Made available in DSpace on 2014-12-17T15:32:33Z (GMT). No. of bitstreams: 1 MarciaCCMiguel_tese.pdf: 1534160 bytes, checksum: 1a3ec415bfaf10c088ec1ae5f6224189 (MD5) Previous issue date: 2005-05-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Pleomorphic adenoma and adenoid cystic carcinoma (ACC) consist benign and malignant neoplasm from salivary gland, respectively. These neoplasms share some characteristics, such as cellular origin and considerable production of extracellular matrix, however, with distinct biological behavior. The aim of the present study was to compare the expression of D2E1, D3E1 e D5E1 integrins in pleomorphic adenoma from minor and major salivary glands and ACCs. Furthermore, it was investigated possible differences in the expression of these integrins according to histological subtypes of ACC. Fourteen cases of pleomorphic adenoma from major salivary gland, fourteen cases from minor salivary gland and ten cases of ACC were selected. It was taken into consideration the presence or absence, localization and intensity of integrin immunoexpression. The cases of pleomorphic adenoma were grouped in order to compare the expression between the distinct neoplasms. It was observed a highly significant difference (p<0,0001) in relation to D2E1 integrin between the neoplasms since pleomorphic adenoma showed a pronounced immunostaining. It was not possible to perform statistical tests considering the D2E1 integrin expression; nevertheless, it could be observed a tendency of higher staining in pleomorphic adenoma. For comparative reasons the cases ACCs were divided in two groups: solid and tubular/cribriform. It was not detected significant differences in regard to D2E1 integrin; and statistical analysis could not be realized in relation to D3E1 and D5E integrin expression. However, it was also verified a tendency of absence or reduced expression in the solid subtype. It can be concluded that the reduced D2E1 integrin expression observed in CACs may be related to a lesser degree of cell differentiation in this neoplasm and the reduced D5E1 integrin expression can be associated with aggressive biological behavior. Moreover, the absence and/or reduced expression of the studied integrins in solid ACC suggests a role in pathogenesis and more aggressive biological behavior of this histological subtype / O adenoma pleom?rfico e o carcinoma aden?ide c?stico (CAC) representam neoplasias de gl?ndula salivar benigna e maligna, respectivamente, as quais compartilham algumas caracter?sticas como a mesma origem celular e uma marcante presen?a de matriz extracelular, apresentando, por?m, comportamentos biol?gicos distintos. O prop?sito desta pesquisa consistiu em comparar a express?o das integrinas D2E1, D3E1 e D5E1 em adenomas pleom?rficos de gl?ndula salivar menor e maior e CACs. Al?m disso, procurou investigar se havia diferen?as na express?o destas integrinas entre os subtipos histopatol?gicos do CAC. Foram selecionados 14 casos de adenoma pleom?rfico de gl?ndula salivar maior, 14 casos de gl?ndula salivar menor e 10 casos de CACs. Analisou-se a presen?a ou aus?ncia, localiza??o e intensidade de marca??o das integrinas. Os dois grupos de adenomas pleom?rficos foram reunidos em um s? para fazer a compara??o entre os dois tumores. Verificou-se que houve diferen?a estat?stica altamente significativa (p<0,0001) para a integrina D2E1 entre os dois tumores, apresentando o adenoma pleom?rfico, uma marca??o mais intensa para esta integrina. Em rela??o ? integrina D5E1 n?o foi poss?vel a realiza??o de testes estat?sticos, ficando patente, por?m, que houve uma tend?ncia da referida integrina ser mais intensamente expressa no adenoma pleom?rfico. Para an?lise comparativa, os CACs foram subdivididos em 2 grupos: s?lido e tubular/cribriforme. Para a integrina D2E1 observou-se que n?o houve diferen?a estatisticamente significativa e em rela??o ? D3E1 e D5E1 n?o foi poss?vel a realiza??o do teste estat?stico; no entanto, tamb?m foi verificada uma clara tend?ncia para os casos do subtipo s?lido apresentarem express?o ausente ou reduzida das integrinas avaliadas. Concluiu-se que a reduzida express?o da integrina D2E1 observada nos CACs, pode estar relacionada com a menor diferencia??o das c?lulas deste tumor e ? poss?vel que a reduzida express?o da D5E1, possa estar implicada em seu comportamento mais agressivo. Al?m disso, sugere-se que a aus?ncia e/ou redu??o da express?o das integrinas pesquisadas nos casos do subtipo s?lido, pode desempenhar algum papel na patog?nese e no comportamento biol?gico mais agressivo deste subtipo tumoral

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Made available in DSpace on 2014-12-17T15:32:22Z (GMT). No. of bitstreams: 1 LucileideCO_DISSERT.pdf: 2248988 bytes, checksum: f9aafad24354c13fb349a052f5b90d8e (MD5) Previous issue date: 2012-06-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The expression of glucose transporter protein 1 (GLUT-1), as well the angiogenesis has been associated to clinical behavior and aggressiveness in tumors of various origin. It is believed that the expression of this protein denotes metabolic demand of the tumor cells and, thus its influence upon the formation of new blood vessels. Pleomorphic adenoma (PA) and the adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (MEC) represent, respectively, the most commom benign and malignant tumors of salivary glands. The aim of this study was to analyze and compare the immunohistochemical expression of GLUT-1 and its correlation with angiogenesis in cases of PAs, ACCs and MECs considering their histological grades. The sample consisted of 20 PAs, 20 ACCs and 10 MECs. The cases were analyzed and classified according to their histological grades. The expression of GLUT-1 was evaluated in the parenchyma lesions, establishing the percentage of immunopositive cells, according to the following scores: 0 (no cell immunomarked), 1 (up to 25% of tumor cells immunostained), 2 (25 - 50% of tumor cells immunostained) and 3 (more than 50% of tumor cells immunostained). The angiogenic index was analyzed by counting the microvessels immunostained by anti-CD34 antibody, in 5 fields (200X). The analysis of the expression of GLUT-1 in tumor parenchyma showed statistically significant differences between benign and malignant groups (p = 0.022). The average number of microvessels in PAs was 40.4, 21.2 in ACCs and 66.5 in MECs, with significant differences between groups (p <0.001). When compared to the expression of GLUT-1 and angiogenic index as a whole, there was no significant correlation between the number of microvessels and the expression of GLUT-1 (r = 0.211, p = 0.141). In conclusion, the results of this study suggest not only that differences in biological behavior between PAs, ACCs and MECs may be associated to the expression of GLUT-1, but also that benign and malignant salivary gland present differences in the average number of microvessels, with higher levels considered more aggressive tumors. Furthermore, the number of newly formed microvessels can be independent of the metabolic demand of the tumor cells / A express?o da prote?na transportadora de glicose tipo 1 (GLUT-1), bem como a angiog?nese, t?m sido relacionadas ao comportamento cl?nico e agressividade em neoplasias de origem diversas. Acredita-se que a express?o desta prote?na denote a demanda metab?lica das c?lulas tumorais e, assim, a sua influ?ncia na forma??o de novos vasos sanguineos. O adenoma pleom?rfico (AP) e o carcinoma adenoide c?stico (CAC) e carcinoma mucoepiderm?ide (CME) representam, respectivamente, a neoplasia benigna e as malignas mais frequentes das gl?ndulas salivares. O prop?sito deste estudo foi comparar a express?o imuno-histoqu?mica da GLUT-1, bem como correlacionar com a angiog?nese em casos de APs, CACs e CMEs levando em considera??o suas grada??es histol?gicas. A amostra foi composta por 20 APs, 20 CACs e 10 CMEs os quais foram classificados de acordo com os graus histol?gicos apresentados. A express?o da GLUT-1 foi avaliada no par?nquima das les?es, estabelecendo-se o percentual de c?lulas imunopositivas, de acordo com os escores: 0 (nenhuma c?lula imunomarcada), 1 (at? 25% das c?lulas tumorais imunomarcadas), 2 (de 25-50% das c?lulas tumorais imunomarcadas) e 3 (mais de 50% das c?lulas tumorais imunomarcadas). O ?ndice angiog?nico foi analisado por meio da contagem de microvasos imunomarcados pelo anticorpo anti-CD34, em 5 campos (200x). A an?lise da express?o da GLUT-1 revelou diferen?as estatisticamente significativas entre os grupos benignos e malignos (p = 0,022). O n?mero m?dio de microvasos foi de 40,4 em APs, 21,2 em CACs e 66,5 em CMEs, com diferen?as significativas entre os grupos (p < 0,001). Quando comparadas a express?o da GLUT-1 com o ?ndice angiog?nico em conjunto, n?o foi evidenciada correla??o significativa entre a quantidade de microvasos e a express?o da GLUT-1 (r = 0,211; p = 0,141). Os resultados do presente estudo sugerem que as diferen?as no comportamento biol?gico entre APs, CACs e CMEs podem estar relacionadas ? express?o da GLUT-1 e que tumores benignos e malignos de gl?ndulas salivares exibem diferen?as no n?mero m?dio de microvasos, com maiores ?ndices nos tumores considerados mais agressivos. Al?m disto, o n?mero de microvasos neoformados pode ser independente da demanda metab?lica das c?lulas tumorais