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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Photoaffinity labeling of Nak-ATPase with [¹²⁵I]-iodoazidocymarin

Lowndes, Joseph M. January 1983 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1983. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 86-87).
2

Photoaffinity labelling of the ouabain binding site of sodium-potassium-activated adenosine triphosphatase

Hall, Clifford Charles. January 1981 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1981. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 288-296).
3

Purification, reconstitution, and characterization of the ATP synthetase of Escherichia coli

Foster, David L. January 1980 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1980. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
4

Purification of the sodium-potassium transport adenosinetriphosphatase

Dulak, Norman Charles, January 1970 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1970. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.
5

Characterization of the vacuolar H r-AtPase of higher plants

Manolson, Morris F. January 1988 (has links)
No description available.
6

VCP/p97 is required for the timely degradation of p27 in G0/G1 to S phase transition in MCF-7 cell

Shi, Xianli, 石现丽 January 2014 (has links)
VCP/p97 works as a segregase to extract the ubiquitylated proteins from protein complexes, lipid membranes and chromosomes, thereby promoting their degradation or recycling. VCP/p97 plays essential roles in ubiquitin-dependent proteasome degradation, ERAD, autophagy, endocytosis, reassembly of ER, Golgi and nuclear envelop, and cell cycle regulation. In ubiquitin dependent proteasome degradation pathway, VCP/p97, as a special ubiquitin binding-shuttle factor, is required for the successful cell cycle progression in regulating IκB, CDT-1, Aurora B, and CDC25A. Here, we studied the role of VCP/p97 in G1 to S phase transition in MCF-7 human breast cancer cells. We found that VCP/p97 knockdown or inhibition by DBeQ, a potent VCP/p97 inhibitor, decreased cell proliferating rates and reduced S phase cell percentages in asynchronized MCF-7 cells.VCP/p97 inhibition by DBeQ also arrested cells at G1 phase in synchronized MCF-7 cells. These data suggest that VCP/p97 is required for G0/G1 to S phase transition in MCF-7 cells. In addition, in either asynchronized or synchronized MCF-7 cells, VCP/p97 knockdown or DBeQ treatment resulted in the accumulation of p21 and p27, two CDK inhibitors. Moreover, p27, not p21, knockdown in MCF-7 cells rescued the defects of S phase entry caused by VCP/p97 knockdown or DBeQ treatment. Taken together, our results suggest that VCP/p97 regulates the timely degradation of p27 to promote G1 to S phase transition in MCF-7 cells. / published_or_final_version / Physiology / Master / Master of Philosophy
7

Characterization of the vacuolar H r-AtPase of higher plants

Manolson, Morris F. January 1988 (has links)
The tonoplast H$ sp+$-ATPase of Beta vulgaris L. was partially purified by Triton X-100 solubilization and Sepharose 4B chromatography resulting in the enrichment of two polypeptides (57 and 67 kDa). Kinetic analysis of ($ alpha$-$ sp{32}$P) BzATP labeling identified the 57 kDa polypeptide as a nucleotide-binding subunit with a possible regulatory function. In addition, ($ sp{14}$C) DCCD-labeling identified a 16 kDa polypeptide as a putative transmembrane proton channel. It is concluded that the tonoplast H$ sp+$-ATPase is a multimer composed of at least three polypeptides. / Anti-57 and anti-67 kDa sera reacted with polypeptides of the corresponding size in bovine chromaffin granules, bovine clathrin-coated vesicles, and yeast vacuolar membranes, suggesting common structural features and common ancestry for endomembrane H$ sp+$-ATPases of different organelles and different phyla. Anti-57 serum was used to isolate a cDNA encoding the corresponding subunit from Arabidopsis. Protein sequence analysis revealed homologies between endomembrane, F$ sb0$F$ sb1$ and archaebacterial ATPases, suggesting that these different classes of ATPases have evolved from a common ancestor.
8

Cytochemical localization of adenosine triphosphatase in the nuclear envelope of necturus maculosus oocytes

Bergdall, Kristin Miller January 1988 (has links)
Cytochemical localization of nuclear-envelope nucleos-side triphosphatase was exhibited in the nuclear membrane and nuclear pores of the Necturus, maculosus oocyte. This enzyme is thought to promote nucleocytoplasmic transport of mRNA through the nuclear pores. Various tissue preparations were performed to assure optimum results of reaction product formation and preservation of tissue ultrastructure.Incubation of frozen oocyte sections in a modified Wachstein-Meisel medium resulted in positive staining of the nuclear membrane and the nucleoli as evidenced in light and electron micrographs. Whole oocytes were incubated in the Wachstein-Meisel medium and then embedded in Epon for electron microscopy. The whole oocytes contained reaction product associated with microvilli and plasma membranes. Exposure of manually isolated nuclei to the same experimental medium resulted in lead deposits in the nuclear envelope, the nuclear pores, and randomly dispersed among chromatin granules. Thus, these nuclear structures may play a role in transport of mRNA to the cytoplasm. / Department of Physiology and Health Science
9

Electron transport and adenosine triphosphatase activities in turnip microsome and soluble fractions

Rungie, John Michael January 1971 (has links)
161 leaves ; ill. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Botany, 1973
10

Electron transport and adenosine triphosphatase activities in turnip microsome and soluble fractions.

Rungie, John Michael. January 1971 (has links) (PDF)
Thesis (Ph.D.) -- University of Adelaide, Dept. of Botany, 1973.

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