Phosphotyrosine Signaling in the MyocardiumVanderlaa, Rachel 31 August 2011 (has links)
Tyrosine kinases (TK) are important for cardiac function, but their downstream targets in the adult heart have yet to be established. The ShcA docking protein binds specific phosphotyrosine (pTyr) sites on activated TKs through its N-terminal PTB and C-terminal SH2 domains and stimulates downstream pathways through motifs such as pTyr sites in its central CH1 region. To explore the role of this TK scaffold in the adult heart, we generated a myocardial-specific knockout of murine ShcA (ShcA CKO). Such mice developed a dilated cardiomyopathy phenotype involving impaired systolic function with enhanced cardiomyocyte contractility. This uncoupling of global heart and intrinsic myocyte functions was associated with altered perimysial collagen and extracellular matrix complicance properties, suggesting disruption of mechanical coupling. In vivo dissection of ShcA signaling properties revealed that selective inactivation of the PTB domain in the myocardium had effects resembling those seen in ShcA CKO mice, while disruption of the SH2 domain caused a less severe cardiac phenotype. Downstream signaling through the CH1 pTyr sites was dispensable for baseline cardiac function, but necessary to prevent adverse remodeling after hemodynamic overload. Therefore, ShcA mediates pTyr signaling in the adult heart through multiple distinct signaling elements that control myocardial functions and response to stresses.
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The Role of ShcA Phosphotyrosine Signaling in the MyocardiumVanderlaa, Rachel 31 August 2011 (has links)
Tyrosine kinases (TK) are important for cardiac function, but their downstream targets in the adult heart have yet to be established. The ShcA docking protein binds specific phosphotyrosine (pTyr) sites on activated TKs through its N-terminal PTB and C-terminal SH2 domains and stimulates downstream pathways through motifs such as pTyr sites in its central CH1 region. To explore the role of this TK scaffold in the adult heart, we generated a myocardial-specific knockout of murine ShcA (ShcA CKO). Such mice developed a dilated cardiomyopathy phenotype involving impaired systolic function with enhanced cardiomyocyte contractility. This uncoupling of global heart and intrinsic myocyte functions was associated with altered perimysial collagen and extracellular matrix complicance properties, suggesting disruption of mechanical coupling. In vivo dissection of ShcA signaling properties revealed that selective inactivation of the PTB domain in the myocardium had effects resembling those seen in ShcA CKO mice, while disruption of the SH2 domain caused a less severe cardiac phenotype. Downstream signaling through the CH1 pTyr sites was dispensable for baseline cardiac function, but necessary to prevent adverse remodeling after hemodynamic overload. Therefore, ShcA mediates pTyr signaling in the adult heart through multiple distinct signaling elements that control myocardial functions and response to stresses.
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The SH2 Domain-Containing Adaptor Protein SHD Reversibly Binds the CRKL-SH2 Domain and Knockdown of shdb Impairs Zebrafish Eye DevelopmentChandler, Brendan 01 January 2018 (has links)
The adaptor protein CT10-Regulator of Kinase (CRK) and the closely related CRK-Like (CRKL) are adaptor proteins that play important roles in many signaling pathways regulating cell proliferation and cell motility. A notable example is their required role in Reelin signaling during development of the laminated structures of the vertebrate central nervous system, including the cerebral cortex, cerebellum, hippocampus, and retina. As adaptors, CRK/CRKL are important in coupling phosphotyrosine signaling to G protein activity to regulate both cell proliferation and changes in the actin cytoskeleton, thereby exerting control over cell motility, and migration. While many proteins that interact with CRK/CRKL have been identified, the diverse roles of these molecules suggest that more remain to be found.
Herein is described a novel CRK/CRKL interacting protein, Src Homology 2 domain-containing protein D (SHD), which demonstrates a phosphorylation-dependent interaction with the CRK/CRKL SH2 domain in HEK 293 cells stimulated with hydrogen peroxide, which globally boosts tyrosine phosphorylation by inhibiting tyrosine phosphatases. Treatment with an inhibitor for Src family kinases (SFKs), Src-1, or an inhibitor of Abl/Arg kinases, STI571, reduces peroxide-induced binding of the CRKL-SH2 domain to SHD. We show that overexpression of Abl kinase, but not the SFK Fyn is sufficient to induce binding of the CRKL-SH2 to SHD and that this interaction requires at least one of the five tyrosines in YxxP motifs found in SHD. Using mass spectrometry, we found that Abl phosphorylates SHD on Y144, which is located in a YxxP motif. Mutation of this site to phenylalanine reduces, but does not prevent, Abl-induced binding of SHD to the CRKL-SH2 domain, suggesting that other YxxP sites also facilitate the interaction. A discussion of the cellular consequences of the interaction between SHD and CRK/CRKL is presented.
To explore the biological role of SHD, we used the zebrafish to study shdb, a putative ortholog of human SHD. The expression of shdb was unknown and so we performed in situ hybridization and determined that shdb was expressed in the developing nervous system. To study the function of this gene, we used a morpholino to knock down expression of shdb which resulted in significantly reduced eye size. Possible roles of Shdb in eye development are discussed as is future research aimed to elucidate the cellular and developmental mechanisms by which Shdb functions in the developing eye.
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The role of APPL1 in the adiponectin signaling : a dissertation /Mao, Xuming. January 2006 (has links)
Dissertation (Ph.D.).--University of Texas Graduate School of Biomedical Sciences at San Antonio, 2006. / Vita. Includes bibliographical references.
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Neuronal insulin signaling and the regulation of mammalian lifespan a dissertation /Ramos, Fresnida. January 2008 (has links)
Dissertation (Ph.D.).--University of Texas Graduate School of Biomedical Sciences at San Antonio, 2008. / Vita. Includes bibliographical references.
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The role of sequestosome 1 (SQSTM1) in Paget's disease of bone a dissertation /Rhodes, Emily C. January 2008 (has links)
Dissertation (Ph.D.) --University of Texas Graduate School of Biomedical Sciences at San Antonio, 2008. / Vita. Includes bibliographical references.
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Activity-Regulated Cytoskeleton-Associated Protein Controls AMPAR Endocytosis through a Direct Interaction with Clathrin-Adaptor Protein 2DaSilva, L.L., Wall, M.J., de Almeida, Luciana P., Wauters, S.C., Januario, Y.C., Muller, Jurgen, Corrêa, Sonia A.L. 18 April 2016 (has links)
Yes / The activity-regulated cytoskeleton-associated (Arc) protein control synaptic strength by facilitating AMPA receptor (AMPAR) endocytosis. Here we demonstrate that Arc targets AMPAR to be internalized through a direct interaction with the clathrin-adaptor protein 2 (AP-2). We show that Arc overexpression overexpression in dissociated hippocampal neurons obtained from C57BL/6 mouse reduces the density of AMPAR GluA1 subunits at the cell surface and reduces the amplitude and rectification of AMPAR-mediated miniature-excitatory postsynaptic currents (mEPSC). Mutations of Arc, that prevent the AP-2 interaction reduce Arc-mediated endocytosis of GluA1 and abolish the reduction in AMPAR-mediated mEPSC amplitude and rectification. Depletion of the AP-2 subunit µ2 blocks the Arc-mediated reduction in mEPSC amplitude, effect that is restored by re-introducing µ2. The Arc/AP-2 interaction plays an important role in homeostatic synaptic scaling as the Arc-dependent decrease in mEPSC amplitude, induced by a chronic increase in neuronal activity, is inhibited by AP-2 depletion. This data provides a mechanism to explain how activity-dependent expression of Arc decisively controls the fate of AMPAR at the cell surface and modulates synaptic strength, via the direct interaction with the endocytic clathrin adaptor AP-2. / This work was supported by the BBSRC_FAPPA BB/J02127X/1 and BBSRC-BB/H018344/1 to SALC and by the FAPESP_RCUK_FAPPA 2012/50147-5 and FAPESP_Young Investigator’s grant 2009/50650-6 to LLdS. SCW was a PhD Student supported be the BBSRC/GSK PhD-CASE Studentship, LPdA is a postdoc fellow supported by FAPESP, YCJ was supported by a FAPESP scientific initiation scholarship.
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Sorting nexin 9 in clathrin-mediated endocytosis /Lundmark, Richard, January 2004 (has links)
Diss. (sammanfattning) Umeå : Univ., 2004. / Härtill 3 uppsatser.
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Structural and biochemical studies of cell death signaling /Park, Hyun Ho. January 2007 (has links)
Thesis (Ph. D.)--Cornell University, August, 2007. / Vita. Includes bibliographical references (leaves 196-214).
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B-cell-antigen receptor endocytosis uses a distinct signaling pathway, involving LAB, Vav, dynamin and Grb2Malhotra, Shikha. January 2009 (has links) (PDF)
Thesis (Ph. D.)--University of Oklahoma. / Bibliography: leaves 155-195.
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